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Protein

Alpha-amylase isozyme 3B

Gene

AMY1.6

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Important for breakdown of endosperm starch during germination.

Miscellaneous

Binds starch not only at the active site, but also via accessory binding sites on the protein surface that are important for efficient binding to starch granules and thereby increase enzyme activity.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117Calcium 1By similarity1
Metal bindingi134Calcium 2By similarity1
Metal bindingi137Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi139Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi143Calcium 2By similarity1
Metal bindingi153Calcium 3By similarity1
Metal bindingi164Calcium 1By similarity1
Metal bindingi167Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi168Calcium 3By similarity1
Metal bindingi169Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi172Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi174Calcium 1By similarity1
Metal bindingi174Calcium 3By similarity1
Active sitei205NucleophileBy similarity1
Metal bindingi209Calcium 1; via carbonyl oxygenBy similarity1
Active sitei230Proton donorBy similarity1
Binding sitei232SubstrateBy similarity1
Binding sitei234SubstrateBy similarity1
Binding sitei252SubstrateBy similarity1
Binding sitei293SubstrateBy similarity1
Binding sitei312SubstrateBy similarity1
Sitei313Transition state stabilizerBy similarity1
Binding sitei318SubstrateBy similarity1
Binding sitei397SubstrateBy similarity1
Binding sitei424SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • starch catabolic process Source: Gramene
  • sucrose catabolic process Source: Gramene

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase isozyme 3B (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1.6
Synonyms:AMY3B
Ordered Locus Names:Os09g0457600Curated, LOC_Os09g28420Curated
ORF Names:B1045B05.10, OsJ_29630Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 9

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000000141327 – 438Alpha-amylase isozyme 3BAdd BLAST412

Proteomic databases

PaxDbiP27937
PRIDEiP27937

Expressioni

Tissue specificityi

Germinating seeds.

Developmental stagei

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Gene expression databases

ExpressionAtlasiP27937 differential
GenevisibleiP27937 OS

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi39947.LOC_Os09g28420.1

Structurei

3D structure databases

ProteinModelPortaliP27937
SMRiP27937
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 72Substrate bindingBy similarity3
Regioni77 – 78Substrate bindingBy similarity2
Regioni203 – 208Substrate bindingBy similarity6
Regioni299 – 301Substrate bindingBy similarity3
Regioni402 – 404Substrate bindingBy similarity3
Regioni414 – 420Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
HOGENOMiHOG000239525
InParanoidiP27937
KOiK01176
OMAiNTSPNFA
OrthoDBiEOG093600FS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27937-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKRIASMSS LLLIALLCLS SHLAQAQVLF QGFNWESWKK QGGWYNFLHG
60 70 80 90 100
HVDDIAATGV THVWLPPPSH SVAPQGYMPG RLYDLDASKY GTGAELRSLI
110 120 130 140 150
AAFHSKGIKC VADIVINHRC ADYKDSRGIY CIFEGGTPDS RLDWGPDMIC
160 170 180 190 200
SDDTQYSNGR GHRDTGADFG AAPDIDHLNT RVQTELSDWL NWLKSDVGFD
210 220 230 240 250
GWRLDFAKGY SAAVAKTYVD NTDPSFVVAE IWSNMRYDGN GEPSWNQDGD
260 270 280 290 300
RQELVNWAQA VGGPASAFDF TTKGELQAAV QGELWRMKDG NGKAPGMIGW
310 320 330 340 350
LPEKAVTFID NHDTGSTQNS WPFPSDKVMQ GYAYILTHPG VPCIFYDHVF
360 370 380 390 400
DWNLKQEIST LAAVRSRNEI HPGSKLKILA AEGDVYVAMI DDKVITKIGT
410 420 430
RYDVGNLIPS DFHVVAHGNN YCIWEKSGLR VPAGRHHY
Length:438
Mass (Da):48,592
Last modified:August 1, 1992 - v1
Checksum:iB9DE0DB5ABC63F9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56337 Genomic DNA Translation: CAA39777.1
M24941 Genomic DNA Translation: AAA33897.1
AP005891 Genomic DNA Translation: BAD38368.1
AP008215 Genomic DNA Translation: BAH94599.1
AP014965 Genomic DNA Translation: BAT08442.1
CM000146 Genomic DNA Translation: EAZ44988.1
PIRiS14957
RefSeqiXP_015612113.1, XM_015756627.1

Genome annotation databases

EnsemblPlantsiOs09t0457600-00; Os09t0457600-00; Os09g0457600
GeneIDi9271949
GrameneiOs09t0457600-00; Os09t0457600-00; Os09g0457600
KEGGiosa:9271949

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56337 Genomic DNA Translation: CAA39777.1
M24941 Genomic DNA Translation: AAA33897.1
AP005891 Genomic DNA Translation: BAD38368.1
AP008215 Genomic DNA Translation: BAH94599.1
AP014965 Genomic DNA Translation: BAT08442.1
CM000146 Genomic DNA Translation: EAZ44988.1
PIRiS14957
RefSeqiXP_015612113.1, XM_015756627.1

3D structure databases

ProteinModelPortaliP27937
SMRiP27937
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os09g28420.1

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiP27937
PRIDEiP27937

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOs09t0457600-00; Os09t0457600-00; Os09g0457600
GeneIDi9271949
GrameneiOs09t0457600-00; Os09t0457600-00; Os09g0457600
KEGGiosa:9271949

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
HOGENOMiHOG000239525
InParanoidiP27937
KOiK01176
OMAiNTSPNFA
OrthoDBiEOG093600FS

Gene expression databases

ExpressionAtlasiP27937 differential
GenevisibleiP27937 OS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY3B_ORYSJ
AccessioniPrimary (citable) accession number: P27937
Secondary accession number(s): A0A0N7KQX6, A3BZJ9, Q67U03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 7, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
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Main funding by: National Institutes of Health

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