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Protein

Alpha-amylase isozyme 3E

Gene

AMY1.4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Important for breakdown of endosperm starch during germination.

Miscellaneous

Binds starch not only at the active site, but also via accessory binding sites on the protein surface that are important for efficient binding to starch granules and thereby increase enzyme activity.By similarity

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116Calcium 1By similarity1
Metal bindingi133Calcium 2By similarity1
Metal bindingi136Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi138Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi142Calcium 2By similarity1
Metal bindingi152Calcium 3By similarity1
Metal bindingi163Calcium 1By similarity1
Metal bindingi166Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi168Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi171Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi173Calcium 1By similarity1
Metal bindingi173Calcium 3By similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium 1; via carbonyl oxygenBy similarity1
Active sitei229Proton donorBy similarity1
Binding sitei231SubstrateBy similarity1
Binding sitei233SubstrateBy similarity1
Binding sitei251SubstrateBy similarity1
Binding sitei292SubstrateBy similarity1
Binding sitei311SubstrateBy similarity1
Sitei312Transition state stabilizerBy similarity1
Binding sitei317SubstrateBy similarity1
Binding sitei396SubstrateBy similarity1
Binding sitei423SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • starch catabolic process Source: Gramene
  • sucrose catabolic process Source: Gramene

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase isozyme 3E (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1.4
Synonyms:AMY3E
Ordered Locus Names:Os08g0473600, LOC_Os08g36900
ORF Names:P0013B04.34-1, P0451G12.3-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 8

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000141626 – 437Alpha-amylase isozyme 3EAdd BLAST412

Proteomic databases

PaxDbiP27934
PRIDEiP27934

Expressioni

Tissue specificityi

More abundant in germinating seeds than in young roots, young leaves and callus.

Developmental stagei

Expressed at a high level during germination in the aleurones cells under the control of the plant hormone gibberellic acid and in the developing grains at a low level.

Gene expression databases

ExpressionAtlasiP27934 differential
GenevisibleiP27934 OS

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi39947.LOC_Os08g36900.1

Structurei

3D structure databases

ProteinModelPortaliP27934
SMRiP27934
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 71Substrate bindingBy similarity3
Regioni76 – 77Substrate bindingBy similarity2
Regioni202 – 207Substrate bindingBy similarity6
Regioni298 – 300Substrate bindingBy similarity3
Regioni401 – 403Substrate bindingBy similarity3
Regioni413 – 419Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
HOGENOMiHOG000239525
InParanoidiP27934
KOiK01176
OMAiPSFICGD
OrthoDBiEOG093600FS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P27934-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKHHVTLCC VVFAVLCLAS SLAQAQVLFQ GFNWESWRKQ GGWYNFLHEK
60 70 80 90 100
VEEIASTGAT HVWLPPPSHS VSPQGYMPGR LYDLDASKYG TEAELKSLIE
110 120 130 140 150
AFHDKNVECL ADIVINHRCA DYKDSRGVYC VFEGGTPDGR LDWGPDMICS
160 170 180 190 200
DDTQYSNGRG HRDTGAGFGA APDIDHLNPR VQRELTDWLN WLRTDLGFDG
210 220 230 240 250
WRLDFAKGYS APLARIYVDN TNPTFVVGEI WSSLIYNGDG KPSTNQDADR
260 270 280 290 300
QELVNWVEGV GKPATAFDFT TKGILQAAVQ GELWRLHDGN GKAPGLMGWM
310 320 330 340 350
PDQAVTFVDN HDTGSTQSLW PFPSDKVMQG YAYILTHPGI PCIFYDHVFD
360 370 380 390 400
WNLQHEIATL AEIRSRNGIH AESTLDILKA EGDIYVAMID GKVITKLGPR
410 420 430
YDAGGIIPSD FHVVAHGNDY CVWEKEGLRV PAGRKHY
Length:437
Mass (Da):48,708
Last modified:August 1, 1992 - v1
Checksum:iC0E8276CCEA16602
GO
Isoform 2 (identifier: P27934-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-233: TFVVGEIWSS → ASWGGCPIRP
     234-437: Missing.

Note: No experimental confirmation available.
Show »
Length:233
Mass (Da):26,038
Checksum:i4EFAC00EB1B5078A
GO

Sequence cautioni

The sequence BAD73794 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD73796 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016022224 – 233TFVVGEIWSS → ASWGGCPIRP in isoform 2. 1 Publication10
Alternative sequenceiVSP_016023234 – 437Missing in isoform 2. 1 PublicationAdd BLAST204

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59352 Genomic DNA Translation: AAA33896.1
AP004399 Genomic DNA Translation: BAD09334.1
AP004399 Genomic DNA Translation: BAD73794.1 Sequence problems.
AP004457 Genomic DNA Translation: BAD09374.1
AP004457 Genomic DNA Translation: BAD73796.1 Sequence problems.
AP014964 Genomic DNA Translation: BAT05857.1
AP014964 Genomic DNA Translation: BAT05858.1
AK064071 mRNA No translation available.
AK103413 mRNA No translation available.
PIRiJT0946
RefSeqiXP_015650698.1, XM_015795212.1 [P27934-1]
UniGeneiOs.12593

Genome annotation databases

EnsemblPlantsiOs08t0473600-01; Os08t0473600-01; Os08g0473600 [P27934-1]
Os08t0473600-02; Os08t0473600-02; Os08g0473600 [P27934-2]
GeneIDi4345812
GrameneiOs08t0473600-01; Os08t0473600-01; Os08g0473600 [P27934-1]
Os08t0473600-02; Os08t0473600-02; Os08g0473600 [P27934-2]
KEGGiosa:4345812

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59352 Genomic DNA Translation: AAA33896.1
AP004399 Genomic DNA Translation: BAD09334.1
AP004399 Genomic DNA Translation: BAD73794.1 Sequence problems.
AP004457 Genomic DNA Translation: BAD09374.1
AP004457 Genomic DNA Translation: BAD73796.1 Sequence problems.
AP014964 Genomic DNA Translation: BAT05857.1
AP014964 Genomic DNA Translation: BAT05858.1
AK064071 mRNA No translation available.
AK103413 mRNA No translation available.
PIRiJT0946
RefSeqiXP_015650698.1, XM_015795212.1 [P27934-1]
UniGeneiOs.12593

3D structure databases

ProteinModelPortaliP27934
SMRiP27934
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g36900.1

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiP27934
PRIDEiP27934

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOs08t0473600-01; Os08t0473600-01; Os08g0473600 [P27934-1]
Os08t0473600-02; Os08t0473600-02; Os08g0473600 [P27934-2]
GeneIDi4345812
GrameneiOs08t0473600-01; Os08t0473600-01; Os08g0473600 [P27934-1]
Os08t0473600-02; Os08t0473600-02; Os08g0473600 [P27934-2]
KEGGiosa:4345812

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA
HOGENOMiHOG000239525
InParanoidiP27934
KOiK01176
OMAiPSFICGD
OrthoDBiEOG093600FS

Gene expression databases

ExpressionAtlasiP27934 differential
GenevisibleiP27934 OS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY3E_ORYSJ
AccessioniPrimary (citable) accession number: P27934
Secondary accession number(s): Q5QLB2, Q6ZDD6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 7, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
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