UniProtKB - P27896 (NARQ_ECOLI)
Protein
Nitrate/nitrite sensor protein NarQ
Gene
narQ
Organism
Escherichia coli (strain K12)
Status
Functioni
Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the NarL/NarP proteins. NarQ probably activates NarL and NarP by phosphorylation. NarQ probably negatively regulates the NarL protein by dephosphorylation.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphorelay sensor kinase activity Source: EcoCyc
- protein dimerization activity Source: InterPro
GO - Biological processi
- cellular response to nitrate Source: EcoCyc
- cellular response to nitrite Source: EcoCyc
- nitrate assimilation Source: UniProtKB-KW
- signal transduction by protein phosphorylation Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Nitrate assimilation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:NARQ-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:narQ Ordered Locus Names:b2469, JW2453 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 13 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 14 – 34 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 35 – 146 | PeriplasmicSequence analysisAdd BLAST | 112 | |
Transmembranei | 147 – 167 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 168 – 566 | CytoplasmicSequence analysisAdd BLAST | 399 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074810 | 1 – 566 | Nitrate/nitrite sensor protein NarQAdd BLAST | 566 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 370 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P27896 |
PRIDEi | P27896 |
Interactioni
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4260928, 11 interactors 851287, 2 interactors |
DIPi | DIP-10318N |
IntActi | P27896, 12 interactors |
STRINGi | 511145.b2469 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27896 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 174 – 227 | HAMPPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 364 – 559 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 196 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3850, Bacteria |
HOGENOMi | CLU_000445_20_10_6 |
InParanoidi | P27896 |
PhylomeDBi | P27896 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit |
Gene3Di | 1.20.120.960, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR029095, NarX-like_N IPR042295, NarX-like_N_sf IPR016380, Sig_transdc_His_kin_NarX/NarQ IPR011712, Sig_transdc_His_kin_sub3_dim/P |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF07730, HisKA_3, 1 hit PF13675, PilJ, 1 hit |
PIRSFi | PIRSF003167, STHK_NarX/NarQ, 1 hit |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P27896-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVKRPVSAS LARAFFYIVL LSILSTGIAL LTLASSLRDA EAINIAGSLR
60 70 80 90 100
MQSYRLGYDL QSGSPQLNAH RQLFQQALHS PVLTNLNVWY VPEAVKTRYA
110 120 130 140 150
HLNANWLEMN NRLSKGDLPW YQANINNYVN QIDLFVLALQ HYAERKMLLV
160 170 180 190 200
VAISLAGGIG IFTLVFFTLR RIRHQVVAPL NQLVTASQRI EHGQFDSPPL
210 220 230 240 250
DTNLPNELGL LAKTFNQMSS ELHKLYRSLE ASVEEKTRDL HEAKRRLEVL
260 270 280 290 300
YQCSQALNTS QIDVHCFRHI LQIVRDNEAA EYLELNVGEN WRISEGQPNP
310 320 330 340 350
ELPMQILPVT MQETVYGELH WQNSHVSSSE PLLNSVSSML GRGLYFNQAQ
360 370 380 390 400
KHFQQLLLME ERATIARELH DSLAQVLSYL RIQLTLLKRS IPEDNATAQS
410 420 430 440 450
IMADFSQALN DAYRQLRELL TTFRLTLQQA DLPSALREML DTLQNQTSAK
460 470 480 490 500
LTLDCRLPTL ALDAQMQVHL LQIIREAVLN AMKHANASEI AVSCVTAPDG
510 520 530 540 550
NHTVYIRDNG IGIGEPKEPE GHYGLNIMRE RAERLGGTLT FSQPSGGGTL
560
VSISFRSAEG EESQLM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94724 Genomic DNA Translation: AAA24202.1 X65714 Genomic DNA Translation: CAA46630.1 U00096 Genomic DNA Translation: AAC75522.1 AP009048 Genomic DNA Translation: BAA16343.1 U12598 Genomic DNA Translation: AAA20583.1 |
PIRi | D65022 |
RefSeqi | NP_416964.1, NC_000913.3 WP_001300881.1, NZ_CP014272.1 |
Genome annotation databases
EnsemblBacteriai | AAC75522; AAC75522; b2469 BAA16343; BAA16343; BAA16343 |
GeneIDi | 57729465 946948 |
KEGGi | ecj:JW2453 eco:b2469 |
PATRICi | fig|511145.12.peg.2563 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94724 Genomic DNA Translation: AAA24202.1 X65714 Genomic DNA Translation: CAA46630.1 U00096 Genomic DNA Translation: AAC75522.1 AP009048 Genomic DNA Translation: BAA16343.1 U12598 Genomic DNA Translation: AAA20583.1 |
PIRi | D65022 |
RefSeqi | NP_416964.1, NC_000913.3 WP_001300881.1, NZ_CP014272.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IJI | X-ray | 1.94 | A | 1-230 | [»] | |
5JEF | X-ray | 2.42 | A/B | 1-230 | [»] | |
5JEQ | X-ray | 1.90 | A | 1-230 | [»] | |
5JGP | X-ray | 2.70 | A | 1-230 | [»] | |
6XYN | X-ray | 2.30 | A | 1-230 | [»] | |
6YUE | X-ray | 2.40 | A | 1-230 | [»] | |
SMRi | P27896 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260928, 11 interactors 851287, 2 interactors |
DIPi | DIP-10318N |
IntActi | P27896, 12 interactors |
STRINGi | 511145.b2469 |
Proteomic databases
PaxDbi | P27896 |
PRIDEi | P27896 |
Genome annotation databases
EnsemblBacteriai | AAC75522; AAC75522; b2469 BAA16343; BAA16343; BAA16343 |
GeneIDi | 57729465 946948 |
KEGGi | ecj:JW2453 eco:b2469 |
PATRICi | fig|511145.12.peg.2563 |
Organism-specific databases
EchoBASEi | EB1429 |
Phylogenomic databases
eggNOGi | COG3850, Bacteria |
HOGENOMi | CLU_000445_20_10_6 |
InParanoidi | P27896 |
PhylomeDBi | P27896 |
Enzyme and pathway databases
BioCyci | EcoCyc:NARQ-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
PROi | PR:P27896 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit |
Gene3Di | 1.20.120.960, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR029095, NarX-like_N IPR042295, NarX-like_N_sf IPR016380, Sig_transdc_His_kin_NarX/NarQ IPR011712, Sig_transdc_His_kin_sub3_dim/P |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF07730, HisKA_3, 1 hit PF13675, PilJ, 1 hit |
PIRSFi | PIRSF003167, STHK_NarX/NarQ, 1 hit |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NARQ_ECOLI | |
Accessioni | P27896Primary (citable) accession number: P27896 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references