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Entry version 129 (02 Dec 2020)
Sequence version 2 (30 May 2000)
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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi564ZincUniRule annotation1
Metal bindingi568ZincUniRule annotation1
Metal bindingi676ZincUniRule annotation1
Metal bindingi680ZincUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:R01798
ORF Names:SMc00475
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri266834 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001976 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000751871 – 887Alanine--tRNA ligaseAdd BLAST887

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
266834.SMc00475

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P27866

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0013, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004485_1_1_5

Identification of Orthologs from Complete Genome Data

More...
OMAi
YHHTMFE

Family and domain databases

HAMAP database of protein families

More...
HAMAPi
MF_00036_B, Ala_tRNA_synth_B, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002318, Ala-tRNA-lgiase_IIc
IPR018162, Ala-tRNA-ligase_IIc_anticod-bd
IPR018165, Ala-tRNA-synth_IIc_core
IPR018164, Ala-tRNA-synth_IIc_N
IPR023033, Ala_tRNA_ligase_euk/bac
IPR003156, DHHA1_dom
IPR018163, Thr/Ala-tRNA-synth_IIc_edit
IPR009000, Transl_B-barrel_sf
IPR012947, tRNA_SAD

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02272, DHHA1, 1 hit
PF01411, tRNA-synt_2c, 1 hit
PF07973, tRNA_SAD, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00980, TRNASYNTHALA

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00863, tRNA_SAD, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF101353, SSF101353, 1 hit
SSF50447, SSF50447, 1 hit
SSF55186, SSF55186, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00344, alaS, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P27866-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGVNEIRSM FLDYFRKNGH EIVSSSPLVP RNDPTLMFTN AGMVQFKNVF
60 70 80 90 100
TGLEQRPYST AATAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD
110 120 130 140 150
YFKERAIELA WNLITKEYGL DAKRLLVTVY HTDDEAFGLW KKIAGLSDDR
160 170 180 190 200
IIRIATSDNF WAMGDTGPCG PCSEIFYDHG DHIWGGPPGS AEEDGDRFIE
210 220 230 240 250
IWNLVFMQYE QITKEERVDL PRPSIDTGMG LERVAAVLQG QHDNYDIDLF
260 270 280 290 300
RALIAASEEA TGVKAEGDRR ASHRVIADHL RSSAFLIADG VLPSNEGRGY
310 320 330 340 350
VLRRIMRRAM RHAQLLGARD PLMWKLLPAL VGQMGRAYPE LVRAEALISE
360 370 380 390 400
TLKLEETRFR KTLERGLNLL AEASADLSEG DQFNGETAFK LYDTYGFPLD
410 420 430 440 450
LTQDALRAKG IGVDTDAFTA AMQRQKAEAR ANWAGSGEAA TETIWFELRD
460 470 480 490 500
KHGATDFLGY DTESAEGVIL AIVKDGAVVE SAAKGENVQL VLNQTPFYGE
510 520 530 540 550
SGGQVGDTGV ITTETGKLTV TDTQKRGEGL FVHYCTVEEG SVKTGEAAAL
560 570 580 590 600
TVDHARRARL RANHSATHLL HEALREVLGT HVAQKGSLVA PERLRFDVSH
610 620 630 640 650
PKPMTAEELK IVEEMANEII VQNTPVVTRL MSVDDAIAEG AMALFGEKYG
660 670 680 690 700
DEVRVVSMGQ GLRGSKAGKP YSVELCGGTH VSATGDIGLV RVVSESAVGA
710 720 730 740 750
GVRRVEALTG EAARAYLGEQ DERVKTLAAA LKVQPADVLG RVEALLDERR
760 770 780 790 800
KLERELTEAK KKLALAGDGQ NGSGDAARDI GGVRFLGRVV SGVEPKDLKS
810 820 830 840 850
LADDGKKSLG SGVVAFVGVS GDGKASAVVA VTDDLTSKVS AVDLVRVASA
860 870 880
ALGGKGGGGR PDMAQAGGPD GGRAAEAIEA VAGALAG
Length:887
Mass (Da):95,434
Last modified:May 30, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i439810AAFF101A28
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti135 – 136EA → DT in CAA42581 (PubMed:1896024).Curated2
Sequence conflicti150R → H in CAA42581 (PubMed:1896024).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL591688 Genomic DNA Translation: CAC46377.1
X59957 Genomic DNA Translation: CAA42581.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S16899

NCBI Reference Sequences

More...
RefSeqi
NP_385904.1, NC_003047.1
WP_010969477.1, NC_003047.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAC46377; CAC46377; SMc00475

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sme:SMc00475

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|266834.11.peg.3238

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591688 Genomic DNA Translation: CAC46377.1
X59957 Genomic DNA Translation: CAA42581.1
PIRiS16899
RefSeqiNP_385904.1, NC_003047.1
WP_010969477.1, NC_003047.1

3D structure databases

SMRiP27866
ModBaseiSearch...

Protein-protein interaction databases

STRINGi266834.SMc00475

Genome annotation databases

EnsemblBacteriaiCAC46377; CAC46377; SMc00475
KEGGisme:SMc00475
PATRICifig|266834.11.peg.3238

Phylogenomic databases

eggNOGiCOG0013, Bacteria
HOGENOMiCLU_004485_1_1_5
OMAiYHHTMFE

Family and domain databases

HAMAPiMF_00036_B, Ala_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR002318, Ala-tRNA-lgiase_IIc
IPR018162, Ala-tRNA-ligase_IIc_anticod-bd
IPR018165, Ala-tRNA-synth_IIc_core
IPR018164, Ala-tRNA-synth_IIc_N
IPR023033, Ala_tRNA_ligase_euk/bac
IPR003156, DHHA1_dom
IPR018163, Thr/Ala-tRNA-synth_IIc_edit
IPR009000, Transl_B-barrel_sf
IPR012947, tRNA_SAD
PfamiView protein in Pfam
PF02272, DHHA1, 1 hit
PF01411, tRNA-synt_2c, 1 hit
PF07973, tRNA_SAD, 1 hit
PRINTSiPR00980, TRNASYNTHALA
SMARTiView protein in SMART
SM00863, tRNA_SAD, 1 hit
SUPFAMiSSF101353, SSF101353, 1 hit
SSF50447, SSF50447, 1 hit
SSF55186, SSF55186, 1 hit
TIGRFAMsiTIGR00344, alaS, 1 hit
PROSITEiView protein in PROSITE
PS50860, AA_TRNA_LIGASE_II_ALA, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYA_RHIME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P27866
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 30, 2000
Last modified: December 2, 2020
This is version 129 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
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