UniProtKB - P27829 (WECC_ECOLI)
Protein
UDP-N-acetyl-D-mannosamine dehydrogenase
Gene
wecC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the four-electron oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc), reducing NAD+ and releasing UDP-N-acetylmannosaminuronic acid (UDP-ManNAcA).UniRule annotation1 Publication
Catalytic activityi
- H2O + 2 NAD+ + UDP-N-acetyl-α-D-mannosamine = 3 H+ + 2 NADH + UDP-N-acetyl-α-D-mannosaminouronateUniRule annotation1 PublicationEC:1.1.1.336UniRule annotation1 Publication
Activity regulationi
Activated by N-acetylglucosamine-1-P or K+ at low UDP-ManNAc concentrations.1 Publication
Kineticsi
- KM=0.22 mM for UDP-ManNAc (in the presence of 1 mM N-acetylglucosamine-1-P)1 Publication
- KM=0.11 mM for UDP-ManNAc (in the presence of 150 mM KCl)1 Publication
- KM=0.38 mM for UDP-ManNAc (in the absence of activators)1 Publication
- KM=0.21 mM for NAD+1 Publication
pH dependencei
Optimum pH is 10.0.1 Publication
: enterobacterial common antigen biosynthesis Pathwayi
This protein is involved in the pathway enterobacterial common antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway enterobacterial common antigen biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 212 | Proton donor/acceptorUniRule annotationBy similarity | 1 | |
Binding sitei | 212 | SubstrateUniRule annotationBy similarity | 1 | |
Binding sitei | 263 | Substrate; via amide nitrogenUniRule annotationBy similarity | 1 | |
Active sitei | 266 | NucleophileUniRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 15 | NADUniRule annotationBy similarity | 6 |
GO - Molecular functioni
- NAD binding Source: InterPro
- oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: InterPro
- UDP-N-acetyl-D-mannosamine dehydrogenase activity Source: EcoCyc
GO - Biological processi
- enterobacterial common antigen biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:UDPMANNACADEHYDROG-MONOMER MetaCyc:UDPMANNACADEHYDROG-MONOMER |
SABIO-RKi | P27829 |
UniPathwayi | UPA00566 |
Names & Taxonomyi
Protein namesi | Recommended name: UDP-N-acetyl-D-mannosamine dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.336UniRule annotation1 Publication)Alternative name(s): UDP-ManNAc 6-dehydrogenaseUniRule annotationCurated |
Gene namesi | Name:wecCUniRule annotation Synonyms:rffD1 Publication Ordered Locus Names:b3787, JW5599 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutants do not synthesize Und-PP-GlcNAc-ManNAcA (lipid II) and enterobacterial common antigen (ECA).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074077 | 1 – 420 | UDP-N-acetyl-D-mannosamine dehydrogenaseAdd BLAST | 420 |
Proteomic databases
jPOSTi | P27829 |
PaxDbi | P27829 |
PRIDEi | P27829 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262604, 229 interactors |
STRINGi | 511145.b3787 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 160 – 161 | Substrate bindingUniRule annotationBy similarity | 2 | |
Regioni | 216 – 219 | Substrate bindingUniRule annotationBy similarity | 4 | |
Regioni | 250 – 252 | Substrate binding; shared with dimeric partnerUniRule annotationBy similarity | 3 | |
Regioni | 330 – 331 | Substrate bindingUniRule annotationBy similarity | 2 |
Sequence similaritiesi
Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. WecC subfamily.UniRule annotationCurated
Phylogenomic databases
eggNOGi | COG0677, Bacteria |
HOGENOMi | CLU_023810_3_2_6 |
InParanoidi | P27829 |
PhylomeDBi | P27829 |
Family and domain databases
HAMAPi | MF_02029, WecC_RffD, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR017476, UDP-Glc/GDP-Man IPR014027, UDP-Glc/GDP-Man_DH_C IPR036220, UDP-Glc/GDP-Man_DH_C_sf IPR014026, UDP-Glc/GDP-Man_DH_dimer IPR001732, UDP-Glc/GDP-Man_DH_N IPR028359, UDP_ManNAc/GlcNAc_DH IPR032891, WecC |
Pfami | View protein in Pfam PF00984, UDPG_MGDP_dh, 1 hit PF03720, UDPG_MGDP_dh_C, 1 hit PF03721, UDPG_MGDP_dh_N, 1 hit |
PIRSFi | PIRSF500136, UDP_ManNAc_DH, 1 hit PIRSF000124, UDPglc_GDPman_dh, 1 hit |
SMARTi | View protein in SMART SM00984, UDPG_MGDP_dh_C, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit SSF52413, SSF52413, 1 hit |
TIGRFAMsi | TIGR03026, NDP-sugDHase, 1 hit |
i Sequence
Sequence statusi: Complete.
P27829-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSFATISVIG LGYIGLPTAA AFASRQKQVI GVDINQHAVD TINRGEIHIV
60 70 80 90 100
EPDLASVVKT AVEGGFLRAS TTPVEADAWL IAVPTPFKGD HEPDMTYVES
110 120 130 140 150
AARSIAPVLK KGALVILEST SPVGSTEKMA EWLAEMRPDL TFPQQVGEQA
160 170 180 190 200
DVNIAYCPER VLPGQVMVEL IKNDRVIGGM TPVCSARASE LYKIFLEGEC
210 220 230 240 250
VVTNSRTAEM CKLTENSFRD VNIAFANELS LICADQGINV WELIRLANRH
260 270 280 290 300
PRVNILQPGP GVGGHCIAVD PWFIVAQNPQ QARLIRTARE VNDHKPFWVI
310 320 330 340 350
DQVKAAVADC LAATDKRASE LKIACFGLAF KPNIDDLRES PAMEIAELIA
360 370 380 390 400
QWHSGETLVV EPNIHQLPKK LTGLCTLAQL DEALATADVL VMLVDHSQFK
410 420
VINGDNVHQQ YVVDAKGVWR
Sequence cautioni
The sequence AAA67587 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 78 | A → R in AAA67587 (PubMed:1379743).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L18799 Unassigned DNA No translation available. M87049 Genomic DNA Translation: AAA67587.1 Frameshift. U00096 Genomic DNA Translation: AAT48212.1 AP009048 Genomic DNA Translation: BAE77511.1 |
PIRi | F65182 |
RefSeqi | WP_000006621.1, NZ_STEB01000021.1 YP_026254.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48212; AAT48212; b3787 BAE77511; BAE77511; BAE77511 |
GeneIDi | 58459603 948977 |
KEGGi | ecj:JW5599 eco:b3787 |
PATRICi | fig|1411691.4.peg.2919 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L18799 Unassigned DNA No translation available. M87049 Genomic DNA Translation: AAA67587.1 Frameshift. U00096 Genomic DNA Translation: AAT48212.1 AP009048 Genomic DNA Translation: BAE77511.1 |
PIRi | F65182 |
RefSeqi | WP_000006621.1, NZ_STEB01000021.1 YP_026254.1, NC_000913.3 |
3D structure databases
SMRi | P27829 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262604, 229 interactors |
STRINGi | 511145.b3787 |
Proteomic databases
jPOSTi | P27829 |
PaxDbi | P27829 |
PRIDEi | P27829 |
Genome annotation databases
EnsemblBacteriai | AAT48212; AAT48212; b3787 BAE77511; BAE77511; BAE77511 |
GeneIDi | 58459603 948977 |
KEGGi | ecj:JW5599 eco:b3787 |
PATRICi | fig|1411691.4.peg.2919 |
Organism-specific databases
EchoBASEi | EB1421 |
Phylogenomic databases
eggNOGi | COG0677, Bacteria |
HOGENOMi | CLU_023810_3_2_6 |
InParanoidi | P27829 |
PhylomeDBi | P27829 |
Enzyme and pathway databases
UniPathwayi | UPA00566 |
BioCyci | EcoCyc:UDPMANNACADEHYDROG-MONOMER MetaCyc:UDPMANNACADEHYDROG-MONOMER |
SABIO-RKi | P27829 |
Miscellaneous databases
PROi | PR:P27829 |
Family and domain databases
HAMAPi | MF_02029, WecC_RffD, 1 hit |
InterProi | View protein in InterPro IPR008927, 6-PGluconate_DH-like_C_sf IPR036291, NAD(P)-bd_dom_sf IPR017476, UDP-Glc/GDP-Man IPR014027, UDP-Glc/GDP-Man_DH_C IPR036220, UDP-Glc/GDP-Man_DH_C_sf IPR014026, UDP-Glc/GDP-Man_DH_dimer IPR001732, UDP-Glc/GDP-Man_DH_N IPR028359, UDP_ManNAc/GlcNAc_DH IPR032891, WecC |
Pfami | View protein in Pfam PF00984, UDPG_MGDP_dh, 1 hit PF03720, UDPG_MGDP_dh_C, 1 hit PF03721, UDPG_MGDP_dh_N, 1 hit |
PIRSFi | PIRSF500136, UDP_ManNAc_DH, 1 hit PIRSF000124, UDPglc_GDPman_dh, 1 hit |
SMARTi | View protein in SMART SM00984, UDPG_MGDP_dh_C, 1 hit |
SUPFAMi | SSF48179, SSF48179, 1 hit SSF51735, SSF51735, 1 hit SSF52413, SSF52413, 1 hit |
TIGRFAMsi | TIGR03026, NDP-sugDHase, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WECC_ECOLI | |
Accessioni | P27829Primary (citable) accession number: P27829 Secondary accession number(s): Q2M895 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 167 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families