UniProtKB - P27825 (CALX_YEAST)
Protein
Calnexin homolog
Gene
CNE1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.1 Publication
Miscellaneous
In contrast with other calnexin, yeast CNE1 does not seem to bind calcium.
Present with 4760 molecules/cell in log phase SD medium.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 131 | CarbohydrateBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- carbohydrate binding Source: UniProtKB-KW
- unfolded protein binding Source: SGD
GO - Biological processi
- endoplasmic reticulum unfolded protein response Source: GO_Central
- protein folding Source: SGD
- ubiquitin-dependent ERAD pathway Source: SGD
Keywordsi
Molecular function | Chaperone |
Ligand | Lectin |
Enzyme and pathway databases
Reactomei | R-SCE-901042, Calnexin/calreticulin cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Calnexin homolog |
Gene namesi | Name:CNE1 Ordered Locus Names:YAL058W ORF Names:FUN48 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000054, CNE1 |
VEuPathDBi | FungiDB:YAL058W |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
- endoplasmic reticulum membrane Source: GO_Central
- integral component of endoplasmic reticulum membrane Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 481 | LumenalSequence analysisAdd BLAST | 462 | |
Transmembranei | 482 – 502 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000004208 | 20 – 502 | Calnexin homologAdd BLAST | 483 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 25 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 104 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 125 ↔ 161 | By similarity | ||
Glycosylationi | 296 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 332 ↔ 338 | By similarity | ||
Glycosylationi | 416 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 425 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P27825 |
PaxDbi | P27825 |
PRIDEi | P27825 |
TopDownProteomicsi | P27825 |
Interactioni
Subunit structurei
Interacts with MPD1.
1 PublicationGO - Molecular functioni
- unfolded protein binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 31772, 201 interactors |
DIPi | DIP-4121N |
STRINGi | 4932.YAL058W |
Miscellaneous databases
RNActi | P27825, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 250 – 261 | 1-1Add BLAST | 12 | |
Repeati | 267 – 278 | 1-2Add BLAST | 12 | |
Repeati | 286 – 297 | 1-3Add BLAST | 12 | |
Repeati | 305 – 316 | 1-4Add BLAST | 12 | |
Repeati | 320 – 330 | 2-1Add BLAST | 11 | |
Repeati | 339 – 349 | 2-2Add BLAST | 11 | |
Repeati | 353 – 363 | 2-3Add BLAST | 11 | |
Repeati | 367 – 377 | 2-4Add BLAST | 11 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 248 – 381 | P domain (Extended arm)By similarityAdd BLAST | 134 | |
Regioni | 250 – 316 | 4 X approximate repeatsAdd BLAST | 67 | |
Regioni | 320 – 377 | 4 X approximate repeatsAdd BLAST | 58 |
Sequence similaritiesi
Belongs to the calreticulin family.Curated
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0675, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_1_2_1 |
InParanoidi | P27825 |
OMAi | SFWEHFQ |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P27825-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFSAYLWWL FLNLALVKGT SLLSNVTLAE DSFWEHFQAY TNTKHLNQEW
60 70 80 90 100
ITSEAVNNEG SKIYGAQWRL SQGRLQGSAW DKGIAVRTGN AAAMIGHLLE
110 120 130 140 150
TPINVSETDT LVVQYEIKLD NSLTCGGAFI KLMSGFMNVE ALKHYAPDTE
160 170 180 190 200
GVELVFGPDY CAPEINGVQF AINKVDKITH ESKLRYLQEM PLSKLTDTSQ
210 220 230 240 250
SHLYTLIIDE SAQSFQILID GKTVMVREHI EDKKKVNFEP PITPPLMIPD
260 270 280 290 300
VSVAKPHDWD DRIRIPDPEA VKLSDRDERD PLMIPHPDGT EPPEWNSSIP
310 320 330 340 350
EYILDPNAQK PSWWKELEHG EWIPPMIKNP LCTAERGCGQ QIPGLINNAK
360 370 380 390 400
YKGPGELNEI INPNYMGEWH PPEIENPLYY EEQHPLRIEN VISGVILEFW
410 420 430 440 450
SGSPNMLISN IYVGKNVTEA QIIGNKTWLM RDRAFRGSDG PTERKFMNSR
460 470 480 490 500
LGNLQTTFHN ERESPNPFDR IIDRILEQPL KFVLTAAVVL LTTSVLCCVV
FT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66470 Genomic DNA Translation: CAA47100.1 L11012 Genomic DNA Translation: AAA65967.1 U12980 Genomic DNA Translation: AAC04976.1 AY692920 Genomic DNA Translation: AAT92939.1 BK006935 Genomic DNA Translation: DAA06931.1 |
PIRi | S29347 |
RefSeqi | NP_009343.1, NM_001178200.1 |
Genome annotation databases
EnsemblFungii | YAL058W_mRNA; YAL058W; YAL058W |
GeneIDi | 851241 |
KEGGi | sce:YAL058W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66470 Genomic DNA Translation: CAA47100.1 L11012 Genomic DNA Translation: AAA65967.1 U12980 Genomic DNA Translation: AAC04976.1 AY692920 Genomic DNA Translation: AAT92939.1 BK006935 Genomic DNA Translation: DAA06931.1 |
PIRi | S29347 |
RefSeqi | NP_009343.1, NM_001178200.1 |
3D structure databases
SMRi | P27825 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 31772, 201 interactors |
DIPi | DIP-4121N |
STRINGi | 4932.YAL058W |
Proteomic databases
MaxQBi | P27825 |
PaxDbi | P27825 |
PRIDEi | P27825 |
TopDownProteomicsi | P27825 |
Genome annotation databases
EnsemblFungii | YAL058W_mRNA; YAL058W; YAL058W |
GeneIDi | 851241 |
KEGGi | sce:YAL058W |
Organism-specific databases
SGDi | S000000054, CNE1 |
VEuPathDBi | FungiDB:YAL058W |
Phylogenomic databases
eggNOGi | KOG0675, Eukaryota |
GeneTreei | ENSGT00950000182915 |
HOGENOMi | CLU_018224_1_2_1 |
InParanoidi | P27825 |
OMAi | SFWEHFQ |
Enzyme and pathway databases
Reactomei | R-SCE-901042, Calnexin/calreticulin cycle |
Miscellaneous databases
PROi | PR:P27825 |
RNActi | P27825, protein |
Family and domain databases
Gene3Di | 2.10.250.10, 1 hit |
InterProi | View protein in InterPro IPR001580, Calret/calnex IPR018124, Calret/calnex_CS IPR009033, Calreticulin/calnexin_P_dom_sf IPR013320, ConA-like_dom_sf |
PANTHERi | PTHR11073, PTHR11073, 1 hit |
Pfami | View protein in Pfam PF00262, Calreticulin, 1 hit |
PRINTSi | PR00626, CALRETICULIN |
SUPFAMi | SSF49899, SSF49899, 1 hit SSF63887, SSF63887, 1 hit |
PROSITEi | View protein in PROSITE PS00803, CALRETICULIN_1, 1 hit PS00804, CALRETICULIN_2, 1 hit PS00805, CALRETICULIN_REPEAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CALX_YEAST | |
Accessioni | P27825Primary (citable) accession number: P27825 Secondary accession number(s): D6VPG1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | February 10, 2021 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome I
Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names - SIMILARITY comments
Index of protein domains and families