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Protein

Pepsin F

Gene
N/A
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Catalytic activityi

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation1
Active sitei275PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease
Biological processDigestion

Protein family/group databases

MEROPSiA01.051

Names & Taxonomyi

Protein namesi
Recommended name:
Pepsin F (EC:3.4.23.1)
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
PropeptideiPRO_000002603616 – 58Activation peptideAdd BLAST43
ChainiPRO_000002603759 – 388Pepsin FAdd BLAST330

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi266 ↔ 270By similarity
Disulfide bondi309 ↔ 343By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiP27823

Expressioni

Developmental stagei

Early postnatal.

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006601

Structurei

3D structure databases

ProteinModelPortaliP27823
SMRiP27823
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 385Peptidase A1PROSITE-ProRule annotationAdd BLAST312

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
HOGENOMiHOG000197681
HOVERGENiHBG000482
KOiK06002

Family and domain databases

Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27823-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKWLGLLGLV ALSECLVTIP LMKVKSMREN LRENDILLDY LEKHPYRPTY
60 70 80 90 100
KLLSGQQDPD VSFEPLRNYL DLAYIGIISI GTPPQEFKVV LDTGSADLWV
110 120 130 140 150
PSIYCSSPAC GKHNTFNPLL SSTFLVSGRP INIVYGSGRM SGFLAYDTVQ
160 170 180 190 200
IAGLVDVAQA FGLSLQEPGK FMEYAVFDGI LGLSYPSLSF EGITPVFDNL
210 220 230 240 250
WAQGLISQNL FAFYLSSKEE RGSMLMLGGV DPSYYSGDLH WVPVSRPLYW
260 270 280 290 300
QLAVDRISMN GEAIGCDSGC QGIVDTGTSL LIGPRDPVLN IQKIINAQHS
310 320 330 340 350
HGGEYIIDCD TISTLPDIIF TIDGVDYPVP ASAYIRKSSV HGCYSNFDES
360 370 380
AAHESEPYEV WVLGDVFLRL YFTVFDRANN RIGLAPAV
Length:388
Mass (Da):42,786
Last modified:August 1, 1992 - v1
Checksum:i24792BE393594B3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59238 mRNA Translation: AAA31440.1
PIRiA38302
RefSeqiNP_001076165.1, NM_001082696.1
UniGeneiOcu.1945

Genome annotation databases

GeneIDi100009429
KEGGiocu:100009429

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59238 mRNA Translation: AAA31440.1
PIRiA38302
RefSeqiNP_001076165.1, NM_001082696.1
UniGeneiOcu.1945

3D structure databases

ProteinModelPortaliP27823
SMRiP27823
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006601

Protein family/group databases

MEROPSiA01.051

Proteomic databases

PRIDEiP27823

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009429
KEGGiocu:100009429

Organism-specific databases

CTDi5222

Phylogenomic databases

eggNOGiKOG1339 Eukaryota
ENOG410XNV7 LUCA
HOGENOMiHOG000197681
HOVERGENiHBG000482
KOiK06002

Family and domain databases

Gene3Di2.40.70.10, 2 hits
InterProiView protein in InterPro
IPR001461 Aspartic_peptidase_A1
IPR001969 Aspartic_peptidase_AS
IPR012848 Aspartic_peptidase_N
IPR033121 PEPTIDASE_A1
IPR021109 Peptidase_aspartic_dom_sf
PANTHERiPTHR13683 PTHR13683, 1 hit
PfamiView protein in Pfam
PF07966 A1_Propeptide, 1 hit
PF00026 Asp, 1 hit
PRINTSiPR00792 PEPSIN
SUPFAMiSSF50630 SSF50630, 1 hit
PROSITEiView protein in PROSITE
PS00141 ASP_PROTEASE, 2 hits
PS51767 PEPTIDASE_A1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEPAF_RABIT
AccessioniPrimary (citable) accession number: P27823
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 22, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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