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UniProtKB - P27809 (KRE2_YEAST)
Protein
Glycolipid 2-alpha-mannosyltransferase
Gene
KRE2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Mannosyltransferase that transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage. Required for the attachment of the third mannose residue of O-linked saccharides.
Miscellaneous
Present with 13000 molecules/cell in log phase SD medium.1 Publication
Cofactori
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 329 | NucleophileSequence analysis | 1 |
GO - Molecular functioni
- alpha-1,2-mannosyltransferase activity Source: SGD
GO - Biological processi
- cell wall mannoprotein biosynthetic process Source: SGD
- mannosylation Source: GO_Central
- N-glycan processing Source: SGD
- protein N-linked glycosylation Source: GO_Central
- protein O-linked glycosylation Source: SGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT15, Glycosyltransferase Family 15 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycolipid 2-alpha-mannosyltransferase (EC:2.4.1.-)Alternative name(s): Alpha-1,2-mannosyltransferase |
Gene namesi | Name:KRE2 Synonyms:MNT1 Ordered Locus Names:YDR483W ORF Names:D8035.26 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002891, KRE2 |
VEuPathDBi | FungiDB:YDR483W |
Subcellular locationi
Golgi apparatus
Golgi apparatus
- Golgi apparatus Source: SGD
- Golgi medial cisterna Source: CACAO
- Golgi membrane Source: UniProtKB-SubCell
Vacuole
- fungal-type vacuole membrane Source: SGD
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 30 | Helical; Signal-anchor for type II membrane proteinAdd BLAST | 19 | |
Topological domaini | 31 – 442 | LumenalSequence analysisAdd BLAST | 412 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208241 | 1 – 442 | Glycolipid 2-alpha-mannosyltransferaseAdd BLAST | 442 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 197 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | P27809 |
PaxDbi | P27809 |
PRIDEi | P27809 |
PTM databases
iPTMneti | P27809 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32535, 57 interactors |
DIPi | DIP-5373N |
IntActi | P27809, 11 interactors |
STRINGi | 4932.YDR483W |
Miscellaneous databases
RNActi | P27809, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27809 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27809 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 31 – 118 | Stem regionAdd BLAST | 88 | |
Regioni | 68 – 95 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 119 – 442 | CatalyticAdd BLAST | 324 |
Sequence similaritiesi
Belongs to the glycosyltransferase 15 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4472, Eukaryota |
GeneTreei | ENSGT00940000176287 |
HOGENOMi | CLU_024327_1_0_1 |
InParanoidi | P27809 |
OMAi | DPFQYMQ |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002685, Glyco_trans_15 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR31121, PTHR31121, 1 hit |
Pfami | View protein in Pfam PF01793, Glyco_transf_15, 1 hit |
PIRSFi | PIRSF018153, Glyco_trans_15, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
P27809-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALFLSKRLL RFTVIAGAVI VLLLTLNSNS RTQQYIPSSI SAAFDFTSGS
60 70 80 90 100
ISPEQQVISE ENDAKKLEQS ALNSEASEDS EAMDEESKAL KAAAEKADAP
110 120 130 140 150
IDTKTTMDYI TPSFANKAGK PKACYVTLVR NKELKGLLSS IKYVENKINK
160 170 180 190 200
KFPYPWVFLN DEPFTEEFKE AVTKAVSSEV KFGILPKEHW SYPEWINQTK
210 220 230 240 250
AAEIRADAAT KYIYGGSESY RHMCRYQSGF FWRHELLEEY DWYWRVEPDI
260 270 280 290 300
KLYCDINYDV FKWMQENEKV YGFTVSIHEY EVTIPTLWQT SMDFIKKNPE
310 320 330 340 350
YLDENNLMSF LSNDNGKTYN LCHFWSNFEI ANLNLWRSPA YREYFDTLDH
360 370 380 390 400
QGGFFYERWG DAPVHSIAAA LFLPKDKIHY FSDIGYHHPP YDNCPLDKEV
410 420 430 440
YNSNNCECDQ GNDFTFQGYS CGKEYYDAQG LVKPKNWKKF RE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81110 Genomic DNA Translation: AAA34784.1 X62647 Genomic DNA Translation: CAA44516.1 U33050 Genomic DNA Translation: AAB64906.1 Z29988 Genomic DNA Translation: CAA82879.1 BK006938 Genomic DNA Translation: DAA12316.1 |
PIRi | S32592 |
RefSeqi | NP_010771.1, NM_001180791.1 |
Genome annotation databases
EnsemblFungii | YDR483W_mRNA; YDR483W; YDR483W |
GeneIDi | 852094 |
KEGGi | sce:YDR483W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81110 Genomic DNA Translation: AAA34784.1 X62647 Genomic DNA Translation: CAA44516.1 U33050 Genomic DNA Translation: AAB64906.1 Z29988 Genomic DNA Translation: CAA82879.1 BK006938 Genomic DNA Translation: DAA12316.1 |
PIRi | S32592 |
RefSeqi | NP_010771.1, NM_001180791.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1S4N | X-ray | 2.01 | A/B | 97-442 | [»] | |
1S4O | X-ray | 2.01 | A/B | 97-442 | [»] | |
1S4P | X-ray | 2.01 | A/B | 97-442 | [»] | |
SMRi | P27809 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32535, 57 interactors |
DIPi | DIP-5373N |
IntActi | P27809, 11 interactors |
STRINGi | 4932.YDR483W |
Protein family/group databases
CAZyi | GT15, Glycosyltransferase Family 15 |
PTM databases
iPTMneti | P27809 |
Proteomic databases
MaxQBi | P27809 |
PaxDbi | P27809 |
PRIDEi | P27809 |
Genome annotation databases
EnsemblFungii | YDR483W_mRNA; YDR483W; YDR483W |
GeneIDi | 852094 |
KEGGi | sce:YDR483W |
Organism-specific databases
SGDi | S000002891, KRE2 |
VEuPathDBi | FungiDB:YDR483W |
Phylogenomic databases
eggNOGi | KOG4472, Eukaryota |
GeneTreei | ENSGT00940000176287 |
HOGENOMi | CLU_024327_1_0_1 |
InParanoidi | P27809 |
OMAi | DPFQYMQ |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
Miscellaneous databases
EvolutionaryTracei | P27809 |
PROi | PR:P27809 |
RNActi | P27809, protein |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR002685, Glyco_trans_15 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR31121, PTHR31121, 1 hit |
Pfami | View protein in Pfam PF01793, Glyco_transf_15, 1 hit |
PIRSFi | PIRSF018153, Glyco_trans_15, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KRE2_YEAST | |
Accessioni | P27809Primary (citable) accession number: P27809 Secondary accession number(s): D6VTA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families