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Protein

3-ketoacyl-CoA thiolase, peroxisomal

Gene

POT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125Acyl-thioester intermediate1
Active sitei375Proton acceptor1
Active sitei403Proton acceptor1

GO - Molecular functioni

  • acetyl-CoA C-acyltransferase activity Source: SGD
  • mRNA binding Source: SGD

GO - Biological processi

  • fatty acid beta-oxidation Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:YIL160C-MONOMER
YEAST:YIL160C-MONOMER
BRENDAi2.3.1.16 984
ReactomeiR-SCE-2046106 alpha-linolenic acid (ALA) metabolism
R-SCE-390247 Beta-oxidation of very long chain fatty acids
R-SCE-6798695 Neutrophil degranulation
R-SCE-9033241 Peroxisomal protein import
UniPathwayiUPA00199

Chemistry databases

SwissLipidsiSLP:000001408

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase, peroxisomal (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase
Beta-ketothiolase
Peroxisomal 3-oxoacyl-CoA thiolase
Gene namesi
Name:POT1
Synonyms:FOX3, POX3
Ordered Locus Names:YIL160C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL160C
SGDiS000001422 POT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000034078? – 4173-ketoacyl-CoA thiolase, peroxisomal
Transit peptidei1 – ?Peroxisome

Proteomic databases

PaxDbiP27796
PRIDEiP27796

2D gel databases

UCD-2DPAGEiP27796

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34832, 274 interactors
DIPiDIP-1504N
IntActiP27796, 10 interactors
MINTiP27796
STRINGi4932.YIL160C

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Turni26 – 29Combined sources4
Beta strandi36 – 43Combined sources8
Turni49 – 51Combined sources3
Turni53 – 56Combined sources4
Helixi59 – 72Combined sources14
Helixi76 – 79Combined sources4
Helixi82 – 84Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi93 – 96Combined sources4
Helixi97 – 99Combined sources3
Helixi100 – 109Combined sources10
Beta strandi118 – 122Combined sources5
Helixi124 – 126Combined sources3
Helixi127 – 140Combined sources14
Beta strandi145 – 154Combined sources10
Helixi155 – 158Combined sources4
Helixi159 – 161Combined sources3
Helixi171 – 175Combined sources5
Helixi179 – 183Combined sources5
Helixi186 – 196Combined sources11
Helixi201 – 220Combined sources20
Turni225 – 227Combined sources3
Helixi251 – 255Combined sources5
Turni263 – 265Combined sources3
Turni270 – 272Combined sources3
Beta strandi276 – 287Combined sources12
Helixi288 – 293Combined sources6
Beta strandi300 – 309Combined sources10
Helixi312 – 317Combined sources6
Helixi318 – 330Combined sources13
Helixi334 – 336Combined sources3
Beta strandi338 – 342Combined sources5
Helixi347 – 357Combined sources11
Helixi361 – 363Combined sources3
Helixi370 – 373Combined sources4
Turni377 – 379Combined sources3
Helixi380 – 391Combined sources12
Beta strandi397 – 404Combined sources8
Turni405 – 407Combined sources3
Beta strandi408 – 416Combined sources9

3D structure databases

ProteinModelPortaliP27796
SMRiP27796
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27796

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00910000144143
HOGENOMiHOG000012239
InParanoidiP27796
KOiK07513
OMAiICPSIAI
OrthoDBiEOG092C2K2G

Family and domain databases

CDDicd00751 thiolase, 1 hit
Gene3Di3.40.47.10, 4 hits
InterProiView protein in InterPro
IPR002155 Thiolase
IPR016039 Thiolase-like
IPR020615 Thiolase_acyl_enz_int_AS
IPR020610 Thiolase_AS
IPR020617 Thiolase_C
IPR020613 Thiolase_CS
IPR020616 Thiolase_N
PfamiView protein in Pfam
PF02803 Thiolase_C, 1 hit
PF00108 Thiolase_N, 1 hit
PIRSFiPIRSF000429 Ac-CoA_Ac_transf, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits
TIGRFAMsiTIGR01930 AcCoA-C-Actrans, 1 hit
PROSITEiView protein in PROSITE
PS00098 THIOLASE_1, 1 hit
PS00737 THIOLASE_2, 1 hit
PS00099 THIOLASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27796-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRLQSIKD HLVESAMGKG ESKRKNSLLE KRPEDVVIVA ANRSAIGKGF
60 70 80 90 100
KGAFKDVNTD YLLYNFLNEF IGRFPEPLRA DLNLIEEVAC GNVLNVGAGA
110 120 130 140 150
TEHRAACLAS GIPYSTPFVA LNRQCSSGLT AVNDIANKIK VGQIDIGLAL
160 170 180 190 200
GVESMTNNYK NVNPLGMISS EELQKNREAK KCLIPMGITN ENVAANFKIS
210 220 230 240 250
RKDQDEFAAN SYQKAYKAKN EGLFEDEILP IKLPDGSICQ SDEGPRPNVT
260 270 280 290 300
AESLSSIRPA FIKDRGTTTA GNASQVSDGV AGVLLARRSV ANQLNLPVLG
310 320 330 340 350
RYIDFQTVGV PPEIMGVGPA YAIPKVLEAT GLQVQDIDIF EINEAFAAQA
360 370 380 390 400
LYCIHKLGID LNKVNPRGGA IALGHPLGCT GARQVATILR ELKKDQIGVV
410
SMCIGTGMGA AAIFIKE
Length:417
Mass (Da):44,730
Last modified:August 1, 1992 - v1
Checksum:iB22AA06130829DE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA Translation: CAA86118.1
X53946 Genomic DNA Translation: CAA37893.1
X53395 Genomic DNA Translation: CAA37472.1
AY693184 Genomic DNA Translation: AAT93203.1
BK006942 Genomic DNA Translation: DAA08392.1
PIRiS22784
RefSeqiNP_012106.1, NM_001179508.1

Genome annotation databases

EnsemblFungiiYIL160C; YIL160C; YIL160C
GeneIDi854646
KEGGisce:YIL160C

Similar proteinsi

Entry informationi

Entry nameiTHIK_YEAST
AccessioniPrimary (citable) accession number: P27796
Secondary accession number(s): D6VVC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 18, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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