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Protein

Protein disulfide-isomerase A3

Gene

Pdia3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Caution

Was originally thought to be a phosphatidylinositol 4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei57NucleophileBy similarity1
Sitei58Contributes to redox potential valueBy similarity1
Sitei59Contributes to redox potential valueBy similarity1
Active sitei60NucleophileBy similarity1
Sitei119Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei406NucleophileBy similarity1
Sitei407Contributes to redox potential valueBy similarity1
Sitei408Contributes to redox potential valueBy similarity1
Active sitei409NucleophileBy similarity1
Sitei471Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BRENDAi5.3.4.1 3474
ReactomeiR-MMU-1236974 ER-Phagosome pathway
R-MMU-901042 Calnexin/calreticulin cycle
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A3 (EC:5.3.4.1)
Alternative name(s):
58 kDa glucose-regulated protein
58 kDa microsomal protein
Short name:
p58
Disulfide isomerase ER-60
Endoplasmic reticulum resident protein 57
Short name:
ER protein 57
Short name:
ERp57
Endoplasmic reticulum resident protein 60
Short name:
ER protein 60
Short name:
ERp60
Gene namesi
Name:Pdia3
Synonyms:Erp, Erp60, Grp58
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95834 Pdia3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 242 PublicationsAdd BLAST24
ChainiPRO_000003422625 – 505Protein disulfide-isomerase A3Add BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 60Redox-activePROSITE-ProRule annotation
Disulfide bondi57Interchain (with C-118 in TAPBP); in linked formBy similarity
Modified residuei61N6-methyllysineBy similarity1
Disulfide bondi85 ↔ 92By similarity
Modified residuei129N6-succinyllysineCombined sources1
Modified residuei152N6-acetyllysineCombined sources1
Modified residuei218N6-succinyllysineCombined sources1
Modified residuei252N6-acetyllysineCombined sources1
Modified residuei319PhosphothreonineBy similarity1
Modified residuei362N6-acetyllysineCombined sources1
Disulfide bondi406 ↔ 409Redox-activePROSITE-ProRule annotation
Modified residuei494N6-acetyllysineCombined sources1

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Acetylation, Disulfide bond, Methylation, Phosphoprotein

Proteomic databases

EPDiP27773
MaxQBiP27773
PaxDbiP27773
PeptideAtlasiP27773
PRIDEiP27773
TopDownProteomicsiP27773

2D gel databases

REPRODUCTION-2DPAGEiIPI00230108
P27773
Q3TEI9
Q8C2F4
SWISS-2DPAGEiP27773

PTM databases

iPTMnetiP27773
PhosphoSitePlusiP27773
SwissPalmiP27773

Expressioni

Tissue specificityi

In caput and cauda epididymal spermatozoa, detected in the acrosome and principal piece (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027248 Expressed in 288 organ(s), highest expression level in pituitary gland
CleanExiMM_PDIA3
ExpressionAtlasiP27773 baseline and differential
GenevisibleiP27773 MM

Interactioni

Subunit structurei

Subunit of the TAP complex, also known as the peptide loading complex (PLC). Can form disulfide-linked heterodimers with TAPBP. Interacts with ERP27 and CANX (By similarity). Interacts with MZB1 in a calcium-dependent manner. Interacts with SERPINA2 and with the S and Z variants of SERPINA1 (By similarity). Interacts with ATP2A2 (PubMed:23395171).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200077, 4 interactors
ComplexPortaliCPX-531 Tapasin-ERp57 complex
IntActiP27773, 9 interactors
MINTiP27773
STRINGi10090.ENSMUSP00000028683

Structurei

3D structure databases

ProteinModelPortaliP27773
SMRiP27773
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 133Thioredoxin 1PROSITE-ProRule annotationAdd BLAST109
Domaini343 – 485Thioredoxin 2PROSITE-ProRule annotationAdd BLAST143

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi502 – 505Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00930000150840
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP27773
KOiK08056
OrthoDBiEOG091G05J9
PhylomeDBiP27773
TreeFamiTF106382

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P27773-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRFSCLALLP GVALLLASAR LAAASDVLEL TDENFESRVS DTGSAGLMLV
60 70 80 90 100
EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY
110 120 130 140 150
PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS
160 170 180 190 200
DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG
210 220 230 240 250
EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED
260 270 280 290 300
NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA
310 320 330 340 350
VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE
360 370 380 390 400
QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE
410 420 430 440 450
FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF
460 470 480 490 500
PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK

AQEDL
Length:505
Mass (Da):56,678
Last modified:May 1, 2007 - v2
Checksum:i3A7CD1C35981C4B3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q404F6Q404_MOUSE
Protein disulfide-isomerase A3
Pdia3
124Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20R → C in BAC40527 (PubMed:16141072).Curated1
Sequence conflicti20R → C in BAE41259 (PubMed:16141072).Curated1
Sequence conflicti76Missing in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti108D → A in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti230G → A in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti264Y → F in BAE39834 (PubMed:16141072).Curated1
Sequence conflicti315G → S in AAA39944 (PubMed:2033248).Curated1
Sequence conflicti387D → G in BAE39834 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73329 mRNA Translation: AAA39944.1
DQ000491 Genomic DNA Translation: AAY16987.1
AK088721 mRNA Translation: BAC40527.1
AK133799 mRNA Translation: BAE21849.1
AK167807 mRNA Translation: BAE39834.1
AK169611 mRNA Translation: BAE41259.1
AL845466 Genomic DNA Translation: CAM24231.1
BC003285 mRNA Translation: AAH03285.1
BC033439 mRNA Translation: AAH33439.1
CCDSiCCDS16643.1
RefSeqiNP_031978.2, NM_007952.2
UniGeneiMm.263177

Genome annotation databases

EnsembliENSMUST00000028683; ENSMUSP00000028683; ENSMUSG00000027248
GeneIDi14827
KEGGimmu:14827
UCSCiuc008lzb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73329 mRNA Translation: AAA39944.1
DQ000491 Genomic DNA Translation: AAY16987.1
AK088721 mRNA Translation: BAC40527.1
AK133799 mRNA Translation: BAE21849.1
AK167807 mRNA Translation: BAE39834.1
AK169611 mRNA Translation: BAE41259.1
AL845466 Genomic DNA Translation: CAM24231.1
BC003285 mRNA Translation: AAH03285.1
BC033439 mRNA Translation: AAH33439.1
CCDSiCCDS16643.1
RefSeqiNP_031978.2, NM_007952.2
UniGeneiMm.263177

3D structure databases

ProteinModelPortaliP27773
SMRiP27773
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200077, 4 interactors
ComplexPortaliCPX-531 Tapasin-ERp57 complex
IntActiP27773, 9 interactors
MINTiP27773
STRINGi10090.ENSMUSP00000028683

PTM databases

iPTMnetiP27773
PhosphoSitePlusiP27773
SwissPalmiP27773

2D gel databases

REPRODUCTION-2DPAGEiIPI00230108
P27773
Q3TEI9
Q8C2F4
SWISS-2DPAGEiP27773

Proteomic databases

EPDiP27773
MaxQBiP27773
PaxDbiP27773
PeptideAtlasiP27773
PRIDEiP27773
TopDownProteomicsiP27773

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028683; ENSMUSP00000028683; ENSMUSG00000027248
GeneIDi14827
KEGGimmu:14827
UCSCiuc008lzb.1 mouse

Organism-specific databases

CTDi2923
MGIiMGI:95834 Pdia3

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA
GeneTreeiENSGT00930000150840
HOGENOMiHOG000162459
HOVERGENiHBG005920
InParanoidiP27773
KOiK08056
OrthoDBiEOG091G05J9
PhylomeDBiP27773
TreeFamiTF106382

Enzyme and pathway databases

BRENDAi5.3.4.1 3474
ReactomeiR-MMU-1236974 ER-Phagosome pathway
R-MMU-901042 Calnexin/calreticulin cycle
R-MMU-983170 Antigen Presentation: Folding, assembly and peptide loading of class I MHC

Miscellaneous databases

ChiTaRSiPdia3 mouse
PROiPR:P27773
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027248 Expressed in 288 organ(s), highest expression level in pituitary gland
CleanExiMM_PDIA3
ExpressionAtlasiP27773 baseline and differential
GenevisibleiP27773 MM

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA3_MOUSE
AccessioniPrimary (citable) accession number: P27773
Secondary accession number(s): Q3TEI9
, Q3TIL2, Q3UZK8, Q8C2F4, Q99LF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 179 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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