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Protein

N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase

Gene

acvA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Each of the constituent amino acids of the tripeptide acv are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates.

Catalytic activityi

L-2-aminohexanedioate + L-cysteine + L-valine + 3 ATP + H2O = N-(L-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + 3 AMP + 3 diphosphate.

Cofactori

pantetheine 4'-phosphateCuratedNote: Binds 3 phosphopantetheines covalently.Curated

Pathwayi: penicillin G biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (acvA)
  2. Isopenicillin N synthase (ipnA)
  3. Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (penDE)
This subpathway is part of the pathway penicillin G biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine, the pathway penicillin G biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3623For thioesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • penicillin biosynthetic process Source: AspGD
  • secondary metabolic process Source: AspGD

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processAntibiotic biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00149; UER00239

Protein family/group databases

ESTHERiemeni-acvs Thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (EC:6.3.2.26)
Alternative name(s):
Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase
Short name:
ACV synthetase
Short name:
ACVS
Gene namesi
Name:acvA
ORF Names:AN2621
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VI
  • UP000005890 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001930571 – 3770N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthaseAdd BLAST3770

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi309N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi570N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi596N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi638N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi639N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi655N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi787N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei882O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Glycosylationi1256N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1402N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1545N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1735N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1965O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Glycosylationi2410N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei3050O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Glycosylationi3130N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi3298N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi3332N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The N-terminus is blocked.
Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphopantetheine, Phosphoprotein

Proteomic databases

PRIDEiP27742

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00010505

Structurei

3D structure databases

ProteinModelPortaliP27742
SMRiP27742
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini845 – 922Carrier 1PROSITE-ProRule annotationAdd BLAST78
Domaini1928 – 2005Carrier 2PROSITE-ProRule annotationAdd BLAST78
Domaini3015 – 3090Carrier 3PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni321 – 910Domain 1 (adipate-activating)Add BLAST590
Regioni1413 – 1993Domain 2 (cysteine-activating)Add BLAST581
Regioni2494 – 3078Domain 3 (valine-activating)Add BLAST585

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000033796
InParanoidiP27742
KOiK12743
OMAiNRQHWNQ
OrthoDBiEOG092C0041

Family and domain databases

Gene3Di1.10.1200.10, 3 hits
3.30.559.10, 5 hits
3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR029058 AB_hydrolase
IPR036736 ACP-like_sf
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR023213 CAT-like_dom_sf
IPR001242 Condensatn
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR006162 Ppantetheine_attach_site
IPR001031 Thioesterase
PfamiView protein in Pfam
PF00501 AMP-binding, 3 hits
PF13193 AMP-binding_C, 2 hits
PF00668 Condensation, 3 hits
PF00550 PP-binding, 3 hits
PF00975 Thioesterase, 1 hit
SMARTiView protein in SMART
SM00823 PKS_PP, 3 hits
SUPFAMiSSF47336 SSF47336, 3 hits
SSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 3 hits
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 3 hits
PS50075 CARRIER, 3 hits
PS00012 PHOSPHOPANTETHEINE, 3 hits

Sequencei

Sequence statusi: Complete.

P27742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPGLLSED GPGYSGGYAD PTVPKVNWKQ SNGKSAGGNG DVDAGNGNID
60 70 80 90 100
PSKSGVGVQV CFAGGLEGWK AGISKITERC DLSSIATNST KYQLAVTGFS
110 120 130 140 150
DGPDDYNEYS VPFPSEVLVA MEEMCLARDI SMRSVIQFAV HYVLKGFGGG
160 170 180 190 200
SHTVAASIDV GDDPNNIATS YTITPSIVCH ESRQGQTVMQ EIQSMEKLNQ
210 220 230 240 250
LRKQEMHPGE AGLSLIRMGL FDILVIFADA NKCEGLIAGL PLAVMVCEGG
260 270 280 290 300
GRLQVRIHFS GSLFRQKTLV DIAEALNVLF AKAASGGATP VRDLELLSAE
310 320 330 340 350
QKQQLEEWNK TDGEYPECKR LNHLIEEATQ LHEDKVAIVY KRRQLTYGEL
360 370 380 390 400
NAQANCFAHY LRSIGILPEQ LVALFLEKSE NLIVTILGIW KSGAAYVPID
410 420 430 440 450
PTYPDERVRF VLEDTQAKVI IASNHLAERL QSEVISDREL SIIRLEHCLS
460 470 480 490 500
AIDQQPSTFP RANLRDPSLT SKQLAYVTYT SGTTGFPKGI LKQHTNVVNS
510 520 530 540 550
ITDLSARYGV TGDHHEAILL FSAYVFEPFV RQMLMALVNG HLLAMVDDAE
560 570 580 590 600
KYDAEKLIPF IREHKITYLN GTASVLQEYD FSSCPSLKRL ILVGENLTES
610 620 630 640 650
RYLALRRHFK NCILNEYGFT ESAFVTALNV FEPGSARNNT SLGRPVRNVK
660 670 680 690 700
CYILNKSLKR VPIGATGELH IGGLGISKGY LNRPDLTPQR FIPNPFQTDH
710 720 730 740 750
EKELGLNQLM YKTGDLARWL PNGEIEYLGR ADFQIKLRGI RIEPGEIEST
760 770 780 790 800
LAGYPGVRTS LVVSKRLRHG EKETTNEHLV GYYVGDNTSV SETALLQFLE
810 820 830 840 850
LKLPRYMIPT RLVRVSQIPV TVNGKADLRA LPSVDLIQPK VSSCELTDEV
860 870 880 890 900
EIALGKIWAD VLGAHHLSIS RKDNFFRLGG HSITCIQLIA RIRQQLGVII
910 920 930 940 950
SIEDVFSSRT LERMAELLRS KESNGTPDER ARPQLKTVAG EVANANVYLA
960 970 980 990 1000
NSLQQGFVYQ FLKNMGRSEA YVMQSVLRYD VNINPDLFKK AWKQVQHMLP
1010 1020 1030 1040 1050
TLRLRFQWGQ DVLQVIDEDQ PLNWWFLHLA DDSALPEEQK LLELQRRDLA
1060 1070 1080 1090 1100
EPYDLAAGSL FRIYLIEHSS TRFSCLFSCH HAILDGWSLP LLFRKTHGTY
1110 1120 1130 1140 1150
LHLLHGHSLR TLEDPYRQSQ QYLQDHREDH LRYWAGIVNQ IEERCDMNAL
1160 1170 1180 1190 1200
LNERSRYKIQ LADYDKVEDQ QQLTLTVPDA SWLSKLRQTC SAQGITLHSI
1210 1220 1230 1240 1250
LQFVWHAVLH AYGGGTHTVT GTTISGRNLP VSGIERSVGL YINTLPLVIN
1260 1270 1280 1290 1300
QLAYKNKTVL EAIRDVQAIV NGMNSRGNVE LGRLQKNELK HGLFDSLFVL
1310 1320 1330 1340 1350
ENYPILDKSE EMRQKSELKY TIEGNIEKLD YPLAVIAREV DLTGGFTFTI
1360 1370 1380 1390 1400
CYARELFDEI VISELLQMVR DTLLQVAKHL DDPVRSLEYL SSAQMAQLDA
1410 1420 1430 1440 1450
WNATDAEFPD TTLHAMFEKE AAQKPDKVAV VYEQRSLTYR QLNERANRMA
1460 1470 1480 1490 1500
HQLKSDISPK PNSIIALVVD KSEHMIATIL AVWKTGGAYV PIDPEYPDDR
1510 1520 1530 1540 1550
IRYILEDTSA IAVISDACYL SRIQELAGES VRLYRSDIST QTDGNWSVSN
1560 1570 1580 1590 1600
PAPSSTSTDL AYIIYTSGTT GKPKGVMVEH HGVVNLQISL SKTFGLRDTD
1610 1620 1630 1640 1650
DEVILSFSNY VFDHFVEQMT DAILNGQTLV MLNDAMRSDK ERLYQYIETN
1660 1670 1680 1690 1700
RVTYLSGTPS VISMYEFSRF KDHLRRVDCV GEAFSQPVFD QIRDTFQGLI
1710 1720 1730 1740 1750
INGYGPTEIS ITTHKRLYPF PERRTDKSIG QQIGNSTSYV LNADMKRVPI
1760 1770 1780 1790 1800
GAVGELYLGG EGVARGYHNR PEVTAERFLR NPFQTDSERQ NGRNSRLYRT
1810 1820 1830 1840 1850
GDLVRWIPGS NGEIEYLGRN DFQVKIRGLR IELGEIEAVM SSHPDIKQSV
1860 1870 1880 1890 1900
VIAKSGKEGD QKFLVGYFVA SSPLSPGAIR RFMQSRLPGY MIPSSFIPIS
1910 1920 1930 1940 1950
SLPVTPSGKL DTKALPTAEE KGAMNVLAPR NEIESILCGI WAGLLDISAQ
1960 1970 1980 1990 2000
TIGSDSDFFT LGGDSLKSTK LSFKIHEVFG RTISVSALFR HRTIESLAHL
2010 2020 2030 2040 2050
IMNNVGDIQE ITPVDYDNRR KIAVSPAQER LLFIHELEGG GNAYNIDAAF
2060 2070 2080 2090 2100
ELPPYIDQSR VEEALYTILS RHEALRTFLL RDQATGTFYQ KILTTDEAKC
2110 2120 2130 2140 2150
MLIIEKSAVS TIDQIDSIVG RLSQHIFRLD SELPWLAHIV THKTGNLYLT
2160 2170 2180 2190 2200
LSFHHTCFDA WSLKIFEREL RVFCASNEKG GNMPILPMPQ VQYKEYAEHH
2210 2220 2230 2240 2250
RRRLGKNQIQ KLSDFWLQRL DGLEPLQLLP DYPRPAQFNY DGGDLSVILD
2260 2270 2280 2290 2300
GVVLETLRGI AKDHGVTLYA VLLAVYCLML STYTHQVDIA VGVPISHRTH
2310 2320 2330 2340 2350
PLFQSIVGFF VNMVVVRVDV KDFAVHDLIR RVMKALVDAQ LHQDMPFQDV
2360 2370 2380 2390 2400
TKLLRVDNDA SRHPLVQTVF NFESDMDKEF ETTPSIQDTA TIAPYQSVQR
2410 2420 2430 2440 2450
IKSVAKFDLN ATATESGSAL KINFNYATSL FRKETIQGFL ETYRHLLLQL
2460 2470 2480 2490 2500
SYLGSQGLKE DTKLLLVRPE EMSGPHLPLA GLSNGAETLE AISLSRAFEF
2510 2520 2530 2540 2550
EAFRVPDRAA VVQGDKSLSY TELNKRANQL ARYIQSVAHL RPDDKVLLIL
2560 2570 2580 2590 2600
DKSIDMIICI LAIWKTGSAY VPLDPSYPKE RVQCISEVVQ AKILITESRY
2610 2620 2630 2640 2650
ASAWGSQTST ILAIDSPKVS NMVNNQATHN LPNIAGIKNL AYIIFTSGTS
2660 2670 2680 2690 2700
GKPKGVLVEQ GGVLHLRDAL RKRYFGIECN EYHAVLFLSN YVFDFSIEQL
2710 2720 2730 2740 2750
VLSIMSGHKL IIPEGEFVAD DEFYITANGQ RLSYLSGTPS LLQQIDLARL
2760 2770 2780 2790 2800
NHLQVVTAAG EQLHAAQFNK LRSGFRGPIY NAYGITETTV YNIVSEFSAQ
2810 2820 2830 2840 2850
SQFENALREL LPGTRAYLLN HATQPVPMNA VGELYLAGDC VARGYLNQPV
2860 2870 2880 2890 2900
LTGDRFIQNP FQTEQDIASG SYPRLYRTGD LFRCRLDRQH QPYLEYLGRA
2910 2920 2930 2940 2950
DLQVKIRGYR IEPSEVQNVL ASCPGVRECA VVAKYENTDA YSRIAKFLVG
2960 2970 2980 2990 3000
YYTPDTETVS DSSILAHMKS KLPAYMVPKY LCRLEGGLPV TINGKLDVRK
3010 3020 3030 3040 3050
LPDIGNPQHQ ISYNPPRDVL EADLCRLWAS ALGTERCGID DDLFRLGGDS
3060 3070 3080 3090 3100
ITALHLAAQI HHQIGRKVTV RDIFDHPTIR GIHDNVMVKL VPHNVPQFQA
3110 3120 3130 3140 3150
EQQTVLGDAP LLPIQTWFLS KSLQHPSHWN HTFYLRTPEL DVTTLSTAVA
3160 3170 3180 3190 3200
ELQLYHDAFR MRLRQIDGRT VQCFADDISP VQLRVLNVKD VDGSAAIDQQ
3210 3220 3230 3240 3250
LQKYQSDFDL EKGPICAAAY LHGYEDRSAR VWFSVHHIII DIVSWQILAR
3260 3270 3280 3290 3300
DLQILYEGGT LGRKSSSVRQ WAEALQSYQG SASERAYWEG LLAQTAANIS
3310 3320 3330 3340 3350
ALPPVTGTRT RLARTWSDDR TVILLNEASN QNASIQDLLL AAVGLALQQV
3360 3370 3380 3390 3400
TPGSPSMITL EGHGREEIVD PTLDLSRTLG WFTSMYPFEI PPLNVETLSQ
3410 3420 3430 3440 3450
GIASLRECLR QVPARGIGFG SLYGYCKHQM PQVTFNYLGQ LTSKQSITDQ
3460 3470 3480 3490 3500
WALAVGDGEM QYGLTTSPAD RDQSSFAVDI TASCVNGALS VEMNSAWSLE
3510 3520 3530 3540 3550
KSMRFISRIE EVLNMILSGT LAQQATPVLT PQVFNEEMYT PYFEFSKTPR
3560 3570 3580 3590 3600
RGPILFLLPP GEGGAESYFN NIVKHLPTTN MVVFNNYYLH SKSLNTFEKL
3610 3620 3630 3640 3650
AEMYLGHIRQ IQPDGPYHFI GWSFGGTIAM EISRQLVGLG STIGLLGIID
3660 3670 3680 3690 3700
TYFNVPGATR AIGLGDTEVL DPIHHISQPE PADFQCLPAS TDYIILFKAT
3710 3720 3730 3740 3750
RVNDKFQSEN QRRLYEYYDK TLLNDLDWLL PGASNIHLVR LEEDTHFSWA
3760 3770
TNPRQIAHVC STIEKFLARY
Length:3,770
Mass (Da):422,502
Last modified:May 26, 2009 - v2
Checksum:iB31873DD4106D7B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1941W → S in AAA03914 (PubMed:2061333).Curated1
Sequence conflicti1941W → S in CAA38631 (PubMed:2061333).Curated1
Sequence conflicti1941W → S AA sequence (PubMed:2061333).Curated1
Sequence conflicti2177 – 2178NE → KQ in AAA03914 (PubMed:2061333).Curated2
Sequence conflicti2177 – 2178NE → KQ in CAA38631 (PubMed:2061333).Curated2
Sequence conflicti2335A → P in AAA03914 (PubMed:2061333).Curated1
Sequence conflicti2335A → P in CAA38631 (PubMed:2061333).Curated1
Sequence conflicti2869S → C in AAA03914 (PubMed:2061333).Curated1
Sequence conflicti2869S → C in CAA38631 (PubMed:2061333).Curated1
Sequence conflicti3116T → I in AAA03914 (PubMed:2061333).Curated1
Sequence conflicti3116T → I in CAA38631 (PubMed:2061333).Curated1
Sequence conflicti3116T → I AA sequence (PubMed:2061333).Curated1
Sequence conflicti3139E → D in CAA38631 (PubMed:2061333).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S40212 Genomic DNA Translation: AAA03914.1
X54853 Genomic DNA Translation: CAA38631.1
AACD01000045 Genomic DNA Translation: EAA62968.1
BN001306 Genomic DNA Translation: CBF84349.1
PIRiA40889
RefSeqiXP_660225.1, XM_655133.1

Genome annotation databases

EnsemblFungiiCBF84349; CBF84349; ANIA_02621
EAA62968; EAA62968; AN2621.2
GeneIDi2874260
KEGGiani:AN2621.2

Similar proteinsi

Entry informationi

Entry nameiACVS_EMENI
AccessioniPrimary (citable) accession number: P27742
Secondary accession number(s): C8VHS8, Q4J6A8, Q5BA09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: May 26, 2009
Last modified: June 20, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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