UniProtKB - P27661 (H2AX_MOUSE)
Histone H2AX
H2afx
Functioni
Miscellaneous
GO - Molecular functioni
- damaged DNA binding Source: MGI
- DNA binding Source: GO_Central
- enzyme binding Source: MGI
- histone binding Source: MGI
- protein heterodimerization activity Source: InterPro
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- cellular response to gamma radiation Source: MGI
- cellular senescence Source: Ensembl
- cerebral cortex development Source: Ensembl
- chromatin organization Source: GO_Central
- DNA repair Source: MGI
- double-strand break repair via homologous recombination Source: MGI
- meiotic cell cycle Source: UniProtKB-KW
- spermatogenesis Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, DNA damage, DNA recombination, DNA repair, Meiosis |
Enzyme and pathway databases
Reactomei | R-MMU-110330 Recognition and association of DNA glycosylase with site containing an affected purine R-MMU-110331 Cleavage of the damaged purine R-MMU-171306 Packaging Of Telomere Ends R-MMU-201722 Formation of the beta-catenin:TCF transactivating complex R-MMU-212300 PRC2 methylates histones and DNA R-MMU-2299718 Condensation of Prophase Chromosomes R-MMU-2559580 Oxidative Stress Induced Senescence R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP) R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence R-MMU-427359 SIRT1 negatively regulates rRNA expression R-MMU-427413 NoRC negatively regulates rRNA expression R-MMU-5250924 B-WICH complex positively regulates rRNA expression R-MMU-5578749 Transcriptional regulation by small RNAs R-MMU-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-MMU-5693571 Nonhomologous End-Joining (NHEJ) R-MMU-5693607 Processing of DNA double-strand break ends R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere R-MMU-69473 G2/M DNA damage checkpoint R-MMU-73728 RNA Polymerase I Promoter Opening R-MMU-73772 RNA Polymerase I Promoter Escape R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-9018519 Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H2AXShort name: H2a/x Alternative name(s): Histone H2A.X |
Gene namesi | Name:H2afx Synonyms:H2a.x, H2ax, Hist5-2ax |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102688 H2afx |
Subcellular locationi
Nucleus
- Nucleus 6 Publications
Other locations
- Chromosome 6 Publications
Cytoskeleton
- centrosome Source: UniProtKB
Nucleus
- condensed nuclear chromosome Source: MGI
- male germ cell nucleus Source: MGI
- nuclear chromatin Source: MGI
- nuclear speck Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- chromatin Source: MGI
- chromosome Source: UniProtKB
- chromosome, telomeric region Source: Ensembl
- nucleosome Source: UniProtKB-KW
- replication fork Source: MGI
- site of DNA damage Source: UniProtKB
- site of double-strand break Source: MGI
- XY body Source: MGI
Keywords - Cellular componenti
Chromosome, Nucleosome core, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 6 | K → A: No effect on radiosensitivity; when associated with A-10, A-14 and A-16. 1 Publication | 1 | |
Mutagenesisi | 10 | K → A: No effect on radiosensitivity; when associated with A-6, A-14 and A-16. 1 Publication | 1 | |
Mutagenesisi | 14 | K → A: No effect on radiosensitivity; when associated with A-6, A-10 and A-16. 1 Publication | 1 | |
Mutagenesisi | 16 | K → A: No effect on radiosensitivity; when associated with A-6, A-10 and A-14. 1 Publication | 1 | |
Mutagenesisi | 37 | K → A: Increased radiosensitivity. No effect on phosphorylation after DNA damage. No effect on Ser-140 phosphorylation, nor on TP53BP1 recruitment to DNA double-strand breaks. 1 Publication | 1 | |
Mutagenesisi | 37 | K → R: No effect on phosphorylation after DNA damage, but increased radiosensitivity. Further increase in radiosensitivity; when associated with A-140. 1 Publication | 1 | |
Mutagenesisi | 119 – 120 | KK → AA: Complete loss of ubiquitination and increased radiosensitivity. 1 Publication | 2 | |
Mutagenesisi | 137 | S → A: Increased genomic instability and radiosensitivity; when associated with A-140. 1 Publication | 1 | |
Mutagenesisi | 140 | S → A: Increased genomic instability and radiosensitivity; when associated with A-137. Reduced homologous recombination. No effect on Lys-40 acetylation. Further increase in radiosensitivity; when associated with R-37. 3 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000055243 | 2 – 143 | Histone H2AXAdd BLAST | 142 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 | |
Modified residuei | 2 | Phosphoserine1 Publication | 1 | |
Modified residuei | 6 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 10 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 14 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 16 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 37 | N6-acetyllysine; by CREBBP and EP3001 Publication | 1 | |
Cross-linki | 120 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 121 | PhosphoserineCombined sources | 1 | |
Modified residuei | 122 | PhosphoserineCombined sources | 1 | |
Cross-linki | 128 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 135 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 137 | PhosphoserineCombined sources | 1 | |
Modified residuei | 140 | Phosphoserine; by ATM, ATR and PRKDCCombined sources9 Publications | 1 | |
Modified residuei | 143 | Phosphotyrosine; by WSTF1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P27661 |
jPOSTi | P27661 |
MaxQBi | P27661 |
PaxDbi | P27661 |
PeptideAtlasi | P27661 |
PRIDEi | P27661 |
TopDownProteomicsi | P27661 |
PTM databases
iPTMneti | P27661 |
PhosphoSitePlusi | P27661 |
SwissPalmi | P27661 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000049932 Expressed in 288 organ(s), highest expression level in neocortex |
Genevisiblei | P27661 MM |
Interactioni
Subunit structurei
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN.
Also interacts with DHX9/NDHII when phosphorylated on Ser-140 and MCPH1 when phosphorylated at Ser-140 or Tyr-143.
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.
Interacts with WRAP53/TCAB1.
By similarityBinary interactionsi
GO - Molecular functioni
- enzyme binding Source: MGI
- histone binding Source: MGI
- protein heterodimerization activity Source: InterPro
Protein-protein interaction databases
BioGridi | 200313, 16 interactors |
IntActi | P27661, 13 interactors |
MINTi | P27661 |
STRINGi | 10090.ENSMUSP00000051432 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 140 – 141 | [ST]-Q motif | 2 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1756 Eukaryota COG5262 LUCA |
GeneTreei | ENSGT00950000182829 |
HOGENOMi | HOG000234652 |
InParanoidi | P27661 |
KOi | K11251 |
OMAi | RNDEELX |
OrthoDBi | 1504122at2759 |
PhylomeDBi | P27661 |
TreeFami | TF300137 |
Family and domain databases
CDDi | cd00074 H2A, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR009072 Histone-fold IPR002119 Histone_H2A IPR007125 Histone_H2A/H2B/H3 IPR032454 Histone_H2A_C IPR032458 Histone_H2A_CS |
Pfami | View protein in Pfam PF00125 Histone, 1 hit PF16211 Histone_H2A_C, 1 hit |
PRINTSi | PR00620 HISTONEH2A |
SMARTi | View protein in SMART SM00414 H2A, 1 hit |
SUPFAMi | SSF47113 SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00046 HISTONE_H2A, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSGRGKTGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGHY AERVGAGAPV
60 70 80 90 100
YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGG
110 120 130 140
VTIAQGGVLP NIQAVLLPKK SSATVGPKAP AVGKKASQAS QEY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X58069 mRNA Translation: CAA41099.1 Z35401 Genomic DNA Translation: CAA84585.1 BC005468 mRNA Translation: AAH05468.1 BC010336 mRNA Translation: AAH10336.1 |
CCDSi | CCDS23105.1 |
PIRi | I48406 |
RefSeqi | NP_034566.1, NM_010436.2 |
Genome annotation databases
Ensembli | ENSMUST00000052686; ENSMUSP00000051432; ENSMUSG00000049932 |
GeneIDi | 15270 |
KEGGi | mmu:15270 |
UCSCi | uc009pcy.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X58069 mRNA Translation: CAA41099.1 Z35401 Genomic DNA Translation: CAA84585.1 BC005468 mRNA Translation: AAH05468.1 BC010336 mRNA Translation: AAH10336.1 |
CCDSi | CCDS23105.1 |
PIRi | I48406 |
RefSeqi | NP_034566.1, NM_010436.2 |
3D structure databases
SMRi | P27661 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 200313, 16 interactors |
IntActi | P27661, 13 interactors |
MINTi | P27661 |
STRINGi | 10090.ENSMUSP00000051432 |
PTM databases
iPTMneti | P27661 |
PhosphoSitePlusi | P27661 |
SwissPalmi | P27661 |
Proteomic databases
EPDi | P27661 |
jPOSTi | P27661 |
MaxQBi | P27661 |
PaxDbi | P27661 |
PeptideAtlasi | P27661 |
PRIDEi | P27661 |
TopDownProteomicsi | P27661 |
Genome annotation databases
Ensembli | ENSMUST00000052686; ENSMUSP00000051432; ENSMUSG00000049932 |
GeneIDi | 15270 |
KEGGi | mmu:15270 |
UCSCi | uc009pcy.1 mouse |
Organism-specific databases
CTDi | 15270 |
MGIi | MGI:102688 H2afx |
Phylogenomic databases
eggNOGi | KOG1756 Eukaryota COG5262 LUCA |
GeneTreei | ENSGT00950000182829 |
HOGENOMi | HOG000234652 |
InParanoidi | P27661 |
KOi | K11251 |
OMAi | RNDEELX |
OrthoDBi | 1504122at2759 |
PhylomeDBi | P27661 |
TreeFami | TF300137 |
Enzyme and pathway databases
Reactomei | R-MMU-110330 Recognition and association of DNA glycosylase with site containing an affected purine R-MMU-110331 Cleavage of the damaged purine R-MMU-171306 Packaging Of Telomere Ends R-MMU-201722 Formation of the beta-catenin:TCF transactivating complex R-MMU-212300 PRC2 methylates histones and DNA R-MMU-2299718 Condensation of Prophase Chromosomes R-MMU-2559580 Oxidative Stress Induced Senescence R-MMU-2559582 Senescence-Associated Secretory Phenotype (SASP) R-MMU-2559586 DNA Damage/Telomere Stress Induced Senescence R-MMU-427359 SIRT1 negatively regulates rRNA expression R-MMU-427413 NoRC negatively regulates rRNA expression R-MMU-5250924 B-WICH complex positively regulates rRNA expression R-MMU-5578749 Transcriptional regulation by small RNAs R-MMU-5625886 Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-MMU-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-MMU-5693571 Nonhomologous End-Joining (NHEJ) R-MMU-5693607 Processing of DNA double-strand break ends R-MMU-606279 Deposition of new CENPA-containing nucleosomes at the centromere R-MMU-69473 G2/M DNA damage checkpoint R-MMU-73728 RNA Polymerase I Promoter Opening R-MMU-73772 RNA Polymerase I Promoter Escape R-MMU-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-MMU-9018519 Estrogen-dependent gene expression |
Miscellaneous databases
ChiTaRSi | H2afx mouse |
PROi | PR:P27661 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000049932 Expressed in 288 organ(s), highest expression level in neocortex |
Genevisiblei | P27661 MM |
Family and domain databases
CDDi | cd00074 H2A, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR009072 Histone-fold IPR002119 Histone_H2A IPR007125 Histone_H2A/H2B/H3 IPR032454 Histone_H2A_C IPR032458 Histone_H2A_CS |
Pfami | View protein in Pfam PF00125 Histone, 1 hit PF16211 Histone_H2A_C, 1 hit |
PRINTSi | PR00620 HISTONEH2A |
SMARTi | View protein in SMART SM00414 H2A, 1 hit |
SUPFAMi | SSF47113 SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00046 HISTONE_H2A, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H2AX_MOUSE | |
Accessioni | P27661Primary (citable) accession number: P27661 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | November 13, 2019 | |
This is version 191 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot