UniProtKB - P27659 (RL3_MOUSE)
Protein
60S ribosomal protein L3
Gene
Rpl3
Organism
Mus musculus (Mouse)
Status
Functioni
The L3 protein is a component of the large subunit of cytoplasmic ribosomes.By similarity
GO - Molecular functioni
- 5S rRNA binding Source: MGI
- RNA binding Source: GO_Central
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- cellular response to interleukin-4 Source: MGI
- ribosomal large subunit assembly Source: GO_Central
- translation Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L3Alternative name(s): J1 protein |
Gene namesi | Name:Rpl3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351605, Rpl3 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
- cytosolic large ribosomal subunit Source: MGI
Nucleus
- nucleolus Source: UniProtKB
Other locations
- protein-containing complex Source: MGI
- ribosome Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000077229 | 1 – 403 | 60S ribosomal protein L3Add BLAST | 403 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphoserineCombined sources | 1 | |
Cross-linki | 39 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 136 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 224 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 226 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 286 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 286 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 294 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 294 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Modified residuei | 304 | PhosphoserineBy similarity | 1 | |
Modified residuei | 366 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 366 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 373 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 386 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 393 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 399 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P27659 |
jPOSTi | P27659 |
MaxQBi | P27659 |
PaxDbi | P27659 |
PeptideAtlasi | P27659 |
PRIDEi | P27659 |
ProteomicsDBi | 299902 |
PTM databases
iPTMneti | P27659 |
PhosphoSitePlusi | P27659 |
SwissPalmi | P27659 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000060036, Expressed in ventricular zone and 89 other tissues |
ExpressionAtlasi | P27659, baseline and differential |
Genevisiblei | P27659, MM |
Interactioni
Subunit structurei
Component of the large ribosomal subunit.
Interacts with METTL18.
Interacts with DHX33 (PubMed:26100019).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 205174, 85 interactors |
ComplexPortali | CPX-5262, 60S cytosolic large ribosomal subunit |
CORUMi | P27659 |
IntActi | P27659, 7 interactors |
MINTi | P27659 |
STRINGi | 10090.ENSMUSP00000080354 |
Miscellaneous databases
RNActi | P27659, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the universal ribosomal protein uL3 family.Curated
Phylogenomic databases
eggNOGi | KOG0746, Eukaryota |
GeneTreei | ENSGT00390000017606 |
HOGENOMi | CLU_033361_2_1_1 |
InParanoidi | P27659 |
OMAi | HQRTEYN |
OrthoDBi | 793191at2759 |
TreeFami | TF300555 |
Family and domain databases
InterProi | View protein in InterPro IPR000597, Ribosomal_L3 IPR019926, Ribosomal_L3_CS IPR009000, Transl_B-barrel_sf |
Pfami | View protein in Pfam PF00297, Ribosomal_L3, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit |
PROSITEi | View protein in PROSITE PS00474, RIBOSOMAL_L3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P27659-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDASKP VHLTAFLGYK
60 70 80 90 100
AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGIV GYVETPRGLR
110 120 130 140 150
TFKTVFAEHI SDECKRRFYK NWHKSKKKAF TKYCKKWQDD TGKKQLEKDF
160 170 180 190 200
NSMKKYCQVI RIIAHTQMRL LPLRQKKAHL MEIQVNGGTV AEKLDWARER
210 220 230 240 250
LEQQVPVNQV FGQDEMIDVI GVTKGKGYKG VTSRWHTKKL PRKTHRGLRK
260 270 280 290 300
VACIGAWHPA RVAFSVARAG QKGYHHRTEI NKKIYKIGQG YLIKDGKLIK
310 320 330 340 350
NNASTDYDLS DKSINPLGGF VHYGEVTNDF IMLKGCVVGT KKRVLTLRKS
360 370 380 390 400
LLVQTKRRAL EKIDLKFIDT TSKFGHGRFQ TMEEKKAFMG PLKKDRIAKE
EGA
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WNS0 | A0A087WNS0_MOUSE | 60S ribosomal protein L3 | Rpl3 | 180 | Annotation score: | ||
A0A087WQK0 | A0A087WQK0_MOUSE | 60S ribosomal protein L3 | Rpl3 | 188 | Annotation score: | ||
A0A2R8VHN4 | A0A2R8VHN4_MOUSE | 60S ribosomal protein L3 | Rpl3 | 134 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 265 | S → T in CAA68370 (PubMed:2628163).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00225 mRNA Translation: CAA68370.1 U89417 mRNA Translation: AAC36524.1 AK088993 mRNA Translation: BAC40691.1 AK144518 mRNA Translation: BAE25922.1 AK144816 mRNA Translation: BAE26078.1 AK146502 mRNA Translation: BAE27217.1 AK149494 mRNA Translation: BAE28917.1 AK151596 mRNA Translation: BAE30536.1 AK166433 mRNA Translation: BAE38773.1 AK166825 mRNA Translation: BAE39049.1 AK167787 mRNA Translation: BAE39818.1 AK167947 mRNA Translation: BAE39950.1 AK168040 mRNA Translation: BAE40022.1 AK168059 mRNA Translation: BAE40037.1 AK168126 mRNA Translation: BAE40095.1 AK168128 mRNA Translation: BAE40097.1 AK168224 mRNA Translation: BAE40178.1 AK168744 mRNA Translation: BAE40585.1 AK169060 mRNA Translation: BAE40848.1 AK169235 mRNA Translation: BAE41003.1 AK169321 mRNA Translation: BAE41075.1 AK170819 mRNA Translation: BAE42051.1 CH466550 Genomic DNA Translation: EDL04616.1 BC009655 mRNA Translation: AAH09655.1 BC083134 mRNA Translation: AAH83134.1 BC094059 mRNA Translation: AAH94059.1 BC158039 mRNA Translation: AAI58040.1 |
CCDSi | CCDS37144.1 |
RefSeqi | NP_038790.2, NM_013762.2 |
Genome annotation databases
Ensembli | ENSMUST00000081650; ENSMUSP00000080354; ENSMUSG00000060036 |
GeneIDi | 27367 |
KEGGi | mmu:27367 |
UCSCi | uc007wvb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00225 mRNA Translation: CAA68370.1 U89417 mRNA Translation: AAC36524.1 AK088993 mRNA Translation: BAC40691.1 AK144518 mRNA Translation: BAE25922.1 AK144816 mRNA Translation: BAE26078.1 AK146502 mRNA Translation: BAE27217.1 AK149494 mRNA Translation: BAE28917.1 AK151596 mRNA Translation: BAE30536.1 AK166433 mRNA Translation: BAE38773.1 AK166825 mRNA Translation: BAE39049.1 AK167787 mRNA Translation: BAE39818.1 AK167947 mRNA Translation: BAE39950.1 AK168040 mRNA Translation: BAE40022.1 AK168059 mRNA Translation: BAE40037.1 AK168126 mRNA Translation: BAE40095.1 AK168128 mRNA Translation: BAE40097.1 AK168224 mRNA Translation: BAE40178.1 AK168744 mRNA Translation: BAE40585.1 AK169060 mRNA Translation: BAE40848.1 AK169235 mRNA Translation: BAE41003.1 AK169321 mRNA Translation: BAE41075.1 AK170819 mRNA Translation: BAE42051.1 CH466550 Genomic DNA Translation: EDL04616.1 BC009655 mRNA Translation: AAH09655.1 BC083134 mRNA Translation: AAH83134.1 BC094059 mRNA Translation: AAH94059.1 BC158039 mRNA Translation: AAI58040.1 |
CCDSi | CCDS37144.1 |
RefSeqi | NP_038790.2, NM_013762.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6SWA | electron microscopy | 3.10 | B | 1-403 | [»] | |
SMRi | P27659 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 205174, 85 interactors |
ComplexPortali | CPX-5262, 60S cytosolic large ribosomal subunit |
CORUMi | P27659 |
IntActi | P27659, 7 interactors |
MINTi | P27659 |
STRINGi | 10090.ENSMUSP00000080354 |
PTM databases
iPTMneti | P27659 |
PhosphoSitePlusi | P27659 |
SwissPalmi | P27659 |
Proteomic databases
EPDi | P27659 |
jPOSTi | P27659 |
MaxQBi | P27659 |
PaxDbi | P27659 |
PeptideAtlasi | P27659 |
PRIDEi | P27659 |
ProteomicsDBi | 299902 |
Protocols and materials databases
Antibodypediai | 1245, 277 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000081650; ENSMUSP00000080354; ENSMUSG00000060036 |
GeneIDi | 27367 |
KEGGi | mmu:27367 |
UCSCi | uc007wvb.2, mouse |
Organism-specific databases
CTDi | 6122 |
MGIi | MGI:1351605, Rpl3 |
Phylogenomic databases
eggNOGi | KOG0746, Eukaryota |
GeneTreei | ENSGT00390000017606 |
HOGENOMi | CLU_033361_2_1_1 |
InParanoidi | P27659 |
OMAi | HQRTEYN |
OrthoDBi | 793191at2759 |
TreeFami | TF300555 |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 27367, 19 hits in 32 CRISPR screens |
ChiTaRSi | Rpl3, mouse |
PROi | PR:P27659 |
RNActi | P27659, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000060036, Expressed in ventricular zone and 89 other tissues |
ExpressionAtlasi | P27659, baseline and differential |
Genevisiblei | P27659, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000597, Ribosomal_L3 IPR019926, Ribosomal_L3_CS IPR009000, Transl_B-barrel_sf |
Pfami | View protein in Pfam PF00297, Ribosomal_L3, 1 hit |
SUPFAMi | SSF50447, SSF50447, 1 hit |
PROSITEi | View protein in PROSITE PS00474, RIBOSOMAL_L3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RL3_MOUSE | |
Accessioni | P27659Primary (citable) accession number: P27659 Secondary accession number(s): O89071, Q91VJ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Ribosomal proteins
Ribosomal proteins families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families