UniProtKB - P27619 (DYN_DROME)
Protein
Dynamin
Gene
shi
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting.
Miscellaneous
Shibire mutation is the cause of temperature-sensitive paralysis. This is believed to be due to a reversible block of endocytosis, which prevents membrane cycling and thus depletes synaptic vesicles.
'Shibire' means 'paralyzed' in Japanese.
Catalytic activityi
- EC:3.6.5.5
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 33 – 41 | GTPBy similarity | 9 | |
Nucleotide bindingi | 200 – 206 | GTPBy similarity | 7 | |
Nucleotide bindingi | 231 – 234 | GTPBy similarity | 4 |
GO - Molecular functioni
- actin binding Source: FlyBase
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- microtubule binding Source: FlyBase
GO - Biological processi
- adherens junction maintenance Source: FlyBase
- border follicle cell migration Source: FlyBase
- bulk synaptic vesicle endocytosis Source: FlyBase
- cellularization Source: FlyBase
- chemical synaptic transmission Source: FlyBase
- clathrin-dependent endocytosis Source: FlyBase
- clathrin-dependent endocytosis involved in vitellogenesis Source: FlyBase
- clathrin-dependent synaptic vesicle endocytosis Source: FlyBase
- compound eye retinal cell programmed cell death Source: FlyBase
- conditioned taste aversion Source: FlyBase
- cortical actin cytoskeleton organization Source: FlyBase
- dorsal appendage formation Source: FlyBase
- dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
- endocytosis Source: FlyBase
- epithelial cell migration, open tracheal system Source: FlyBase
- extracellular matrix organization Source: FlyBase
- larval feeding behavior Source: FlyBase
- learning Source: FlyBase
- membrane fission Source: FlyBase
- membrane fusion Source: GO_Central
- memory Source: FlyBase
- mitochondrial fission Source: GO_Central
- mitotic actomyosin contractile ring assembly Source: FlyBase
- morphogenesis of a polarized epithelium Source: FlyBase
- negative regulation of protein phosphorylation Source: UniProtKB
- olfactory learning Source: FlyBase
- open tracheal system development Source: FlyBase
- ovarian follicle cell development Source: FlyBase
- positive regulation of actin filament polymerization Source: UniProtKB
- positive regulation of autophagy Source: FlyBase
- postsynaptic neurotransmitter receptor internalization Source: GO_Central
- proboscis extension reflex Source: FlyBase
- protein localization to plasma membrane Source: FlyBase
- receptor internalization Source: GO_Central
- regulation of actin cytoskeleton organization Source: FlyBase
- regulation of actin filament-based process Source: FlyBase
- regulation of growth Source: FlyBase
- regulation of short-term neuronal synaptic plasticity Source: FlyBase
- regulation of synapse structure or activity Source: FlyBase
- regulation of tube architecture, open tracheal system Source: FlyBase
- salivary gland morphogenesis Source: FlyBase
- short-term memory Source: FlyBase
- sperm individualization Source: FlyBase
- synaptic vesicle budding from presynaptic endocytic zone membrane Source: FlyBase
- synaptic vesicle endocytosis Source: FlyBase
- synaptic vesicle membrane organization Source: FlyBase
- synaptic vesicle recycling Source: FlyBase
- synaptic vesicle transport Source: FlyBase
- syncytial blastoderm mitotic cell cycle Source: FlyBase
- viral entry into host cell Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Motor protein |
Biological process | Endocytosis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 1994 |
Reactomei | R-DME-1169408, ISG15 antiviral mechanism R-DME-190873, Gap junction degradation R-DME-196025, Formation of annular gap junctions R-DME-432720, Lysosome Vesicle Biogenesis R-DME-432722, Golgi Associated Vesicle Biogenesis R-DME-437239, Recycling pathway of L1 R-DME-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:shi ORF Names:CG18102 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003392, shi |
Subcellular locationi
Cytoskeleton
Cytoplasm and Cytosol
Note: Microtubule-associated.
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: GO_Central
- mitotic spindle Source: FlyBase
Mitochondrion
- mitochondrial membrane Source: GO_Central
Plasma Membrane
- plasma membrane Source: FlyBase
- postsynaptic endocytic zone membrane Source: GO_Central
Other locations
- axon Source: GO_Central
- cytoplasm Source: FlyBase
- cytoplasmic vesicle Source: GO_Central
- dendritic spine head Source: GO_Central
- membrane Source: GO_Central
- postsynaptic density Source: GO_Central
- presynapse Source: GOC
- sperm individualization complex Source: FlyBase
- synapse Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 141 | G → S in allele shi-TS2; temperature sensitive larval and adult paralysis. 1 Publication | 1 | |
Mutagenesisi | 268 | G → D in allele shi-TS1; temperature sensitive larval and adult paralysis. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206576 | 1 – 877 | DynaminAdd BLAST | 877 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 756 | Phosphoserine1 Publication | 1 | |
Modified residuei | 764 | Phosphoserine1 Publication | 1 | |
Modified residuei | 767 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P27619 |
PRIDEi | P27619 |
PTM databases
iPTMneti | P27619 |
Expressioni
Gene expression databases
Bgeei | FBgn0003392, Expressed in head and 45 other tissues |
ExpressionAtlasi | P27619, baseline and differential |
Genevisiblei | P27619, DM |
Interactioni
GO - Molecular functioni
- actin binding Source: FlyBase
- microtubule binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 69991, 58 interactors |
DIPi | DIP-17621N |
IntActi | P27619, 4 interactors |
STRINGi | 7227.FBpp0305866 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 289 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 267 | |
Domaini | 513 – 621 | PHPROSITE-ProRule annotationAdd BLAST | 109 | |
Domaini | 650 – 741 | GEDPROSITE-ProRule annotationAdd BLAST | 92 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 40 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 59 – 61 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 131 – 134 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 200 – 203 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 230 – 233 | G5 motifPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 744 – 827 | Pro-richAdd BLAST | 84 |
Sequence similaritiesi
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000166903 |
InParanoidi | P27619 |
PhylomeDBi | P27619 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027188, DNM2 IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF23, PTHR11566:SF23, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P27619-1) [UniParc]FASTAAdd to basket
Also known as: 4, D, F
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDSLITIVNK LQDAFTSLGV HMQLDLPQIA VVGGQSAGKS SVLENFVGKD
60 70 80 90 100
FLPRGSGIVT RRPLILQLIN GVTEYGEFLH IKGKKFSSFD EIRKEIEDET
110 120 130 140 150
DRVTGSNKGI SNIPINLRVY SPHVLNLTLI DLPGLTKVAI GDQPVDIEQQ
160 170 180 190 200
IKQMIFQFIR KETCLILAVT PANTDLANSD ALKLAKEVDP QGVRTIGVIT
210 220 230 240 250
KLDLMDEGTD ARDILENKLL PLRRGYIGVV NRSQKDIEGR KDIHQALAAE
260 270 280 290 300
RKFFLSHPSY RHMADRLGTP YLQRVLNQQL TNHIRDTLPG LRDKLQKQML
310 320 330 340 350
TLEKEVEEFK HFQPGDASIK TKAMLQMIQQ LQSDFERTIE GSGSALVNTN
360 370 380 390 400
ELSGGAKINR IFHERLRFEI VKMACDEKEL RREISFAIRN IHGIRVGLFT
410 420 430 440 450
PDMAFEAIVK RQIALLKEPV IKCVDLVVQE LSVVVRMCTA KMSRYPRLRE
460 470 480 490 500
ETERIITTHV RQREHSCKEQ ILLLIDFELA YMNTNHEDFI GFANAQNKSE
510 520 530 540 550
NANKTGTRQL GNQVIRKGHM VIQNLGIMKG GSRPYWFVLT SESISWYKDE
560 570 580 590 600
DEKEKKFMLP LDGLKLRDIE QGFMSMSRRV TFALFSPDGR NVYKDYKQLE
610 620 630 640 650
LSCETVEDVE SWKASFLRAG VYPEKQETQE NGDESASEES SSDPQLERQV
660 670 680 690 700
ETIRNLVDSY MKIVTKTTRD MVPKAIMMLI INNAKDFING ELLAHLYASG
710 720 730 740 750
DQAQMMEESA ESATRREEML RMYRACKDAL QIIGDVSMAT VSSPLPPPVK
760 770 780 790 800
NDWLPSGLDN PRLSPPSPGG VRGKPGPPAQ SSLGGRNPPL PPSTGRPAPA
810 820 830 840 850
IPNRPGGGAP PLPGGRPGGS LPPPMLPSRV SGAVGGAIVQ QSGANRYVPE
860 870
SMRGQVNQAV GQAAINELSN AFSSRFK
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JJA4 | E1JJA4_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 883 | Annotation score: | ||
E1JJA5 | E1JJA5_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 836 | Annotation score: | ||
M9PJN9 | M9PJN9_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 834 | Annotation score: | ||
X2JE15 | X2JE15_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 881 | Annotation score: | ||
A4V4I8 | A4V4I8_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 877 | Annotation score: | ||
A4V4J0 | A4V4J0_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 830 | Annotation score: | ||
M9PHQ0 | M9PHQ0_DROME | Dynamin GTPase | shi anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, Ddyn, dDyn | 896 | Annotation score: |
Sequence cautioni
The sequence CAA42061 differs from that shown. Chimeric cDNA. There is a 6 amino acid insertion at position 634 due to a chimera of DNA from chromosome arms X and 3L.Curated
The sequence CAA42067 differs from that shown. Chimeric cDNA. There is a 6 amino acid insertion at position 634 due to a chimera of DNA from chromosome arms X and 3L.Curated
The sequence CAA42068 differs from that shown. Chimeric cDNA. There is a 6 amino acid insertion at position 634 due to a chimera of DNA from chromosome arms X and 3L.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 594 | K → R in CAA42067 (PubMed:1828536).Curated | 1 | |
Sequence conflicti | 594 | K → R in CAA42068 (PubMed:1828536).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001330 | 830 | V → R in isoform 2. 2 Publications | 1 | |
Alternative sequenceiVSP_001331 | 831 – 877 | Missing in isoform 2. 2 PublicationsAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59448 mRNA Translation: CAA42067.1 Sequence problems. X59449 mRNA Translation: CAA42068.1 Sequence problems. X59435 mRNA Translation: CAA42061.1 Sequence problems. X59436 mRNA Translation: CAA42062.1 AE014298 Genomic DNA Translation: AAF48536.2 AE014298 Genomic DNA Translation: AAS65368.1 BT010049 mRNA Translation: AAQ22518.1 |
PIRi | S15413 S16130 S17974 S17975 S34399 |
RefSeqi | NP_001036278.1, NM_001042813.2 [P27619-2] NP_001036279.1, NM_001042814.2 [P27619-2] NP_001162766.1, NM_001169295.1 [P27619-1] NP_001162768.1, NM_001169297.2 NP_524853.2, NM_080114.4 [P27619-2] NP_727910.1, NM_167470.3 [P27619-1] NP_727911.1, NM_167471.5 [P27619-2] NP_996465.1, NM_206742.1 [P27619-1] NP_996466.1, NM_206743.2 [P27619-1] NP_996467.1, NM_206744.3 [P27619-2] NP_996468.1, NM_206745.3 [P27619-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0074118; FBpp0073928; FBgn0003392 [P27619-2] FBtr0074119; FBpp0073929; FBgn0003392 [P27619-2] FBtr0074121; FBpp0089278; FBgn0003392 [P27619-2] FBtr0074122; FBpp0089279; FBgn0003392 [P27619-2] FBtr0074123; FBpp0089280; FBgn0003392 [P27619-1] FBtr0074124; FBpp0089277; FBgn0003392 [P27619-1] FBtr0111036; FBpp0110335; FBgn0003392 [P27619-2] FBtr0111037; FBpp0110336; FBgn0003392 [P27619-2] FBtr0301594; FBpp0290809; FBgn0003392 [P27619-1] FBtr0301595; FBpp0290810; FBgn0003392 [P27619-1] |
GeneIDi | 45928 |
KEGGi | dme:Dmel_CG18102 |
UCSCi | CG18102-RA, d. melanogaster [P27619-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59448 mRNA Translation: CAA42067.1 Sequence problems. X59449 mRNA Translation: CAA42068.1 Sequence problems. X59435 mRNA Translation: CAA42061.1 Sequence problems. X59436 mRNA Translation: CAA42062.1 AE014298 Genomic DNA Translation: AAF48536.2 AE014298 Genomic DNA Translation: AAS65368.1 BT010049 mRNA Translation: AAQ22518.1 |
PIRi | S15413 S16130 S17974 S17975 S34399 |
RefSeqi | NP_001036278.1, NM_001042813.2 [P27619-2] NP_001036279.1, NM_001042814.2 [P27619-2] NP_001162766.1, NM_001169295.1 [P27619-1] NP_001162768.1, NM_001169297.2 NP_524853.2, NM_080114.4 [P27619-2] NP_727910.1, NM_167470.3 [P27619-1] NP_727911.1, NM_167471.5 [P27619-2] NP_996465.1, NM_206742.1 [P27619-1] NP_996466.1, NM_206743.2 [P27619-1] NP_996467.1, NM_206744.3 [P27619-2] NP_996468.1, NM_206745.3 [P27619-2] |
3D structure databases
SMRi | P27619 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 69991, 58 interactors |
DIPi | DIP-17621N |
IntActi | P27619, 4 interactors |
STRINGi | 7227.FBpp0305866 |
PTM databases
iPTMneti | P27619 |
Proteomic databases
PaxDbi | P27619 |
PRIDEi | P27619 |
Genome annotation databases
EnsemblMetazoai | FBtr0074118; FBpp0073928; FBgn0003392 [P27619-2] FBtr0074119; FBpp0073929; FBgn0003392 [P27619-2] FBtr0074121; FBpp0089278; FBgn0003392 [P27619-2] FBtr0074122; FBpp0089279; FBgn0003392 [P27619-2] FBtr0074123; FBpp0089280; FBgn0003392 [P27619-1] FBtr0074124; FBpp0089277; FBgn0003392 [P27619-1] FBtr0111036; FBpp0110335; FBgn0003392 [P27619-2] FBtr0111037; FBpp0110336; FBgn0003392 [P27619-2] FBtr0301594; FBpp0290809; FBgn0003392 [P27619-1] FBtr0301595; FBpp0290810; FBgn0003392 [P27619-1] |
GeneIDi | 45928 |
KEGGi | dme:Dmel_CG18102 |
UCSCi | CG18102-RA, d. melanogaster [P27619-1] |
Organism-specific databases
CTDi | 45928 |
FlyBasei | FBgn0003392, shi |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000166903 |
InParanoidi | P27619 |
PhylomeDBi | P27619 |
Enzyme and pathway databases
BRENDAi | 3.6.5.5, 1994 |
Reactomei | R-DME-1169408, ISG15 antiviral mechanism R-DME-190873, Gap junction degradation R-DME-196025, Formation of annular gap junctions R-DME-432720, Lysosome Vesicle Biogenesis R-DME-432722, Golgi Associated Vesicle Biogenesis R-DME-437239, Recycling pathway of L1 R-DME-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 45928, 3 hits in 3 CRISPR screens |
ChiTaRSi | shi, fly |
GenomeRNAii | 45928 |
PROi | PR:P27619 |
Gene expression databases
Bgeei | FBgn0003392, Expressed in head and 45 other tissues |
ExpressionAtlasi | P27619, baseline and differential |
Genevisiblei | P27619, DM |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR027188, DNM2 IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR000375, Dynamin_stalk IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase IPR011993, PH-like_dom_sf IPR001849, PH_domain |
PANTHERi | PTHR11566, PTHR11566, 1 hit PTHR11566:SF23, PTHR11566:SF23, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit PF00169, PH, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit SM00233, PH, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DYN_DROME | |
Accessioni | P27619Primary (citable) accession number: P27619 Secondary accession number(s): Q0KHS4, Q9VXM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | February 28, 2003 | |
Last modified: | April 7, 2021 | |
This is version 212 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families