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UniProtKB - P27614 (CBPS_YEAST)
Protein
Carboxypeptidase S
Gene
CPS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Necessary for use of certain peptides as sole nitrogen source. May also cleave intracellularly generated peptides to recycle amino acids for protein synthesis.
Miscellaneous
Present with 721 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- Release of a C-terminal amino acid from a peptide in which glycine is the penultimate amino acid, e.g. Z-Gly-|-Leu. EC:3.4.17.4
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 168 | Zinc 2By similarity | 1 | |
Active sitei | 170 | By similarity | 1 | |
Metal bindingi | 205 | Zinc 1By similarity | 1 | |
Metal bindingi | 205 | Zinc 2By similarity | 1 | |
Active sitei | 239 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 240 | Zinc 1By similarity | 1 | |
Metal bindingi | 268 | Zinc 2By similarity | 1 | |
Metal bindingi | 547 | Zinc 1By similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: SGD
- metal ion binding Source: UniProtKB-KW
- metallocarboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- nitrogen compound metabolic process Source: SGD
- proteolysis involved in cellular protein catabolic process Source: SGD
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SCE-9673163, Oleoyl-phe metabolism |
Protein family/group databases
MEROPSi | M20.002 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CPS1 Synonyms:CPS Ordered Locus Names:YJL172W ORF Names:J0510 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003708, CPS1 |
VEuPathDBi | FungiDB:YJL172W |
Subcellular locationi
Vacuole
- Vacuole membrane 2 Publications; Single-pass membrane protein 2 Publications
Note: Lysosome-like vacuoles.
Vacuole
- fungal-type vacuole Source: SGD
- fungal-type vacuole lumen Source: SGD
- vacuolar lumen Source: CAFA
- vacuolar membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 40 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 41 – 576 | LumenalSequence analysisAdd BLAST | 536 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185271 | 1 – 576 | Carboxypeptidase SAdd BLAST | 576 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 8 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Glycosylationi | 88 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 228 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 381 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 525 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Glycosylated.
Ubiquitinated. Ubiquitination mediates sorting into internal vesicles in late endosomes. TUL1 is required for ubiquitination.1 Publication
Keywords - PTMi
Glycoprotein, Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P27614 |
PaxDbi | P27614 |
PRIDEi | P27614 |
PTM databases
iPTMneti | P27614 |
Interactioni
Subunit structurei
yscS is synthesized as one polypeptide chain precursor which after carbohydrate modification in the secretory pathway yields two active precursor molecules. The proteolytically unprocessed forms are associated with the membrane, whereas the mature forms of the enzyme are soluble.
Protein-protein interaction databases
BioGRIDi | 33588, 69 interactors |
DIPi | DIP-5566N |
IntActi | P27614, 4 interactors |
MINTi | P27614 |
STRINGi | 4932.YJL172W |
Miscellaneous databases
RNActi | P27614, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 44 – 65 | DisorderedSequence analysisAdd BLAST | 22 |
Domaini
The transmembrane domain contains polar residues that mediate the recognition by TUL1.
Sequence similaritiesi
Belongs to the peptidase M20A family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2275, Eukaryota |
HOGENOMi | CLU_021802_11_0_1 |
InParanoidi | P27614 |
OMAi | NYRVALH |
Family and domain databases
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR036264, Bact_exopeptidase_dim_dom IPR017141, Pept_M20_carboxypep IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF037217, Carboxypeptidase_S, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
PROSITEi | View protein in PROSITE PS00758, ARGE_DAPE_CPG2_1, 1 hit PS00759, ARGE_DAPE_CPG2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P27614-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIALPVEKAP RKSLWQRHRA FISGIVALII IGTFFLTSGL HPAPPHEAKR
60 70 80 90 100
PHHGKGPMHS PKCEKIEPLS PSFKHSVDTI LHDPAFRNSS IEKLSNAVRI
110 120 130 140 150
PTVVQDKNPN PADDPDFYKH FYELHDYFEK TFPNIHKHLK LEKVNELGLL
160 170 180 190 200
YTWEGSDPDL KPLLLMAHQD VVPVNNETLS SWKFPPFSGH YDPETDFVWG
210 220 230 240 250
RGSNDCKNLL IAEFEAIEQL LIDGFKPNRT IVMSLGFDEE ASGTLGAASL
260 270 280 290 300
ASFLHERYGD DGIYSIIDEG EGIMEVDKDV FVATPINAEK GYVDFEVSIL
310 320 330 340 350
GHGGHSSVPP DHTTIGIASE LITEFEANPF DYEFEFDNPI YGLLTCAAEH
360 370 380 390 400
SKSLSKDVKK TILGAPFCPR RKDKLVEYIS NQSHLRSLIR TTQAVDIING
410 420 430 440 450
GVKANALPET TRFLINHRIN LHSSVAEVFE RNIEYAKKIA EKYGYGLSKN
460 470 480 490 500
GDDYIIPETE LGHIDITLLR ELEPAPLSPS SGPVWDILAG TIQDVFENGV
510 520 530 540 550
LQNNEEFYVT TGLFSGNTDT KYYWNLSKNI YRFVGSIIDI DLLKTLHSVN
560 570
EHVDVPGHLS AIAFVYEYIV NVNEYA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 387 | S → T in CAA40571 (PubMed:1709881).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57316 Genomic DNA Translation: CAA40571.1 X63068 Genomic DNA Translation: CAA44790.1 Z49447 Genomic DNA Translation: CAA89467.1 BK006943 Genomic DNA Translation: DAA08632.1 |
PIRi | S16693 |
RefSeqi | NP_012363.1, NM_001181605.1 |
Genome annotation databases
EnsemblFungii | YJL172W_mRNA; YJL172W; YJL172W |
GeneIDi | 853267 |
KEGGi | sce:YJL172W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57316 Genomic DNA Translation: CAA40571.1 X63068 Genomic DNA Translation: CAA44790.1 Z49447 Genomic DNA Translation: CAA89467.1 BK006943 Genomic DNA Translation: DAA08632.1 |
PIRi | S16693 |
RefSeqi | NP_012363.1, NM_001181605.1 |
3D structure databases
SMRi | P27614 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33588, 69 interactors |
DIPi | DIP-5566N |
IntActi | P27614, 4 interactors |
MINTi | P27614 |
STRINGi | 4932.YJL172W |
Protein family/group databases
MEROPSi | M20.002 |
PTM databases
iPTMneti | P27614 |
Proteomic databases
MaxQBi | P27614 |
PaxDbi | P27614 |
PRIDEi | P27614 |
Protocols and materials databases
DNASUi | 853267 |
Genome annotation databases
EnsemblFungii | YJL172W_mRNA; YJL172W; YJL172W |
GeneIDi | 853267 |
KEGGi | sce:YJL172W |
Organism-specific databases
SGDi | S000003708, CPS1 |
VEuPathDBi | FungiDB:YJL172W |
Phylogenomic databases
eggNOGi | KOG2275, Eukaryota |
HOGENOMi | CLU_021802_11_0_1 |
InParanoidi | P27614 |
OMAi | NYRVALH |
Enzyme and pathway databases
Reactomei | R-SCE-9673163, Oleoyl-phe metabolism |
Miscellaneous databases
PROi | PR:P27614 |
RNActi | P27614, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR036264, Bact_exopeptidase_dim_dom IPR017141, Pept_M20_carboxypep IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF037217, Carboxypeptidase_S, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
PROSITEi | View protein in PROSITE PS00758, ARGE_DAPE_CPG2_1, 1 hit PS00759, ARGE_DAPE_CPG2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CBPS_YEAST | |
Accessioni | P27614Primary (citable) accession number: P27614 Secondary accession number(s): D6VW16 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families