UniProtKB - P27605 (HPRT_RAT)
Protein
Hypoxanthine-guanine phosphoribosyltransferase
Gene
Hprt1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity
Catalytic activityi
Cofactori
Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity
: IMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.Proteins known to be involved in this subpathway in this organism are:
- Hypoxanthine-guanine phosphoribosyltransferase (Hprt1), Hypoxanthine phosphoribosyltransferase (Prtfdc1), Hypoxanthine phosphoribosyltransferase
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | GMPBy similarity | 1 | |
Active sitei | 138 | Proton acceptorBy similarity | 1 | |
Binding sitei | 166 | GMPBy similarity | 1 | |
Metal bindingi | 194 | MagnesiumBy similarity | 1 | |
Binding sitei | 194 | GMP; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 134 – 142 | GMPBy similarity | 9 | |
Nucleotide bindingi | 186 – 188 | GMPBy similarity | 3 |
GO - Molecular functioni
- guanine phosphoribosyltransferase activity Source: UniProtKB
- hypoxanthine phosphoribosyltransferase activity Source: RGD
- magnesium ion binding Source: GO_Central
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- adenine salvage Source: GO_Central
- brain development Source: RGD
- cellular response to insulin stimulus Source: RGD
- GMP catabolic process Source: UniProtKB
- GMP salvage Source: GO_Central
- guanine salvage Source: UniProtKB
- hypoxanthine metabolic process Source: RGD
- hypoxanthine salvage Source: UniProtKB
- IMP metabolic process Source: UniProtKB
- IMP salvage Source: GO_Central
- purine ribonucleoside salvage Source: GO_Central
- spermatogenesis Source: RGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Ligand | Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-74217 Purine salvage |
SABIO-RKi | P27605 |
UniPathwayi | UPA00591;UER00648 |
Names & Taxonomyi
Protein namesi | Recommended name: Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)Short name: HGPRT Short name: HGPRTase |
Gene namesi | Name:Hprt1 Synonyms:Hprt |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2826 Hprt1 |
Subcellular locationi
Other locations
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139591 | 1 – 218 | Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST | 218 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 103 | N6-acetyllysineBy similarity | 1 | |
Cross-linki | 115 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 115 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 142 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P27605 |
PaxDbi | P27605 |
PRIDEi | P27605 |
2D gel databases
World-2DPAGEi | 0004:P27605 |
PTM databases
iPTMneti | P27605 |
PhosphoSitePlusi | P27605 |
SwissPalmi | P27605 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000031367 Expressed in 9 organ(s), highest expression level in heart |
Genevisiblei | P27605 RN |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
BioGridi | 246627, 1 interactor |
IntActi | P27605, 2 interactors |
STRINGi | 10116.ENSRNOP00000043388 |
Family & Domainsi
Sequence similaritiesi
Belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG3367 Eukaryota COG0634 LUCA |
GeneTreei | ENSGT00940000155028 |
HOGENOMi | HOG000236521 |
InParanoidi | P27605 |
KOi | K00760 |
OMAi | TMDWMAV |
OrthoDBi | 1537610at2759 |
PhylomeDBi | P27605 |
TreeFami | TF313367 |
Family and domain databases
CDDi | cd06223 PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
InterProi | View protein in InterPro IPR005904 Hxn_phspho_trans IPR000836 PRibTrfase_dom IPR029057 PRTase-like |
Pfami | View protein in Pfam PF00156 Pribosyltran, 1 hit |
SUPFAMi | SSF53271 SSF53271, 1 hit |
TIGRFAMsi | TIGR01203 HGPRTase, 1 hit |
PROSITEi | View protein in PROSITE PS00103 PUR_PYR_PR_TRANSFER, 1 hit |
i Sequence
Sequence statusi: Complete.
P27605-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTLSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEHFR
210
DLNHVCVISE SGKAKYKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63983 mRNA Translation: AAA41350.1 S79292 mRNA Translation: AAB21288.1 X62085 mRNA Translation: CAA43997.1 AF001282 AF001281 Genomic DNA Translation: AAB65640.1 BC098629 mRNA Translation: AAH98629.1 U06049 Genomic DNA Translation: AAA56887.1 |
PIRi | S18140 |
RefSeqi | NP_036715.1, NM_012583.2 XP_008771881.1, XM_008773659.2 |
Genome annotation databases
Ensembli | ENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367 ENSRNOT00000065935; ENSRNOP00000062740; ENSRNOG00000048561 |
GeneIDi | 103689983 24465 |
KEGGi | rno:103689983 rno:24465 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63983 mRNA Translation: AAA41350.1 S79292 mRNA Translation: AAB21288.1 X62085 mRNA Translation: CAA43997.1 AF001282 AF001281 Genomic DNA Translation: AAB65640.1 BC098629 mRNA Translation: AAH98629.1 U06049 Genomic DNA Translation: AAA56887.1 |
PIRi | S18140 |
RefSeqi | NP_036715.1, NM_012583.2 XP_008771881.1, XM_008773659.2 |
3D structure databases
SMRi | P27605 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 246627, 1 interactor |
IntActi | P27605, 2 interactors |
STRINGi | 10116.ENSRNOP00000043388 |
PTM databases
iPTMneti | P27605 |
PhosphoSitePlusi | P27605 |
SwissPalmi | P27605 |
2D gel databases
World-2DPAGEi | 0004:P27605 |
Proteomic databases
jPOSTi | P27605 |
PaxDbi | P27605 |
PRIDEi | P27605 |
Genome annotation databases
Ensembli | ENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367 ENSRNOT00000065935; ENSRNOP00000062740; ENSRNOG00000048561 |
GeneIDi | 103689983 24465 |
KEGGi | rno:103689983 rno:24465 |
Organism-specific databases
CTDi | 3251 |
RGDi | 2826 Hprt1 |
Phylogenomic databases
eggNOGi | KOG3367 Eukaryota COG0634 LUCA |
GeneTreei | ENSGT00940000155028 |
HOGENOMi | HOG000236521 |
InParanoidi | P27605 |
KOi | K00760 |
OMAi | TMDWMAV |
OrthoDBi | 1537610at2759 |
PhylomeDBi | P27605 |
TreeFami | TF313367 |
Enzyme and pathway databases
UniPathwayi | UPA00591;UER00648 |
Reactomei | R-RNO-74217 Purine salvage |
SABIO-RKi | P27605 |
Miscellaneous databases
PROi | PR:P27605 |
Gene expression databases
Bgeei | ENSRNOG00000031367 Expressed in 9 organ(s), highest expression level in heart |
Genevisiblei | P27605 RN |
Family and domain databases
CDDi | cd06223 PRTases_typeI, 1 hit |
Gene3Di | 3.40.50.2020, 1 hit |
InterProi | View protein in InterPro IPR005904 Hxn_phspho_trans IPR000836 PRibTrfase_dom IPR029057 PRTase-like |
Pfami | View protein in Pfam PF00156 Pribosyltran, 1 hit |
SUPFAMi | SSF53271 SSF53271, 1 hit |
TIGRFAMsi | TIGR01203 HGPRTase, 1 hit |
PROSITEi | View protein in PROSITE PS00103 PUR_PYR_PR_TRANSFER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HPRT_RAT | |
Accessioni | P27605Primary (citable) accession number: P27605 Secondary accession number(s): P70469, Q4KMC5, Q62926 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | November 13, 2019 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways