UniProtKB - P27600 (GNA12_MOUSE)
Guanine nucleotide-binding protein subunit alpha-12
Gna12
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 69 | Magnesium1 Publication | 1 | |
Metal bindingi | 206 | Magnesium1 Publication | 1 | |
Binding sitei | 351 | GTP; via amide nitrogenCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 65 – 70 | GTPCombined sources1 Publication | 6 | |
Nucleotide bindingi | 200 – 203 | GTPCombined sources1 Publication | 4 | |
Nucleotide bindingi | 294 – 297 | GTPCombined sources1 Publication | 4 |
GO - Molecular functioni
- D5 dopamine receptor binding Source: MGI
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: MGI
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell differentiation Source: MGI
- embryonic digit morphogenesis Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- intracellular signal transduction Source: MGI
- in utero embryonic development Source: MGI
- regulation of cell shape Source: MGI
- regulation of fibroblast migration Source: UniProtKB
- regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- regulation of TOR signaling Source: UniProtKB
- response to drug Source: Ensembl
- Rho protein signal transduction Source: MGI
Keywordsi
Molecular function | Transducer |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-416482, G alpha (12/13) signalling events R-MMU-456926, Thrombin signalling through proteinase activated receptors (PARs) |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein subunit alpha-12Short name: G alpha-12 Short name: G-protein subunit alpha-12 |
Gene namesi | Name:Gna12 Synonyms:Gna-12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95767, Gna12 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
- Lateral cell membrane By similarity; Lipid-anchor By similarity
Other locations
- Cytoplasm By similarity
Note: CDH1 enhances cell membrane localization.By similarity
Plasma Membrane
- brush border membrane Source: MGI
- heterotrimeric G-protein complex Source: GO_Central
- lateral plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | S → G: Results in myristoylation of G-2, which restores the transformation activity; when associated with S-6. 1 Publication | 1 | |
Mutagenesisi | 6 | R → S: Results in myristoylation of G-2, which restores the transformation activity; when associated with G-2. 1 Publication | 1 | |
Mutagenesisi | 11 | C → S: Abolishes palmitoylation and transformation activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000203771 | 1 – 379 | Guanine nucleotide-binding protein subunit alpha-12Add BLAST | 379 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 11 | S-palmitoyl cysteine1 Publication | 1 | |
Modified residuei | 206 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | P27600 |
jPOSTi | P27600 |
MaxQBi | P27600 |
PaxDbi | P27600 |
PeptideAtlasi | P27600 |
PRIDEi | P27600 |
PTM databases
iPTMneti | P27600 |
PhosphoSitePlusi | P27600 |
SwissPalmi | P27600 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000000149, Expressed in cardiac ventricle and 291 other tissues |
Genevisiblei | P27600, MM |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma (PubMed:16388592). The alpha chain contains the guanine nucleotide binding site (PubMed:16388592).
Interacts with UBXD5 (By similarity).
Interacts (in GTP-bound form) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane (By similarity).
Interacts with RGS22 (By similarity).
Interacts (via N-terminus) with NAPA; the interaction promotes CDH5 localization to plasma membrane (By similarity).
Interacts with CTNND1 (via N-terminus); the interaction regulates CDH1-mediated cell-cell adhesion (PubMed:15240885).
Interacts with PPP2R1A; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT (By similarity).
Interacts (in GTP-bound form) with ARHGEF1 (PubMed:16388592).
Interacts (in GTP-bound form) with ARHGEF11 (via RGS domain) (By similarity).
Interacts (in GTP-bound form) with ARHGEF12 (via RGS domain) (PubMed:16388592).
By similarity2 PublicationsGO - Molecular functioni
- D5 dopamine receptor binding Source: MGI
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
IntActi | P27600, 3 interactors |
MINTi | P27600 |
STRINGi | 10090.ENSMUSP00000000153 |
Miscellaneous databases
RNActi | P27600, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P27600 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27600 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 54 – 379 | G-alphaPROSITE-ProRule annotationAdd BLAST | 326 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 70 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 198 – 206 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 221 – 230 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 290 – 297 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 349 – 354 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000157636 |
InParanoidi | P27600 |
OrthoDBi | 754573at2759 |
PhylomeDBi | P27600 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR000469, Gprotein_alpha_12/13 IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00440, GPROTEINA12 |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR
60 70 80 90 100
AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL
110 120 130 140 150
KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP
160 170 180 190 200
ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL
210 220 230 240 250
LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV
260 270 280 290 300
SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV
310 320 330 340 350
EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR SKPLFHHFTT
360 370
AIDTENIRFV FHAVKDTILQ ENLKDIMLQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JG40 | A0A0G2JG40_MOUSE | Guanine nucleotide-binding protein ... | Gna12 | 316 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63659 mRNA Translation: AAA37648.1 M57618 mRNA Translation: AAA63302.1 |
CCDSi | CCDS19825.1 |
PIRi | A41095 C33833 |
RefSeqi | NP_034432.1, NM_010302.2 |
Genome annotation databases
Ensembli | ENSMUST00000000153; ENSMUSP00000000153; ENSMUSG00000000149 |
GeneIDi | 14673 |
KEGGi | mmu:14673 |
UCSCi | uc009aih.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63659 mRNA Translation: AAA37648.1 M57618 mRNA Translation: AAA63302.1 |
CCDSi | CCDS19825.1 |
PIRi | A41095 C33833 |
RefSeqi | NP_034432.1, NM_010302.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZCA | X-ray | 2.90 | A/B | 48-379 | [»] | |
SMRi | P27600 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P27600, 3 interactors |
MINTi | P27600 |
STRINGi | 10090.ENSMUSP00000000153 |
PTM databases
iPTMneti | P27600 |
PhosphoSitePlusi | P27600 |
SwissPalmi | P27600 |
Proteomic databases
EPDi | P27600 |
jPOSTi | P27600 |
MaxQBi | P27600 |
PaxDbi | P27600 |
PeptideAtlasi | P27600 |
PRIDEi | P27600 |
Protocols and materials databases
Antibodypediai | 4103, 220 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000000153; ENSMUSP00000000153; ENSMUSG00000000149 |
GeneIDi | 14673 |
KEGGi | mmu:14673 |
UCSCi | uc009aih.1, mouse |
Organism-specific databases
CTDi | 2768 |
MGIi | MGI:95767, Gna12 |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000157636 |
InParanoidi | P27600 |
OrthoDBi | 754573at2759 |
PhylomeDBi | P27600 |
TreeFami | TF300673 |
Enzyme and pathway databases
Reactomei | R-MMU-416482, G alpha (12/13) signalling events R-MMU-456926, Thrombin signalling through proteinase activated receptors (PARs) |
Miscellaneous databases
BioGRID-ORCSi | 14673, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gna12, mouse |
EvolutionaryTracei | P27600 |
PROi | PR:P27600 |
RNActi | P27600, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000000149, Expressed in cardiac ventricle and 291 other tissues |
Genevisiblei | P27600, MM |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR000469, Gprotein_alpha_12/13 IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00440, GPROTEINA12 |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNA12_MOUSE | |
Accessioni | P27600Primary (citable) accession number: P27600 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families