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Protein

T-cell surface glycoprotein CD3 epsilon chain

Gene

CD3E

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-CFA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
CD_antigen: CD3e
Gene namesi
Name:CD3E
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 5

Organism-specific databases

VGNCiVGNC:38955 CD3E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 122ExtracellularSequence analysisAdd BLAST101
Transmembranei123 – 143HelicalSequence analysisAdd BLAST21
Topological domaini144 – 202CytoplasmicSequence analysisAdd BLAST59

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001460522 – 202T-cell surface glycoprotein CD3 epsilon chainAdd BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 96PROSITE-ProRule annotation
Modified residuei183PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei194PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP27597

Expressioni

Gene expression databases

BgeeiENSCAFG00000012802 Expressed in 3 organ(s), highest expression level in liver

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6. Interacts with NCK1. Interacts with NUMB; this interaction is important for TCR-CD3 internalization and subsequent degradation.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000018835

Structurei

3D structure databases

ProteinModelPortaliP27597
SMRiP27597
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 107Ig-likeAdd BLAST71
Domaini173 – 200ITAMPROSITE-ProRule annotationAdd BLAST28

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 187NUMB-binding regionBy similarityAdd BLAST18

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYD1 Eukaryota
ENOG41123JN LUCA
GeneTreeiENSGT00510000048592
HOGENOMiHOG000290664
HOVERGENiHBG102121
InParanoidiP27597
KOiK06451
OMAiVGAWGQE
OrthoDBiEOG091G0PKE
TreeFamiTF335892

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR007110 Ig-like_dom
IPR013783 Ig-like_fold
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SM00077 ITAM, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51055 ITAM_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27597-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQSRNLWRIL GLCLLSVGAW GQDEDFKASD DLTSISPEKR FKVSISGTEV
60 70 80 90 100
VVTCPDVFGY DNIKWEKNDN LVEGASNREL SQKEFSEVDD SGYYACYADS
110 120 130 140 150
IKEKSYLYLR ARVCANCIEV NLMAVVTIIV ADICLTLGLL LMVYYWSKTR
160 170 180 190 200
KANAKPVMRG TGAGSRPRGQ NKEKPPPVPN PDYEPIRKGQ QDLYSGLNQR

GI
Length:202
Mass (Da):22,642
Last modified:August 1, 1992 - v1
Checksum:iE5779E0A44B8316E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55410 mRNA Translation: AAA30834.1
PIRiI46195
RefSeqiNP_001003379.1, NM_001003379.1
UniGeneiCfa.3890

Genome annotation databases

EnsembliENSCAFT00000020302; ENSCAFP00000018835; ENSCAFG00000012802
GeneIDi442981
KEGGicfa:442981

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55410 mRNA Translation: AAA30834.1
PIRiI46195
RefSeqiNP_001003379.1, NM_001003379.1
UniGeneiCfa.3890

3D structure databases

ProteinModelPortaliP27597
SMRiP27597
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000018835

Proteomic databases

PaxDbiP27597

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCAFT00000020302; ENSCAFP00000018835; ENSCAFG00000012802
GeneIDi442981
KEGGicfa:442981

Organism-specific databases

CTDi916
VGNCiVGNC:38955 CD3E

Phylogenomic databases

eggNOGiENOG410IYD1 Eukaryota
ENOG41123JN LUCA
GeneTreeiENSGT00510000048592
HOGENOMiHOG000290664
HOVERGENiHBG102121
InParanoidiP27597
KOiK06451
OMAiVGAWGQE
OrthoDBiEOG091G0PKE
TreeFamiTF335892

Enzyme and pathway databases

ReactomeiR-CFA-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell

Gene expression databases

BgeeiENSCAFG00000012802 Expressed in 3 organ(s), highest expression level in liver

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015484 CD3_esu/gsu/dsu
IPR007110 Ig-like_dom
IPR013783 Ig-like_fold
IPR003598 Ig_sub2
IPR003110 Phos_immunorcpt_sig_ITAM
PANTHERiPTHR10570 PTHR10570, 1 hit
PfamiView protein in Pfam
PF02189 ITAM, 1 hit
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SM00077 ITAM, 1 hit
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit
PS51055 ITAM_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD3E_CANLF
AccessioniPrimary (citable) accession number: P27597
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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