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Protein

Regulatory protein E2

Gene

E2

Organism
Human papillomavirus type 41
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processDNA replication, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein E2UniRule annotation
Gene namesi
Name:E2UniRule annotation
OrganismiHuman papillomavirus type 41
Taxonomic identifieri10589 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeFirstpapillomavirinaeNupapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000006367 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001332211 – 387Regulatory protein E2Add BLAST387

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki310Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)UniRule annotation

Post-translational modificationi

Phosphorylated.UniRule annotation
Sumoylation plays a regulatory role in E2 transcriptional activity.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP27552

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Binds DNA as homodimer. Interacts with protein E1; this interaction greatly increases E1 DNA-binding activity. Interacts with protein L1; this interaction enhances E2-dependent replication and transcription activation. Interacts with protein L2; this interaction inhibits E2 transcriptional activity but not DNA replication function E2. Interacts with protein E7; this interaction inhibits E7 oncogenic activity. Interacts with host TAF1; this interaction modulates E2-dependent transcriptional regulation. Interacts with host BRD4; this interaction mediates E2 transcriptional activation function. Additionally, the interaction with host BRD4 on mitotic chromosomes mediates tethering of the viral genome. Interacts with host TOPBP1; this interaction is required for optimal viral DNA replication.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP27552
SMRiP27552
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 205Transactivation domainUniRule annotationAdd BLAST205
Regioni303 – 387DNA-binding domainUniRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the papillomaviridae E2 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900010D

Family and domain databases

Gene3Di3.30.70.330, 1 hit
HAMAPiMF_04001 PPV_E2, 1 hit
InterProiView protein in InterPro
IPR035975 E2/EBNA1_C_sf
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR000427 Papillomavirus_E2_C
IPR001866 Papillomavirus_E2_N
IPR033668 Reg_prot_E2
IPR036050 Regulatory_protein_E2_N
PfamiView protein in Pfam
PF00511 PPV_E2_C, 1 hit
PF00508 PPV_E2_N, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000672 E2_early_recog_prot_C, 1 hit
SUPFAMiSSF51332 SSF51332, 1 hit
SSF54957 SSF54957, 1 hit

Sequencei

Sequence statusi: Complete.

P27552-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSQMERLLER LDYIQEQILT LYEKDSVDLE DHIRLWNLLR RENAIWYVLR
60 70 80 90 100
QEGHARVGGR AVPAMTVSEA NAKFAIEMQI KLESLKASPY AAEGWSLQET
110 120 130 140 150
TKERYLAEPS RTFKKLGQPV TLMFDNDPEN LTEVVLWKWV YYITPTDEWY
160 170 180 190 200
KARGGIDDTG IYYIDHESVK MYYVRFDMEA ENFSETGTVT YRLGSALVNV
210 220 230 240 250
PEPVTVTDSS STRERTPKVL RPQGSRRRRN EETGEPVAPA PKRRRGAYGR
260 270 280 290 300
RSSPKAQRRT AASPVSRGNG GSSDFTSGES DEGHRVRHRA LRKKTAGVAP
310 320 330 340 350
AEGHYLVGAK GPVNSLRCLR YKWKNKYSGD IMYLGTTFTW TESDGTERCG
360 370 380
SGRFFCAFSN ETKREKFLKS VKIPKNIGLF RAHAEKL
Length:387
Mass (Da):44,230
Last modified:August 1, 1992 - v1
Checksum:i8F8AA2C0395DBE48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56147 Genomic DNA Translation: CAA39615.1
PIRiD43550 W2WL41
RefSeqiNP_040289.1, NC_001354.1

Genome annotation databases

GeneIDi1489278
KEGGivg:1489278

Entry informationi

Entry nameiVE2_HPV41
AccessioniPrimary (citable) accession number: P27552
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 12, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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