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Protein

Replication protein E1

Gene

E1

Organism
Human papillomavirus type 41
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi443 – 450ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication protein E1UniRule annotation (EC:3.6.4.12UniRule annotation)
Alternative name(s):
ATP-dependent helicase E1UniRule annotation
Gene namesi
Name:E1UniRule annotation
OrganismiHuman papillomavirus type 41
Taxonomic identifieri10589 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeFirstpapillomavirinaeNupapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000006367 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001331391 – 614Replication protein E1Add BLAST614

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei94Phosphoserine; by hostUniRule annotation1
Modified residuei104Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

Phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP27551

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Can form hexamers. Interacts with E2 protein; this interaction increases E1 DNA binding specificity. Interacts with host DNA polymerase subunit POLA2. Interacts with host single stranded DNA-binding protein RPA1. Interacts with host TOP1; this interaction stimulates the enzymatic activity of TOP1.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP27551
SMRiP27551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini403 – 567SF3 helicaseUniRule annotationAdd BLAST165

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 316DNA-binding regionUniRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 90Nuclear localization signalUniRule annotation3
Motifi103 – 112Nuclear export signalUniRule annotation10

Sequence similaritiesi

Belongs to the papillomaviridae E1 protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000HF

Family and domain databases

HAMAPiMF_04000 PPV_E1, 1 hit
InterProiView protein in InterPro
IPR001177 DNA_helicase_E1_C_Papillomavir
IPR014000 DNA_helicase_E1_N_Papillomavir
IPR014015 Helicase_SF3_DNA-vir
IPR027417 P-loop_NTPase
IPR016393 Rep_E1_papillomaV
PfamiView protein in Pfam
PF00519 PPV_E1_C, 1 hit
PF00524 PPV_E1_N, 1 hit
PIRSFiPIRSF003383 Rep_E1_papillomaV, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51206 SF3_HELICASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P27551-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASRVSDTGN GNENKENEGT VASDHSEARC SYILFEAECS DGGDDEESME
60 70 80 90 100
DSLVEDLVDD ASVHQGNSLS LFHAQTVEEY EGEIQSLKRK FILSPLHRDV
110 120 130 140 150
AELSPRLAGV SLEENRGKKA RKSLFHDDSG IDSSAVEVSQ LSSTPSAPGP
160 170 180 190 200
DIRLPKPSDI DLEPLFQSRQ RCTHMYSKFK AVYGVSFTDI TRPFKSDKTT
210 220 230 240 250
SQHWVVAAYY LAFDSEISAM EVLLRQQCQF LYIDNNDGII LFFLEYNVQK
260 270 280 290 300
SRTTVYNWFT ANFHYNENRM LANPPRTRNM PAALFFYHRF MGTGGIKHGA
310 320 330 340 350
MPEIIVNQCV VSNQQTDTFE LSRMVQWALD NDLQDEHMLA LEYALLAESD
360 370 380 390 400
GNARAFLKQN NQPMIVKNCS IMVRHYKTAL VAKMSISQYV NKRCLDHGEA
410 420 430 440 450
DENSWRGIVH FLRYQGQEFL PFMCKMHNFL HHRPKKSTLV LCGPSDTGKS
460 470 480 490 500
YFANGLNKFL DGHVLSFVSN GSHFWLSPLR GARCCLIDDA TLTFWRYADQ
510 520 530 540 550
NMRALLDGYE ISIDAKHRNP MQTRAPPLII TTNEDIMRLD EFKYLQTRTM
560 570 580 590 600
YVYFNKPFPL KGNGQPLYYI DGYTWNSFFR KFWRHLNLKD PEDESDGETP
610
GTIRLYTRAD TDTI
Length:614
Mass (Da):70,338
Last modified:August 1, 1992 - v1
Checksum:i82EFBA0B2A9F01F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56147 Genomic DNA Translation: CAA39614.1
PIRiC43550 W1WL41
RefSeqiNP_040287.1, NC_001354.1

Genome annotation databases

GeneIDi1489277
KEGGivg:1489277

Entry informationi

Entry nameiVE1_HPV41
AccessioniPrimary (citable) accession number: P27551
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 20, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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