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Protein

CD40 ligand

Gene

Cd40lg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).By similarity1 Publication
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Names & Taxonomyi

Protein namesi
Recommended name:
CD40 ligand
Short name:
CD40-L
Alternative name(s):
T-cell antigen Gp39
TNF-related activation protein
Short name:
TRAP
Tumor necrosis factor ligand superfamily member 5
CD_antigen: CD154
Cleaved into the following 2 chains:
Gene namesi
Name:Cd40lg
Synonyms:Cd40l, Tnfsf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88337 Cd40lg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 46Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini47 – 260ExtracellularSequence analysisAdd BLAST214

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000344901 – 260CD40 ligand, membrane formAdd BLAST260
ChainiPRO_0000034491112 – 260CD40 ligand, soluble formBy similarityAdd BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi177 ↔ 2171 Publication
Glycosylationi239N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111 – 112CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP27548
PRIDEiP27548

PTM databases

iPTMnetiP27548
PhosphoSitePlusiP27548

Expressioni

Tissue specificityi

Specifically expressed on activated CD4+ T-lymphocytes.

Gene expression databases

BgeeiENSMUSG00000031132 Expressed in 24 organ(s), highest expression level in thymus
CleanExiMM_CD40LG
ExpressionAtlasiP27548 baseline and differential
GenevisibleiP27548 MM

Interactioni

Subunit structurei

Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity).1 PublicationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466

Structurei

3D structure databases

ProteinModelPortaliP27548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVYF Eukaryota
ENOG4111TET LUCA
GeneTreeiENSGT00510000048489
HOGENOMiHOG000111291
HOVERGENiHBG079629
InParanoidiP27548
KOiK03161
OMAiNCEEIRS
OrthoDBiEOG091G0I9G
PhylomeDBiP27548
TreeFamiTF332169

Family and domain databases

CDDicd00184 TNF, 1 hit
Gene3Di2.60.120.40, 1 hit
InterProiView protein in InterPro
IPR003263 CD40L
IPR021184 TNF_CS
IPR006052 TNF_dom
IPR008983 Tumour_necrosis_fac-like_dom
PANTHERiPTHR11471:SF5 PTHR11471:SF5, 1 hit
PfamiView protein in Pfam
PF00229 TNF, 1 hit
PIRSFiPIRSF016527 TNF_5, 1 hit
PRINTSiPR01702 CD40LIGAND
SMARTiView protein in SMART
SM00207 TNF, 1 hit
SUPFAMiSSF49842 SSF49842, 1 hit
PROSITEiView protein in PROSITE
PS00251 TNF_1, 1 hit
PS50049 TNF_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27548-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL
60 70 80 90 100
DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL
110 120 130 140 150
NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN
160 170 180 190 200
LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS
210 220 230 240 250
ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV
260
GFSSFGLLKL
Length:260
Mass (Da):29,370
Last modified:August 14, 2001 - v2
Checksum:i7E1AC117473672AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198S → I in AAC13640 (PubMed:7520529).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA Translation: CAA46448.2
S71858 Genomic DNA Translation: AAC13639.1
S71861 Genomic DNA Translation: AAC13640.1
CCDSiCCDS30152.1
PIRiS21738
RefSeqiNP_035746.2, NM_011616.2
UniGeneiMm.4861

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132
GeneIDi21947
KEGGimmu:21947
UCSCiuc009thb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA Translation: CAA46448.2
S71858 Genomic DNA Translation: AAC13639.1
S71861 Genomic DNA Translation: AAC13640.1
CCDSiCCDS30152.1
PIRiS21738
RefSeqiNP_035746.2, NM_011616.2
UniGeneiMm.4861

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDAmodel-A/B/C115-260[»]
ProteinModelPortaliP27548
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466

PTM databases

iPTMnetiP27548
PhosphoSitePlusiP27548

Proteomic databases

PaxDbiP27548
PRIDEiP27548

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132
GeneIDi21947
KEGGimmu:21947
UCSCiuc009thb.1 mouse

Organism-specific databases

CTDi959
MGIiMGI:88337 Cd40lg

Phylogenomic databases

eggNOGiENOG410IVYF Eukaryota
ENOG4111TET LUCA
GeneTreeiENSGT00510000048489
HOGENOMiHOG000111291
HOVERGENiHBG079629
InParanoidiP27548
KOiK03161
OMAiNCEEIRS
OrthoDBiEOG091G0I9G
PhylomeDBiP27548
TreeFamiTF332169

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Miscellaneous databases

PROiPR:P27548
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031132 Expressed in 24 organ(s), highest expression level in thymus
CleanExiMM_CD40LG
ExpressionAtlasiP27548 baseline and differential
GenevisibleiP27548 MM

Family and domain databases

CDDicd00184 TNF, 1 hit
Gene3Di2.60.120.40, 1 hit
InterProiView protein in InterPro
IPR003263 CD40L
IPR021184 TNF_CS
IPR006052 TNF_dom
IPR008983 Tumour_necrosis_fac-like_dom
PANTHERiPTHR11471:SF5 PTHR11471:SF5, 1 hit
PfamiView protein in Pfam
PF00229 TNF, 1 hit
PIRSFiPIRSF016527 TNF_5, 1 hit
PRINTSiPR01702 CD40LIGAND
SMARTiView protein in SMART
SM00207 TNF, 1 hit
SUPFAMiSSF49842 SSF49842, 1 hit
PROSITEiView protein in PROSITE
PS00251 TNF_1, 1 hit
PS50049 TNF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCD40L_MOUSE
AccessioniPrimary (citable) accession number: P27548
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 14, 2001
Last modified: November 7, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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