Cd40lg

Functionⁱ

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).By similarity1 Publication

Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.By similarity

GO - Molecular functionⁱ

CD40 receptor binding Source: UniProtKB
cytokine activity Source: UniProtKB-KW
tumor necrosis factor receptor binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

activation of JUN kinase activity Source: UniProtKB
B cell differentiation
Source: MGI
Inferred from genetic interactionⁱ
- 14647274
B cell proliferation Source: UniProtKB
dendritic cell differentiation Source: MGI
immunoglobulin secretion
Source: MGI
Inferred from genetic interactionⁱ
- 15485634
inflammatory response Source: UniProtKB
isotype switching
Source: UniProtKB
Inferred from direct assayⁱ
- 7529792
negative regulation of apoptotic process Source: UniProtKB
platelet activation Source: UniProtKB
positive regulation of B cell proliferation Source: MGI
positive regulation of dendritic cell differentiation Source: MGI
positive regulation of endothelial cell apoptotic process Source: MGI
positive regulation of gene expression Source: MGI
positive regulation of immune response to tumor cell Source: MGI
positive regulation of interferon-gamma production Source: MGI
positive regulation of interleukin-10 production Source: UniProtKB
positive regulation of interleukin-12 production Source: MGI
positive regulation of interleukin-4 production Source: UniProtKB
positive regulation of MHC class II biosynthetic process Source: MGI
positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
positive regulation of T cell proliferation Source: UniProtKB
regulation of immunoglobulin secretion
Source: MGI
Inferred from direct assayⁱ
- 12958312
signal transduction
Source: UniProtKB
Non-traceable author statementⁱ
- 7529792

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Cytokine

Molecular function

Cytokine

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-5668541 TNFR2 non-canonical NF-kB pathway R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: CD40 ligand Short name: CD40-L Alternative name(s): T-cell antigen Gp39 TNF-related activation protein Short name: TRAP Tumor necrosis factor ligand superfamily member 5 CD_antigen: CD154 Cleaved into the following 2 chains: CD40 ligand, membrane form CD40 ligand, soluble form
Gene namesⁱ	Name:Cd40lg Synonyms:Cd40l, Tnfsf5
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:88337 Cd40lg

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 22	CytoplasmicSequence analysisAdd BLAST	22
Transmembraneⁱ	23 – 46	Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST	24
Topological domainⁱ	47 – 260	ExtracellularSequence analysisAdd BLAST	214

Keywords - Cellular componentⁱ

Cell membrane, Membrane, Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000034490	1 – 260	CD40 ligand, membrane formAdd BLAST		260
ChainⁱPRO_0000034491	112 – 260	CD40 ligand, soluble formBy similarityAdd BLAST		149

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Disulfide bondⁱ	177 ↔ 217	1 Publication
Glycosylationⁱ	239	N-linked (GlcNAc...) asparagineSequence analysis		1

Post-translational modificationⁱ

The soluble form derives from the membrane form by proteolytic processing.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	111 – 112	CleavageBy similarity		2

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbⁱ	P27548
PRIDEⁱ	P27548

PTM databases

iPTMnetⁱ	P27548
PhosphoSitePlusⁱ	P27548

Expressionⁱ

Tissue specificityⁱ

Specifically expressed on activated CD4+ T-lymphocytes.

Gene expression databases

Bgeeⁱ	ENSMUSG00000031132 Expressed in 24 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD40LG
ExpressionAtlasⁱ	P27548 baseline and differential
Genevisibleⁱ	P27548 MM

Interactionⁱ

Subunit structureⁱ

Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity).1 PublicationBy similarity

GO - Molecular functionⁱ

CD40 receptor binding Source: UniProtKB
cytokine activity Source: UniProtKB-KW
tumor necrosis factor receptor binding Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000033466

STRINGⁱ

10090.ENSMUSP00000033466

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P27548
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the tumor necrosis factor family.Curated

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IVYF Eukaryota ENOG4111TET LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000048489
HOGENOMⁱ	HOG000111291
HOVERGENⁱ	HBG079629
InParanoidⁱ	P27548
KEGG Orthology (KO) More...KOⁱ	K03161
OMAⁱ	NCEEIRS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0I9G
PhylomeDBⁱ	P27548
TreeFamⁱ	TF332169

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00184 TNF, 1 hit
Gene3Dⁱ	2.60.120.40, 1 hit
InterProⁱ	View protein in InterPro IPR003263 CD40L IPR021184 TNF_CS IPR006052 TNF_dom IPR008983 Tumour_necrosis_fac-like_dom
PANTHERⁱ	PTHR11471:SF5 PTHR11471:SF5, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00229 TNF, 1 hit
PIRSFⁱ	PIRSF016527 TNF_5, 1 hit
PRINTSⁱ	PR01702 CD40LIGAND
SMARTⁱ	View protein in SMART SM00207 TNF, 1 hit
SUPFAMⁱ	SSF49842 SSF49842, 1 hit
PROSITEⁱ	View protein in PROSITE PS00251 TNF_1, 1 hit PS50049 TNF_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P27548-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL 
        60         70         80         90        100
DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL 
       110        120        130        140        150
NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN 
       160        170        180        190        200
LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS 
       210        220        230        240        250
ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV 
       260
GFSSFGLLKL

Length:260

Mass (Da):29,370

Last modified:August 14, 2001 - v2

Checksum:ⁱ7E1AC117473672AD

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	198	S → I in AAC13640 (PubMed:7520529).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X65453 mRNA Translation: CAA46448.2 S71858 Genomic DNA Translation: AAC13639.1 S71861 Genomic DNA Translation: AAC13640.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30152.1
PIRⁱ	S21738
NCBI Reference Sequences More...RefSeqⁱ	NP_035746.2, NM_011616.2
UniGeneⁱ	Mm.4861

Genome annotation databases

Ensemblⁱ	ENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132
GeneIDⁱ	21947
KEGGⁱ	mmu:21947
UCSC genome browser More...UCSCⁱ	uc009thb.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P27548	CD40 ligand		MOUSE		260	UniRef100_P27548

Protein	Similar proteins		Species	Score	Length	Source
P27548	CD40 ligand		RAT		260	UniRef90_Q9Z2V2
CD40 ligand		MOUSE		260
UPI000A30CA4A		MUSPA		260
UPI000A3189EC		MUSCR		260
CD40 ligand		RAT		260

Protein	Similar proteins		Species	Score	Length	Source
P27548	CD40 ligand	)	HUMAN		261	UniRef50_P29965
CD40 ligand		AOTTR		261
CD40 ligand		CALJA		261
CD40 ligand		RAT		260
CD40 ligand		MACNE		240
+177

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X65453 mRNA Translation: CAA46448.2 S71858 Genomic DNA Translation: AAC13639.1 S71861 Genomic DNA Translation: AAC13640.1
CCDSⁱ	CCDS30152.1
PIRⁱ	S21738
RefSeqⁱ	NP_035746.2, NM_011616.2
UniGeneⁱ	Mm.4861

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1CDA	model	-	A/B/C	115-260	[»]
ProteinModelPortalⁱ	P27548
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000033466

STRINGⁱ

10090.ENSMUSP00000033466

PTM databases

iPTMnetⁱ	P27548
PhosphoSitePlusⁱ	P27548

Proteomic databases

PaxDbⁱ	P27548
PRIDEⁱ	P27548

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132
GeneIDⁱ	21947
KEGGⁱ	mmu:21947
UCSCⁱ	uc009thb.1 mouse

Organism-specific databases

CTDⁱ	959
MGIⁱ	MGI:88337 Cd40lg

Phylogenomic databases

eggNOGⁱ	ENOG410IVYF Eukaryota ENOG4111TET LUCA
GeneTreeⁱ	ENSGT00510000048489
HOGENOMⁱ	HOG000111291
HOVERGENⁱ	HBG079629
InParanoidⁱ	P27548
KOⁱ	K03161
OMAⁱ	NCEEIRS
OrthoDBⁱ	EOG091G0I9G
PhylomeDBⁱ	P27548
TreeFamⁱ	TF332169

Enzyme and pathway databases

Reactomeⁱ	R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell R-MMU-5668541 TNFR2 non-canonical NF-kB pathway R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Reactomeⁱ

R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-5668541 TNFR2 non-canonical NF-kB pathway
R-MMU-5676594 TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P27548
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031132 Expressed in 24 organ(s), highest expression level in thymus
CleanExⁱ	MM_CD40LG
ExpressionAtlasⁱ	P27548 baseline and differential
Genevisibleⁱ	P27548 MM

Family and domain databases

CDDⁱ	cd00184 TNF, 1 hit
Gene3Dⁱ	2.60.120.40, 1 hit
InterProⁱ	View protein in InterPro IPR003263 CD40L IPR021184 TNF_CS IPR006052 TNF_dom IPR008983 Tumour_necrosis_fac-like_dom
PANTHERⁱ	PTHR11471:SF5 PTHR11471:SF5, 1 hit
Pfamⁱ	View protein in Pfam PF00229 TNF, 1 hit
PIRSFⁱ	PIRSF016527 TNF_5, 1 hit
PRINTSⁱ	PR01702 CD40LIGAND
SMARTⁱ	View protein in SMART SM00207 TNF, 1 hit
SUPFAMⁱ	SSF49842 SSF49842, 1 hit
PROSITEⁱ	View protein in PROSITE PS00251 TNF_1, 1 hit PS50049 TNF_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CD40L_MOUSE
Accessionⁱ	P27548Primary (citable) accession number: P27548
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
	Last sequence update:	August 14, 2001
	Last modified:	November 7, 2018
	This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

UniProtKB

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UniProtKB - P27548 (CD40L_MOUSE)

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CD40 ligand

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Extracellular region or secreted

Extracellular region or secreted

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ