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UniProtKB - P27476 (NSR1_YEAST)
Protein
Nuclear localization sequence-binding protein
Gene
NSR1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in pre-rRNA processing. Specifically binds nuclear localization sequences. Candidate for a receptor at the nucleus that may be involved in both RNA and protein transport. Binds telomeric sequences of the type (TG[1-3])n in vitro.
Miscellaneous
Present with 77400 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- mRNA binding Source: SGD
- nuclear localization sequence binding Source: SGD
- RNA binding Source: SGD
- single-stranded telomeric DNA binding Source: SGD
GO - Biological processi
- maturation of SSU-rRNA Source: ComplexPortal
- ribosomal small subunit assembly Source: SGD
- rRNA processing Source: SGD
Keywordsi
Molecular function | DNA-binding, RNA-binding |
Biological process | rRNA processing, Stress response |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear localization sequence-binding proteinAlternative name(s): p67 |
Gene namesi | Name:NSR1 Ordered Locus Names:YGR159C ORF Names:G7001 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003391, NSR1 |
VEuPathDBi | FungiDB:YGR159C |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Mitochondrion
- mitochondrion Source: SGD
Nucleus
- nuclear envelope Source: SGD
- nucleolus Source: ComplexPortal
- nucleus Source: SGD
Other locations
- small-subunit processome Source: ComplexPortal
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081690 | 1 – 414 | Nuclear localization sequence-binding proteinAdd BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 353 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 362 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 366 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 375 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 379 | Asymmetric dimethylarginine1 Publication | 1 | |
Modified residuei | 382 | Asymmetric dimethylarginine1 Publication | 1 |
Post-translational modificationi
Methylated by HMT1, forming asymmetric dimethylarginines (DMA) within a domain referred to as an RGG box, made up of repeated Gly-Gly dipeptides interspersed with Arg and aromatic residues.1 Publication
Pyrophosphorylated by 5-diphosphoinositol pentakisphosphate (5-IP7) (PubMed:15604408). Serine pyrophosphorylation is achieved by Mg2+-dependent, but enzyme independent transfer of a beta-phosphate from a inositol pyrophosphate to a pre-phosphorylated serine residue (PubMed:15604408, PubMed:17873058).2 Publications
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
MaxQBi | P27476 |
PaxDbi | P27476 |
PRIDEi | P27476 |
PTM databases
iPTMneti | P27476 |
Expressioni
Inductioni
In response to low temperature (By cold-shock).
Interactioni
Protein-protein interaction databases
BioGRIDi | 33407, 133 interactors |
ComplexPortali | CPX-1604, Small ribosomal subunit processome, variant 1 CPX-1607, Small ribosomal subunit processome, variant 2 CPX-1608, Small ribosomal subunit processome, variant 3 |
DIPi | DIP-5537N |
IntActi | P27476, 52 interactors |
MINTi | P27476 |
STRINGi | 4932.YGR159C |
Miscellaneous databases
RNActi | P27476, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 168 – 246 | RRM 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 267 – 345 | RRM 2PROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 172 | DisorderedSequence analysisAdd BLAST | 172 | |
Regioni | 244 – 264 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 336 – 414 | DisorderedSequence analysisAdd BLAST | 79 | |
Regioni | 353 – 384 | RGG-boxAdd BLAST | 32 | |
Regioni | 366 – 384 | RNA-binding RGG-boxBy similarityAdd BLAST | 19 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 28 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 29 – 74 | Polar residuesSequence analysisAdd BLAST | 46 | |
Compositional biasi | 75 – 89 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 127 – 146 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 153 – 167 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 393 – 414 | Polar residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the RRM GAR family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4210, Eukaryota |
GeneTreei | ENSGT00940000170607 |
HOGENOMi | CLU_026791_0_0_1 |
InParanoidi | P27476 |
OMAi | NYGQEAS |
Family and domain databases
CDDi | cd12447, RRM1_gar2, 1 hit cd12448, RRM2_gar2, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034272, Gar2_RRM1 IPR034276, Gar2_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
i Sequence
Sequence statusi: Complete.
P27476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKTTKVKGN KKEVKASKQA KEEKAKAVSS SSSESSSSSS SSSESESESE
60 70 80 90 100
SESESSSSSS SSDSESSSSS SSDSESEAET KKEESKDSSS SSSDSSSDEE
110 120 130 140 150
EEEEKEETKK EESKESSSSD SSSSSSSDSE SEKEESNDKK RKSEDAEEEE
160 170 180 190 200
DEESSNKKQK NEETEEPATI FVGRLSWSID DEWLKKEFEH IGGVIGARVI
210 220 230 240 250
YERGTDRSRG YGYVDFENKS YAEKAIQEMQ GKEIDGRPIN CDMSTSKPAG
260 270 280 290 300
NNDRAKKFGD TPSEPSDTLF LGNLSFNADR DAIFELFAKH GEVVSVRIPT
310 320 330 340 350
HPETEQPKGF GYVQFSNMED AKKALDALQG EYIDNRPVRL DFSSPRPNND
360 370 380 390 400
GGRGGSRGFG GRGGGRGGNR GFGGRGGARG GRGGFRPSGS GANTAPLGRS
410
RNTASFAGSK KTFD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57185 Genomic DNA Translation: CAA40472.1 X85807 Genomic DNA Translation: CAA59817.1 Z72944 Genomic DNA Translation: CAA97173.1 Z72946 Genomic DNA Translation: CAA97180.1 BK006941 Genomic DNA Translation: DAA08250.1 |
PIRi | A39205 |
RefSeqi | NP_011675.1, NM_001181288.1 |
Genome annotation databases
EnsemblFungii | YGR159C_mRNA; YGR159C; YGR159C |
GeneIDi | 853064 |
KEGGi | sce:YGR159C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57185 Genomic DNA Translation: CAA40472.1 X85807 Genomic DNA Translation: CAA59817.1 Z72944 Genomic DNA Translation: CAA97173.1 Z72946 Genomic DNA Translation: CAA97180.1 BK006941 Genomic DNA Translation: DAA08250.1 |
PIRi | A39205 |
RefSeqi | NP_011675.1, NM_001181288.1 |
3D structure databases
AlphaFoldDBi | P27476 |
SMRi | P27476 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33407, 133 interactors |
ComplexPortali | CPX-1604, Small ribosomal subunit processome, variant 1 CPX-1607, Small ribosomal subunit processome, variant 2 CPX-1608, Small ribosomal subunit processome, variant 3 |
DIPi | DIP-5537N |
IntActi | P27476, 52 interactors |
MINTi | P27476 |
STRINGi | 4932.YGR159C |
PTM databases
iPTMneti | P27476 |
Proteomic databases
MaxQBi | P27476 |
PaxDbi | P27476 |
PRIDEi | P27476 |
Genome annotation databases
EnsemblFungii | YGR159C_mRNA; YGR159C; YGR159C |
GeneIDi | 853064 |
KEGGi | sce:YGR159C |
Organism-specific databases
SGDi | S000003391, NSR1 |
VEuPathDBi | FungiDB:YGR159C |
Phylogenomic databases
eggNOGi | KOG4210, Eukaryota |
GeneTreei | ENSGT00940000170607 |
HOGENOMi | CLU_026791_0_0_1 |
InParanoidi | P27476 |
OMAi | NYGQEAS |
Miscellaneous databases
PROi | PR:P27476 |
RNActi | P27476, protein |
Family and domain databases
CDDi | cd12447, RRM1_gar2, 1 hit cd12448, RRM2_gar2, 1 hit |
Gene3Di | 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034272, Gar2_RRM1 IPR034276, Gar2_RRM2 IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00360, RRM, 2 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NSR1_YEAST | |
Accessioni | P27476Primary (citable) accession number: P27476 Secondary accession number(s): D6VUT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 192 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families