MARK3

Functionⁱ

Serine/threonine-protein kinase (PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714).3 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Activity regulationⁱ

Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-564.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	85	ATPPROSITE-ProRule annotation		1
Active siteⁱ	178	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	62 – 70	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 16980613
tau-protein kinase activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23666762

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

establishment of cell polarity Source: InterPro
MAPK cascade Source: Reactome
microtubule cytoskeleton organization Source: InterPro
negative regulation of hippo signaling
Source: UniProtKB
Inferred from direct assayⁱ
- 28087714
peptidyl-serine autophosphorylation
Source: ARUK-UCL
Inferred from direct assayⁱ
- 9543386
peptidyl-serine phosphorylation
Source: ARUK-UCL
Inferred from direct assayⁱ
- 9543386
positive regulation of protein binding
Source: ARUK-UCL
Inferred from direct assayⁱ
- 9543386
protein phosphorylation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23666762

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-5673000 RAF activation R-HSA-5674135 MAP2K and MAPK activation R-HSA-5675221 Negative regulation of MAPK pathway R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants R-HSA-6802948 Signaling by high-kinase activity BRAF mutants R-HSA-6802949 Signaling by RAS mutants R-HSA-6802952 Signaling by BRAF and RAF fusions R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
SignaLinkⁱ	P27448
SIGNORⁱ	P27448

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: MAP/microtubule affinity-regulating kinase 3 (EC:2.7.11.1) Alternative name(s): C-TAK1 Short name: cTAK1 Cdc25C-associated protein kinase 1 ELKL motif kinase 2 Short name: EMK-2 Protein kinase STK10 Ser/Thr protein kinase PAR-1 Short name: Par-1a Serine/threonine-protein kinase p78
Gene namesⁱ	Name:MARK3 Synonyms:CTAK1, EMK2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 14

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000075413.17
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:6897 MARK3
MIMⁱ	602678 gene
neXtProtⁱ	NX_P27448

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Cytoplasm, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	211	T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	4140
Open Targets More...OpenTargetsⁱ	ENSG00000075413
PharmGKBⁱ	PA30640

Chemistry databases

ChEMBLⁱ	CHEMBL5600
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2099

Polymorphism and mutation databases

BioMutaⁱ	MARK3
DMDMⁱ	341941142

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086304	1 – 753	MAP/microtubule affinity-regulating kinase 3Add BLAST		753

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	42	PhosphoserineCombined sources	1
Modified residueⁱ	211	Phosphothreonine; by LKB11 Publication	1
Modified residueⁱ	368	PhosphoserineBy similarity	1
Modified residueⁱ	374	PhosphoserineBy similarity	1
Modified residueⁱ	376	PhosphoserineCombined sources	1
Modified residueⁱ	380	PhosphoserineCombined sources	1
Modified residueⁱ	383	PhosphoserineCombined sources	1
Modified residueⁱ	400	PhosphoserineCombined sources	1
Modified residueⁱ	419	PhosphoserineCombined sources	1
Modified residueⁱ	469	PhosphoserineCombined sources	1
Modified residueⁱ	540	PhosphoserineCombined sources	1
Modified residueⁱ	543	PhosphoserineBy similarity	1
Modified residueⁱ	549	PhosphothreonineCombined sources	1
Modified residueⁱ	564	Phosphothreonine; by PKC/PRKCZBy similarity	1
Modified residueⁱ	583	PhosphoserineCombined sources	1
Modified residueⁱ	598	PhosphoserineCombined sources	1
Modified residueⁱ	601	PhosphoserineCombined sources	1
Modified residueⁱ	643	PhosphoserineCombined sources	1
Isoform 5 (identifier: P27448-6)
Modified residueⁱ	384	PhosphoserineCombined sources	1
Isoform 6 (identifier: P27448-7)
Modified residueⁱ	407	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.2 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	P27448
PaxDbⁱ	P27448
PeptideAtlas More...PeptideAtlasⁱ	P27448
PRIDEⁱ	P27448
ProteomicsDBⁱ	54386 54387 [P27448-2] 54388 [P27448-3] 54389 [P27448-4] 54390 [P27448-6] 54391 [P27448-7] 54392 [P27448-8]

PTM databases

iPTMnetⁱ	P27448
PhosphoSitePlusⁱ	P27448

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.

Gene expression databases

Bgeeⁱ	ENSG00000075413 Expressed in 228 organ(s), highest expression level in cerebellar hemisphere
CleanExⁱ	HS_MARK3
ExpressionAtlasⁱ	P27448 baseline and differential
Genevisibleⁱ	P27448 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA024652

HPAⁱ

HPA024652

Interactionⁱ

Subunit structureⁱ

Interacts with MAPT/TAU (PubMed:23666762). Interacts with DLG5 (via coiled-coil domain). Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5 (PubMed:28087714).2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ASB13	Q8WXK3	2	EBI-707595,EBI-707573
Ppm1l	Q8BHN0	3	EBI-707595,EBI-7970002	From Mus musculus.
SFN	P31947	2	EBI-707595,EBI-476295
TCEA2	Q15560	2	EBI-707595,EBI-710310
YWHAB	P31946	4	EBI-707595,EBI-359815
YWHAG	P61981	2	EBI-707595,EBI-359832
YWHAH	Q04917	4	EBI-707595,EBI-306940
YWHAZ	P63104	10	EBI-707595,EBI-347088

Protein-protein interaction databases

BioGridⁱ	110310, 67 interactors
Database of interacting proteins More...DIPⁱ	DIP-34637N
IntActⁱ	P27448, 55 interactors
Molecular INTeraction database More...MINTⁱ	P27448
STRINGⁱ	9606.ENSP00000411397

Chemistry databases

BindingDBⁱ	P27448

BindingDBⁱ

P27448

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	56 – 64	Combined sources	9
Beta strandⁱ	66 – 75	Combined sources	10
Turnⁱ	76 – 78	Combined sources	3
Beta strandⁱ	81 – 88	Combined sources	8
Helixⁱ	89 – 91	Combined sources	3
Helixⁱ	94 – 109	Combined sources	16
Beta strandⁱ	118 – 123	Combined sources	6
Beta strandⁱ	125 – 132	Combined sources	8
Helixⁱ	140 – 147	Combined sources	8
Helixⁱ	152 – 171	Combined sources	20
Helixⁱ	181 – 183	Combined sources	3
Beta strandⁱ	184 – 186	Combined sources	3
Beta strandⁱ	192 – 194	Combined sources	3
Helixⁱ	201 – 203	Combined sources	3
Beta strandⁱ	204 – 206	Combined sources	3
Helixⁱ	208 – 210	Combined sources	3
Beta strandⁱ	213 – 215	Combined sources	3
Helixⁱ	216 – 218	Combined sources	3
Helixⁱ	221 – 225	Combined sources	5
Helixⁱ	232 – 248	Combined sources	17
Helixⁱ	258 – 267	Combined sources	10
Helixⁱ	278 – 287	Combined sources	10
Turnⁱ	292 – 294	Combined sources	3
Helixⁱ	298 – 301	Combined sources	4
Turnⁱ	305 – 310	Combined sources	6
Helixⁱ	329 – 337	Combined sources	9
Helixⁱ	342 – 350	Combined sources	9
Helixⁱ	356 – 364	Combined sources	9

Show more details

3D structure databases

ProteinModelPortalⁱ	P27448
SMRⁱ	P27448
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P27448

EvolutionaryTraceⁱ

P27448

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	56 – 307	Protein kinasePROSITE-ProRule annotationAdd BLAST	252
Domainⁱ	326 – 365	UBAPROSITE-ProRule annotationAdd BLAST	40
Domainⁱ	704 – 753	KA1PROSITE-ProRule annotationAdd BLAST	50

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0586 Eukaryota ENOG410XNQ0 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140806
HOGENOMⁱ	HOG000233025
HOVERGENⁱ	HBG052453
InParanoidⁱ	P27448
KEGG Orthology (KO) More...KOⁱ	K08798
TreeFamⁱ	TF315213

Family and domain databases

InterProⁱ	View protein in InterPro IPR028375 KA1/Ssp2_C IPR001772 KA1_dom IPR011009 Kinase-like_dom_sf IPR033624 MARK/par1 IPR033628 MARK3 IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS IPR015940 UBA
PANTHERⁱ	PTHR24346 PTHR24346, 1 hit PTHR24346:SF1 PTHR24346:SF1, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02149 KA1, 1 hit PF00069 Pkinase, 1 hit PF00627 UBA, 1 hit
SMARTⁱ	View protein in SMART SM00220 S_TKc, 1 hit SM00165 UBA, 1 hit
SUPFAMⁱ	SSF103243 SSF103243, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS50032 KA1, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit PS50030 UBA, 1 hit

Sequences (7+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 7 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 7 described isoforms and 7 potential isoforms that are computationally mapped.ⁱShow all

Isoform 1 (identifier: P27448-5) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 
       210        220        230        240        250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 
       260        270        280        290        300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 
       310        320        330        340        350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 
       360        370        380        390        400
KMKYDEITAT YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS 
       410        420        430        440        450
PHHKVQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI 
       460        470        480        490        500
SSRKSSGSAV GGKGIAPASP MLGNASNPNK ADIPERKKSS TVPSSNTASG 
       510        520        530        540        550
GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS THSISSAATP 
       560        570        580        590        600
DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 
       610        620        630        640        650
STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSAEQKDEN 
       660        670        680        690        700
KEAKPRSLRF TWSMKTTSSM DPGDMMREIR KVLDANNCDY EQRERFLLFC 
       710        720        730        740        750
VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF KNIASKIANE 

LKL

Length:753

Mass (Da):84,429

Last modified:March 28, 2018 - v5

Checksum:ⁱB50B3C145E896B0B

GO

Isoform 2 (identifier: P27448-2) [UniParc]FASTA Add to basket

Also known as: CTAK75a

The sequence of this isoform differs from the canonical sequence as follows:
161-161: Q → QGCQAGQTIKVQVSFDLLSLMFTF
615-638: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QGCQAGQTIK VQVSFDLLSL MFTFIVSAVQ YCHQKRIVHR 
       210        220        230        240        250
DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP YAAPELFQGK 
       260        270        280        290        300
KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 
       310        320        330        340        350
TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS 
       360        370        380        390        400
DQKRIDIMVG MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSELDASDSS 
       410        420        430        440        450
SSSNLSLAKV RPSSDLNNST GQSPHHKVQR SVSSSQKQRR YSDHAGPAIP 
       460        470        480        490        500
SVVAYPKRSQ TSTADSDLKE DGISSRKSSG SAVGGKGIAP ASPMLGNASN 
       510        520        530        540        550
PNKADIPERK KSSTVPSSNT ASGGMTRRNT YVCSERTTAD RHSVIQNGKE 
       560        570        580        590        600
NSTIPDQRTP VASTHSISSA ATPDRIRFPR GTASRSTFHG QPRERRTATY 
       610        620        630        640        650
NGPPASPSLS HEATPLSQTR SRGSTNLFSK LTSKLTRSRN VSAEQKDENK 
       660        670        680        690        700
EAKPRSLRFT WSMKTTSSMD PGDMMREIRK VLDANNCDYE QRERFLLFCV 
       710        720        730        740        750
HGDGHAENLV QWEMEVCKLP RLSLNGVRFK RISGTSIAFK NIASKIANEL 

KL

Show »

Length:752

Mass (Da):83,955

Checksum:ⁱ4577DF508B021FAF

GO

Isoform 3 (identifier: P27448-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
615-638: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 
       210        220        230        240        250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 
       260        270        280        290        300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 
       310        320        330        340        350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 
       360        370        380        390        400
KMKYDEITAT YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS 
       410        420        430        440        450
PHHKVQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI 
       460        470        480        490        500
SSRKSSGSAV GGKGIAPASP MLGNASNPNK ADIPERKKSS TVPSSNTASG 
       510        520        530        540        550
GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS THSISSAATP 
       560        570        580        590        600
DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 
       610        620        630        640        650
STNLFSKLTS KLTRSRNVSA EQKDENKEAK PRSLRFTWSM KTTSSMDPGD 
       660        670        680        690        700
MMREIRKVLD ANNCDYEQRE RFLLFCVHGD GHAENLVQWE MEVCKLPRLS 
       710        720 
LNGVRFKRIS GTSIAFKNIA SKIANELKL

Show »

Length:729

Mass (Da):81,439

Checksum:ⁱ0B808DC1BC333C25

GO

Isoform 4 (identifier: P27448-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
615-623: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 
       210        220        230        240        250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 
       260        270        280        290        300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 
       310        320        330        340        350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 
       360        370        380        390        400
KMKYDEITAT YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS 
       410        420        430        440        450
PHHKVQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI 
       460        470        480        490        500
SSRKSSGSAV GGKGIAPASP MLGNASNPNK ADIPERKKSS TVPSSNTASG 
       510        520        530        540        550
GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS THSISSAATP 
       560        570        580        590        600
DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 
       610        620        630        640        650
STNLFSKLTS KLTRRLPTEY ERNGRYEGSS RNVSAEQKDE NKEAKPRSLR 
       660        670        680        690        700
FTWSMKTTSS MDPGDMMREI RKVLDANNCD YEQRERFLLF CVHGDGHAEN 
       710        720        730        740 
LVQWEMEVCK LPRLSLNGVR FKRISGTSIA FKNIASKIAN ELKL

Show »

Length:744

Mass (Da):83,248

Checksum:ⁱ6FB18E6E4B830D9F

GO

Isoform 5 (identifier: P27448-6) [UniParc]FASTA Add to basket

Also known as: p58

The sequence of this isoform differs from the canonical sequence as follows:
371-386: Missing.
615-638: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 
       210        220        230        240        250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 
       260        270        280        290        300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 
       310        320        330        340        350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 
       360        370        380        390        400
KMKYDEITAT YLLLGRKSSE VRPSSDLNNS TGQSPHHKVQ RSVSSSQKQR 
       410        420        430        440        450
RYSDHAGPAI PSVVAYPKRS QTSTADSDLK EDGISSRKSS GSAVGGKGIA 
       460        470        480        490        500
PASPMLGNAS NPNKADIPER KKSSTVPSSN TASGGMTRRN TYVCSERTTA 
       510        520        530        540        550
DRHSVIQNGK ENSTIPDQRT PVASTHSISS AATPDRIRFP RGTASRSTFH 
       560        570        580        590        600
GQPRERRTAT YNGPPASPSL SHEATPLSQT RSRGSTNLFS KLTSKLTRSR 
       610        620        630        640        650
NVSAEQKDEN KEAKPRSLRF TWSMKTTSSM DPGDMMREIR KVLDANNCDY 
       660        670        680        690        700
EQRERFLLFC VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF 
       710 
KNIASKIANE LKL

Show »

Length:713

Mass (Da):79,876

Checksum:ⁱ74C47366AA34FCF8

GO

Isoform 6 (identifier: P27448-7) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
161-161: Q → QGCQAGQTIKVQVSFDLLSLMFTF
371-386: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QGCQAGQTIK VQVSFDLLSL MFTFIVSAVQ YCHQKRIVHR 
       210        220        230        240        250
DLKAENLLLD ADMNIKIADF GFSNEFTVGG KLDTFCGSPP YAAPELFQGK 
       260        270        280        290        300
KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR GKYRIPFYMS 
       310        320        330        340        350
TDCENLLKRF LVLNPIKRGT LEQIMKDRWI NAGHEEDELK PFVEPELDIS 
       360        370        380        390        400
DQKRIDIMVG MGYSQEEIQE SLSKMKYDEI TATYLLLGRK SSEVRPSSDL 
       410        420        430        440        450
NNSTGQSPHH KVQRSVSSSQ KQRRYSDHAG PAIPSVVAYP KRSQTSTADS 
       460        470        480        490        500
DLKEDGISSR KSSGSAVGGK GIAPASPMLG NASNPNKADI PERKKSSTVP 
       510        520        530        540        550
SSNTASGGMT RRNTYVCSER TTADRHSVIQ NGKENSTIPD QRTPVASTHS 
       560        570        580        590        600
ISSAATPDRI RFPRGTASRS TFHGQPRERR TATYNGPPAS PSLSHEATPL 
       610        620        630        640        650
SQTRSRGSTN LFSKLTSKLT RRNMSFRFIK RLPTEYERNG RYEGSSRNVS 
       660        670        680        690        700
AEQKDENKEA KPRSLRFTWS MKTTSSMDPG DMMREIRKVL DANNCDYEQR 
       710        720        730        740        750
ERFLLFCVHG DGHAENLVQW EMEVCKLPRL SLNGVRFKRI SGTSIAFKNI 
       760
ASKIANELKL

Show »

Length:760

Mass (Da):85,381

Checksum:ⁱF5148E18A88C54B7

GO

Isoform 7 (identifier: P27448-8) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
179-257: Missing.
624-638: Missing.

« Hide

        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK ELRERVLRGK YRIPFYMSTD 
       210        220        230        240        250
CENLLKRFLV LNPIKRGTLE QIMKDRWINA GHEEDELKPF VEPELDISDQ 
       260        270        280        290        300
KRIDIMVGMG YSQEEIQESL SKMKYDEITA TYLLLGRKSS ELDASDSSSS 
       310        320        330        340        350
SNLSLAKVRP SSDLNNSTGQ SPHHKVQRSV SSSQKQRRYS DHAGPAIPSV 
       360        370        380        390        400
VAYPKRSQTS TADSDLKEDG ISSRKSSGSA VGGKGIAPAS PMLGNASNPN 
       410        420        430        440        450
KADIPERKKS STVPSSNTAS GGMTRRNTYV CSERTTADRH SVIQNGKENS 
       460        470        480        490        500
TIPDQRTPVA STHSISSAAT PDRIRFPRGT ASRSTFHGQP RERRTATYNG 
       510        520        530        540        550
PPASPSLSHE ATPLSQTRSR GSTNLFSKLT SKLTRRNMSF RFIKSRNVSA 
       560        570        580        590        600
EQKDENKEAK PRSLRFTWSM KTTSSMDPGD MMREIRKVLD ANNCDYEQRE 
       610        620        630        640        650
RFLLFCVHGD GHAENLVQWE MEVCKLPRLS LNGVRFKRIS GTSIAFKNIA 

SKIANELKL

Note: No experimental confirmation available.

Show »

Length:659

Mass (Da):74,091

Checksum:ⁱB002CF23F4B21A20

GO

Computationally mapped potential isoform sequencesⁱ

There are 7 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation
H0YIY6	H0YIY6_HUMAN	Non-specific serine/threonine prote... Non-specific serine/threonine protein kinase, EC 2.7.11.1	MARK3	505	Annotation score:
J3KNR0	J3KNR0_HUMAN	Non-specific serine/threonine prote... Non-specific serine/threonine protein kinase, EC 2.7.11.1	MARK3 hCG_25498	776	Annotation score:
A0A0A0MSZ1	A0A0A0MSZ1_HUMAN	Non-specific serine/threonine prote... Non-specific serine/threonine protein kinase, EC 2.7.11.1	MARK3 hCG_25498	752	Annotation score:
H0YNV4	H0YNV4_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 3	MARK3	178	Annotation score:
H0YKJ3	H0YKJ3_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 3	MARK3	129	Annotation score:
A2SY06	A2SY06_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 3	MARK3	331	Annotation score:
H0YKP9	H0YKP9_HUMAN	MAP/microtubule affinity-regulating... MAP/microtubule affinity-regulating kinase 3	MARK3	222	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	125	E → Q in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	139	E → K in AAL69982 (Ref. 4) Curated	1
Sequence conflictⁱ	139	E → K in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	149	R → K in AAD48007 (Ref. 2) Curated	1
Sequence conflictⁱ	425	A → G in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	456	S → T in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	516	A → D in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	603	N → T in AAA59991 (Ref. 5) Curated	1
Sequence conflictⁱ	645	E → K in AAA59991 (Ref. 5) Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_080189	410	S → F1 PublicationCorresponds to variant dbSNP:rs10137161Ensembl.	1
Natural variantⁱVAR_040765	429	V → A1 Publication	1
Natural variantⁱVAR_046763	443	S → G3 PublicationsCorresponds to variant dbSNP:rs56305318Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_041582	161	Q → QGCQAGQTIKVQVSFDLLSL MFTF in isoform 2 and isoform 6. 1 Publication	1
Alternative sequenceⁱVSP_043197	179 – 257	Missing in isoform 7. 1 PublicationAdd BLAST	79
Alternative sequenceⁱVSP_004943	371 – 386	Missing in isoform 5 and isoform 6. 2 PublicationsAdd BLAST	16
Alternative sequenceⁱVSP_004944	615 – 638	Missing in isoform 2, isoform 3 and isoform 5. 4 PublicationsAdd BLAST	24
Alternative sequenceⁱVSP_004945	615 – 623	Missing in isoform 4. 1 Publication	9
Alternative sequenceⁱVSP_043198	624 – 638	Missing in isoform 7. 1 PublicationAdd BLAST	15

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U64205 mRNA Translation: AAC15093.1 AF159295 mRNA Translation: AAD48007.1 AF387637 mRNA Translation: AAK82367.1 AF465413 mRNA Translation: AAL69982.1 M80359 mRNA Translation: AAA59991.1 BX161395 mRNA Translation: CAD61882.1 AL133367 Genomic DNA No translation available. KF456011 Genomic DNA No translation available. CH471061 Genomic DNA Translation: EAW81813.1 CH471061 Genomic DNA Translation: EAW81815.1 CH471061 Genomic DNA Translation: EAW81817.1 BC024773 mRNA Translation: AAH24773.1 AF170723 mRNA Translation: AAD51631.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS41993.1 [P27448-3] CCDS45165.1 [P27448-5] CCDS45166.1 [P27448-4] CCDS45167.1 [P27448-8] CCDS55947.1 [P27448-6]
PIRⁱ	S27966
NCBI Reference Sequences More...RefSeqⁱ	NP_001122390.2, NM_001128918.2 [P27448-5] NP_001122391.2, NM_001128919.2 [P27448-4] NP_001122392.2, NM_001128920.2 [P27448-6] NP_001122393.2, NM_001128921.2 [P27448-8] NP_002367.5, NM_002376.6 [P27448-3]
UniGeneⁱ	Hs.35828

Genome annotation databases

Ensemblⁱ	ENST00000216288; ENSP00000216288; ENSG00000075413 [P27448-6] ENST00000303622; ENSP00000303698; ENSG00000075413 [P27448-3] ENST00000416682; ENSP00000408092; ENSG00000075413 [P27448-2] ENST00000429436; ENSP00000411397; ENSG00000075413 [P27448-5] ENST00000440884; ENSP00000402104; ENSG00000075413 [P27448-8] ENST00000553942; ENSP00000450772; ENSG00000075413 [P27448-4]
GeneIDⁱ	4140
KEGGⁱ	hsa:4140
UCSC genome browser More...UCSCⁱ	uc001ymw.5 human [P27448-5] uc001ymy.5 human uc001ymz.5 human uc001yna.5 human

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P27448	Non-specific serine/threonine protein kinase		PANTR		753	UniRef100_P27448
UPI0003E5E7B0		HUMAN		622
P27448-3	Non-specific serine/threonine protein kinase		PANTR		729	UniRef100_K7BTU4
UPI0007DC7102		HUMAN		598

Protein	Similar proteins		Species	Score	Length	Source
P27448	MAP/microtubule affinity-regulating kinase 3	)	MOUSE		753	UniRef90_P27448
Non-specific serine/threonine protein kinase		PANTR		753
Non-specific serine/threonine protein kinase		CERAT		846
Non-specific serine/threonine protein kinase		MACNE		760
Non-specific serine/threonine protein kinase		MACMU		759
+416
P27448-2	Non-specific serine/threonine protein kinase		HUMAN		752	UniRef90_A0A0A0MSZ1
Non-specific serine/threonine protein kinase		PONAB		752
Non-specific serine/threonine protein kinase		PANTR		752
UPI0003C8A72B		CAMFR		623
Non-specific serine/threonine protein kinase		PANTR		603
P27448-3	Isoform 5 of MAP/microtubule affinity-regulating kinase 3		HUMAN		713	UniRef90_P27448-6
Non-specific serine/threonine protein kinase		MACMU		732
Non-specific serine/threonine protein kinase		MICSU		731
Uncharacterized protein (Fragment)		Micrurus lemniscatus lemniscatus		143
Uncharacterized protein		Amazon coral snake		112
+228
P27448-6	Non-specific serine/threonine protein kinase		MACMU		732	UniRef90_P27448-6
Non-specific serine/threonine protein kinase		MICSU		731
Uncharacterized protein (Fragment)		Micrurus lemniscatus lemniscatus		143
Uncharacterized protein		Amazon coral snake		112
Uncharacterized protein		Micrurus lemniscatus carvalhoi		112
+228
P27448-7	cDNA FLJ58507, highly similar to MAP/microtubule affinity-regulating kinase 3		HUMAN		462	UniRef90_P27448-7
Non-specific serine/threonine protein kinase		PANTR		618
UPI00057A80FA		CAMBA		631
UPI00059B3919		AILME		631
UPI000C29AF80		Ugandan red Colobus		621
+1
P27448-8	Non-specific serine/threonine protein kinase		ODORO		659	UniRef90_P27448-8
MAP/microtubule affinity-regulating kinase 3 isoform X6		Enhydra lutris kenyoni		659
Non-specific serine/threonine protein kinase		PANTR		659
Non-specific serine/threonine protein kinase		PANPA		659
Non-specific serine/threonine protein kinase		PROCO		659
+22

Protein	Similar proteins		Species	Score	Length	Source
P27448	Serine/threonine-protein kinase MARK1	)	HUMAN		795	UniRef50_Q9P0L2
Serine/threonine-protein kinase MARK1		MOUSE		795
Serine/threonine-protein kinase MARK1		RAT		793
Non-specific serine/threonine protein kinase		DIPOR		818
Non-specific serine/threonine protein kinase		MYODS		814
+1886
P27448-2	Isoform 3 of Serine/threonine-protein kinase MARK1		HUMAN		758	UniRef50_Q9P0L2-3
Non-specific serine/threonine protein kinase		MACFA		770
Non-specific serine/threonine protein kinase		MACNE		770
Non-specific serine/threonine protein kinase		MANLE		769
Non-specific serine/threonine protein kinase		PANTR		762
+186
P27448-3	Isoform 2 of MAP/microtubule affinity-regulating kinase 4		HUMAN		688	UniRef50_Q96L34-2
MAP/microtubule affinity-regulating kinase 4 isoform 2		CALJA		688
Microtubule affinity-regulating kinase 4		RAT		688
Microtubule affinity regulating kinase 4		MACMU		688
Microtubule affinity regulating kinase 4		CERAT		688
+727
P27448-6	Isoform 2 of MAP/microtubule affinity-regulating kinase 4		HUMAN		688	UniRef50_Q96L34-2
MAP/microtubule affinity-regulating kinase 4 isoform 2		CALJA		688
Microtubule affinity-regulating kinase 4		RAT		688
Microtubule affinity regulating kinase 4		MACMU		688
Microtubule affinity regulating kinase 4		CERAT		688
+727
P27448-7	Isoform 3 of Serine/threonine-protein kinase MARK1		HUMAN		758	UniRef50_Q9P0L2-3
Non-specific serine/threonine protein kinase		MACFA		770
Non-specific serine/threonine protein kinase		MACNE		770
Non-specific serine/threonine protein kinase		MANLE		769
Non-specific serine/threonine protein kinase		PANTR		762
+186
P27448-8	Non-specific serine/threonine protein kinase		PANTR		659	UniRef50_P27448-8
Non-specific serine/threonine protein kinase		GORGO		659
Non-specific serine/threonine protein kinase		RHIRO		659
Non-specific serine/threonine protein kinase		MACMU		659
Non-specific serine/threonine protein kinase		CERAT		659
+57

Entry informationⁱ

Entry nameⁱ	MARK3_HUMAN
Accessionⁱ	P27448Primary (citable) accession number: P27448 Secondary accession number(s): A0A0A0MQR8 Q9UN34
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
	Last sequence update:	March 28, 2018
	Last modified:	September 12, 2018
	This is version 189 of the entry and version 5 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniProtKB - P27448 (MARK3_HUMAN)

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MAP/microtubule affinity-regulating kinase 3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

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<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

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Cytosol

Extracellular region or secreted

Plasma Membrane

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

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Computationally mapped potential isoform sequencesi

Experimental Info

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ