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UniProtKB - P27448 (MARK3_HUMAN)

Entry version 204 (17 Jun 2020)
Sequence version 5 (28 Mar 2018)
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Protein

MAP/microtubule affinity-regulating kinase 3

Gene

MARK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serine/threonine-protein kinase (PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714).3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-564.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei85ATPPROSITE-ProRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei178Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi62 – 70ATPPROSITE-ProRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-5673000 RAF activation
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-9649948 Signaling downstream of RAS mutants

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		P27448

SIGNOR Signaling Network Open Resource

More...SIGNORi		P27448

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
MAP/microtubule affinity-regulating kinase 3 (EC:2.7.11.1)
Alternative name(s):
C-TAK1
Short name:
cTAK1
Cdc25C-associated protein kinase 1
ELKL motif kinase 2
Short name:
EMK-2
Protein kinase STK10
Ser/Thr protein kinase PAR-1
Short name:
Par-1a
Serine/threonine-protein kinase p78
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:MARK3
Synonyms:CTAK1, EMK2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 14

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000075413.17

Human Gene Nomenclature Database

More...HGNCi		HGNC:6897 MARK3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		602678 gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P27448

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Visual impairment and progressive phthisis bulbi (VIPB)1 Publication
The disease may be caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive, progressive disease characterized by poor vision at birth and development of bilateral phthisis bulbi by adulthood.
Related information in OMIM
Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_080778570R → G in VIPB. 1 PublicationCorresponds to variant dbSNP:rs376395495EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi211T → A: Prevents phosphorylation and activation by STK11/LKB1 complex. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNET

More...DisGeNETi		4140

MalaCards human disease database

More...MalaCardsi		MARK3
MIMi618283 phenotype

Open Targets

More...OpenTargetsi		ENSG00000075413

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA30640

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P27448 Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL5600

Drug and drug target database

More...DrugBanki		DB12010 Fostamatinib

DrugCentral

More...DrugCentrali		P27448

IUPHAR/BPS Guide to PHARMACOLOGY

More...GuidetoPHARMACOLOGYi		2099

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		MARK3

Domain mapping of disease mutations (DMDM)

More...DMDMi		341941142

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000863041 – 753MAP/microtubule affinity-regulating kinase 3Add BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei42PhosphoserineCombined sources1
Modified residuei211Phosphothreonine; by LKB11 Publication1
Modified residuei368PhosphoserineBy similarity1
Modified residuei374PhosphoserineBy similarity1
Modified residuei376PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1
Modified residuei383PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1
Modified residuei540PhosphoserineCombined sources1
Modified residuei543PhosphoserineBy similarity1
Modified residuei549PhosphothreonineCombined sources1
Modified residuei564Phosphothreonine; by PKC/PRKCZBy similarity1
Modified residuei583PhosphoserineCombined sources1
Modified residuei598PhosphoserineCombined sources1
Modified residuei601PhosphoserineCombined sources1
Modified residuei643PhosphoserineCombined sources1
Isoform 5 (identifier: P27448-6)
Modified residuei384PhosphoserineCombined sources1
Isoform 6 (identifier: P27448-7)
Modified residuei407PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		P27448

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P27448

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P27448

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P27448

PeptideAtlas

More...PeptideAtlasi		P27448

PRoteomics IDEntifications database

More...PRIDEi		P27448

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		54386 [P27448-5]
54387 [P27448-2]
54388 [P27448-3]
54389 [P27448-4]
54390 [P27448-6]
54391 [P27448-7]
54392 [P27448-8]

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P27448

MetOSite database of methionine sulfoxide sites

More...MetOSitei		P27448

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P27448

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitous.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000075413 Expressed in cerebellar hemisphere and 227 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P27448 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P27448 HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000075413 Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with MAPT/TAU (PubMed:23666762).

Interacts with DLG5 (via coiled-coil domain).

Interacts with STK3/MST2 and STK4/MST1 in the presence of DLG5 (PubMed:28087714).

Interacts with YWHAB, YWHAG, YWHAQ and YWHAZ (PubMed:16959763).

3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		110310, 95 interactors

Database of interacting proteins

More...DIPi		DIP-34637N

Protein interaction database and analysis system

More...IntActi		P27448, 70 interactors

Molecular INTeraction database

More...MINTi		P27448

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000411397

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P27448 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1753
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P27448

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P27448

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini56 – 307Protein kinasePROSITE-ProRule annotationAdd BLAST252
Domaini326 – 365UBAPROSITE-ProRule annotationAdd BLAST40
Domaini704 – 753KA1PROSITE-ProRule annotationAdd BLAST50

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0586 Eukaryota
ENOG410XNQ0 LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000154862

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P27448

KEGG Orthology (KO)

More...KOi		K08798

TreeFam database of animal gene trees

More...TreeFami		TF315213

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR028375 KA1/Ssp2_C
IPR001772 KA1_dom
IPR011009 Kinase-like_dom_sf
IPR033628 MARK3
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR015940 UBA

The PANTHER Classification System

More...PANTHERi		PTHR24346:SF1 PTHR24346:SF1, 2 hits

Pfam protein domain database

More...Pfami		View protein in Pfam
PF02149 KA1, 1 hit
PF00069 Pkinase, 1 hit
PF00627 UBA, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00220 S_TKc, 1 hit
SM00165 UBA, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103243 SSF103243, 1 hit
SSF56112 SSF56112, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50032 KA1, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50030 UBA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (7+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 7 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 7 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P27448-5) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ 
        60         70         80         90        100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL 
       110        120        130        140        150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM 
       160        170        180        190        200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS 
       210        220        230        240        250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS 
       260        270        280        290        300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ 
       310        320        330        340        350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS 
       360        370        380        390        400
KMKYDEITAT YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS 
       410        420        430        440        450
PHHKVQRSVS SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI 
       460        470        480        490        500
SSRKSSGSAV GGKGIAPASP MLGNASNPNK ADIPERKKSS TVPSSNTASG 
       510        520        530        540        550
GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS THSISSAATP 
       560        570        580        590        600
DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG 
       610        620        630        640        650
STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSAEQKDEN 
       660        670        680        690        700
KEAKPRSLRF TWSMKTTSSM DPGDMMREIR KVLDANNCDY EQRERFLLFC 
       710        720        730        740        750
VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF KNIASKIANE 

LKL
Length:753
Mass (Da):84,429
Last modified:March 28, 2018 - v5
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB50B3C145E896B0B
GO
Isoform 2 (identifier: P27448-2) [UniParc]FASTAAdd to basket
Also known as: CTAK75a

The sequence of this isoform differs from the canonical sequence as follows:
     161-161: Q → QGCQAGQTIKVQVSFDLLSLMFTF
     615-638: Missing.

Show »
Length:752
Mass (Da):83,955
Checksum:i4577DF508B021FAF
GO
Isoform 3 (identifier: P27448-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     615-638: Missing.

Show »
Length:729
Mass (Da):81,439
Checksum:i0B808DC1BC333C25
GO
Isoform 4 (identifier: P27448-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     615-623: Missing.

Show »
Length:744
Mass (Da):83,248
Checksum:i6FB18E6E4B830D9F
GO
Isoform 5 (identifier: P27448-6) [UniParc]FASTAAdd to basket
Also known as: p58

The sequence of this isoform differs from the canonical sequence as follows:
     371-386: Missing.
     615-638: Missing.

Show »
Length:713
Mass (Da):79,876
Checksum:i74C47366AA34FCF8
GO
Isoform 6 (identifier: P27448-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     161-161: Q → QGCQAGQTIKVQVSFDLLSLMFTF
     371-386: Missing.

Show »
Length:760
Mass (Da):85,381
Checksum:iF5148E18A88C54B7
GO
Isoform 7 (identifier: P27448-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     179-257: Missing.
     624-638: Missing.

Show »
Length:659
Mass (Da):74,091
Checksum:iB002CF23F4B21A20
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YIY6H0YIY6_HUMAN
Non-specific serine/threonine prote...
MARK3
505Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
J3KNR0J3KNR0_HUMAN
Non-specific serine/threonine prote...
MARK3 hCG_25498
776Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YNV4H0YNV4_HUMAN
MAP/microtubule affinity-regulating...
MARK3
178Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YKJ3H0YKJ3_HUMAN
MAP/microtubule affinity-regulating...
MARK3
129Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
H0YKP9H0YKP9_HUMAN
MAP/microtubule affinity-regulating...
MARK3
222Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A2SY06A2SY06_HUMAN
MAP/microtubule affinity-regulating...
MARK3
331Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti125E → Q in AAA59991 (Ref. 5) Curated1
Sequence conflicti139E → K in AAL69982 (Ref. 4) Curated1
Sequence conflicti139E → K in AAA59991 (Ref. 5) Curated1
Sequence conflicti149R → K in AAD48007 (Ref. 2) Curated1
Sequence conflicti425A → G in AAA59991 (Ref. 5) Curated1
Sequence conflicti456S → T in AAA59991 (Ref. 5) Curated1
Sequence conflicti516A → D in AAA59991 (Ref. 5) Curated1
Sequence conflicti603N → T in AAA59991 (Ref. 5) Curated1
Sequence conflicti645E → K in AAA59991 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_080189410S → F1 PublicationCorresponds to variant dbSNP:rs10137161Ensembl.1
Natural variantiVAR_040765429V → A1 Publication1
Natural variantiVAR_046763443S → G3 PublicationsCorresponds to variant dbSNP:rs56305318Ensembl.1
Natural variantiVAR_080778570R → G in VIPB. 1 PublicationCorresponds to variant dbSNP:rs376395495EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_041582161Q → QGCQAGQTIKVQVSFDLLSL MFTF in isoform 2 and isoform 6. 1 Publication1
Alternative sequenceiVSP_043197179 – 257Missing in isoform 7. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_004943371 – 386Missing in isoform 5 and isoform 6. 2 PublicationsAdd BLAST16
Alternative sequenceiVSP_004944615 – 638Missing in isoform 2, isoform 3 and isoform 5. 4 PublicationsAdd BLAST24
Alternative sequenceiVSP_004945615 – 623Missing in isoform 4. 1 Publication9
Alternative sequenceiVSP_043198624 – 638Missing in isoform 7. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U64205 mRNA Translation: AAC15093.1
AF159295 mRNA Translation: AAD48007.1
AF387637 mRNA Translation: AAK82367.1
AF465413 mRNA Translation: AAL69982.1
M80359 mRNA Translation: AAA59991.1
BX161395 mRNA Translation: CAD61882.1
AL133367 Genomic DNA No translation available.
KF456011 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81813.1
CH471061 Genomic DNA Translation: EAW81815.1
CH471061 Genomic DNA Translation: EAW81817.1
BC024773 mRNA Translation: AAH24773.1
AF170723 mRNA Translation: AAD51631.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS41993.1 [P27448-3]
CCDS45165.1 [P27448-5]
CCDS45166.1 [P27448-4]
CCDS45167.1 [P27448-8]
CCDS55947.1 [P27448-6]

Protein sequence database of the Protein Information Resource

More...PIRi		S27966

NCBI Reference Sequences

More...RefSeqi		NP_001122390.2, NM_001128918.2 [P27448-5]
NP_001122391.2, NM_001128919.2 [P27448-4]
NP_001122392.2, NM_001128920.2 [P27448-6]
NP_001122393.2, NM_001128921.2 [P27448-8]
NP_002367.5, NM_002376.6 [P27448-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000216288; ENSP00000216288; ENSG00000075413 [P27448-6]
ENST00000303622; ENSP00000303698; ENSG00000075413 [P27448-3]
ENST00000416682; ENSP00000408092; ENSG00000075413 [P27448-2]
ENST00000429436; ENSP00000411397; ENSG00000075413 [P27448-5]
ENST00000440884; ENSP00000402104; ENSG00000075413 [P27448-8]
ENST00000553942; ENSP00000450772; ENSG00000075413 [P27448-4]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		4140

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:4140

UCSC genome browser

More...UCSCi		uc001ymw.5 human [P27448-5]
uc001ymy.5 human
uc001ymz.5 human
uc001yna.5 human

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64205 mRNA Translation: AAC15093.1
AF159295 mRNA Translation: AAD48007.1
AF387637 mRNA Translation: AAK82367.1
AF465413 mRNA Translation: AAL69982.1
M80359 mRNA Translation: AAA59991.1
BX161395 mRNA Translation: CAD61882.1
AL133367 Genomic DNA No translation available.
KF456011 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81813.1
CH471061 Genomic DNA Translation: EAW81815.1
CH471061 Genomic DNA Translation: EAW81817.1
BC024773 mRNA Translation: AAH24773.1
AF170723 mRNA Translation: AAD51631.1
CCDSiCCDS41993.1 [P27448-3]
CCDS45165.1 [P27448-5]
CCDS45166.1 [P27448-4]
CCDS45167.1 [P27448-8]
CCDS55947.1 [P27448-6]
PIRiS27966
RefSeqiNP_001122390.2, NM_001128918.2 [P27448-5]
NP_001122391.2, NM_001128919.2 [P27448-4]
NP_001122392.2, NM_001128920.2 [P27448-6]
NP_001122393.2, NM_001128921.2 [P27448-8]
NP_002367.5, NM_002376.6 [P27448-3]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QNJX-ray2.70A/B48-370[»]
3FE3X-ray1.90A/B41-367[»]
SMRiP27448
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi110310, 95 interactors
DIPiDIP-34637N
IntActiP27448, 70 interactors
MINTiP27448
STRINGi9606.ENSP00000411397

Chemistry databases

ChEMBLiCHEMBL5600
DrugBankiDB12010 Fostamatinib
DrugCentraliP27448
GuidetoPHARMACOLOGYi2099

PTM databases

iPTMnetiP27448
MetOSiteiP27448
PhosphoSitePlusiP27448

Polymorphism and mutation databases

BioMutaiMARK3
DMDMi341941142

Proteomic databases

EPDiP27448
jPOSTiP27448
MassIVEiP27448
PaxDbiP27448
PeptideAtlasiP27448
PRIDEiP27448
ProteomicsDBi54386 [P27448-5]
54387 [P27448-2]
54388 [P27448-3]
54389 [P27448-4]
54390 [P27448-6]
54391 [P27448-7]
54392 [P27448-8]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		6515 450 antibodies

The DNASU plasmid repository

More...DNASUi		4140

Genome annotation databases

EnsembliENST00000216288; ENSP00000216288; ENSG00000075413 [P27448-6]
ENST00000303622; ENSP00000303698; ENSG00000075413 [P27448-3]
ENST00000416682; ENSP00000408092; ENSG00000075413 [P27448-2]
ENST00000429436; ENSP00000411397; ENSG00000075413 [P27448-5]
ENST00000440884; ENSP00000402104; ENSG00000075413 [P27448-8]
ENST00000553942; ENSP00000450772; ENSG00000075413 [P27448-4]
GeneIDi4140
KEGGihsa:4140
UCSCiuc001ymw.5 human [P27448-5]
uc001ymy.5 human
uc001ymz.5 human
uc001yna.5 human

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		4140
DisGeNETi4140
EuPathDBiHostDB:ENSG00000075413.17

GeneCards: human genes, protein and diseases

More...GeneCardsi		MARK3
HGNCiHGNC:6897 MARK3
HPAiENSG00000075413 Low tissue specificity
MalaCardsiMARK3
MIMi602678 gene
618283 phenotype
neXtProtiNX_P27448
OpenTargetsiENSG00000075413
PharmGKBiPA30640

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0586 Eukaryota
ENOG410XNQ0 LUCA
GeneTreeiENSGT00940000154862
InParanoidiP27448
KOiK08798
TreeFamiTF315213

Enzyme and pathway databases

ReactomeiR-HSA-5673000 RAF activation
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-9649948 Signaling downstream of RAS mutants
SignaLinkiP27448
SIGNORiP27448

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		4140 9 hits in 817 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		MARK3 human
EvolutionaryTraceiP27448

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		MARK3

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		4140
PharosiP27448 Tchem

Protein Ontology

More...PROi		PR:P27448
RNActiP27448 protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000075413 Expressed in cerebellar hemisphere and 227 other tissues
ExpressionAtlasiP27448 baseline and differential
GenevisibleiP27448 HS

Family and domain databases

InterProiView protein in InterPro
IPR028375 KA1/Ssp2_C
IPR001772 KA1_dom
IPR011009 Kinase-like_dom_sf
IPR033628 MARK3
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR015940 UBA
PANTHERiPTHR24346:SF1 PTHR24346:SF1, 2 hits
PfamiView protein in Pfam
PF02149 KA1, 1 hit
PF00069 Pkinase, 1 hit
PF00627 UBA, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00165 UBA, 1 hit
SUPFAMiSSF103243 SSF103243, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50032 KA1, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50030 UBA, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMARK3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P27448
Secondary accession number(s): A0A0A0MQR8
, A0A0A0MST9, A0A0A0MT23, O60219, Q86TT8, Q8TB41, Q8WX83, Q96RG1, Q9UMY9, Q9UN34
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: March 28, 2018
Last modified: June 17, 2020
This is version 204 of the entry and version 5 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  7. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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