UniProtKB - P27435 (TRYB1_RAT)
Protein
Tryptase
Gene
Tpsab1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity (By similarity).By similarity
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin. EC:3.4.21.59
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 72 | Charge relay systemBy similarity | 1 | |
Active sitei | 119 | Charge relay systemBy similarity | 1 | |
Active sitei | 222 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: RGD
- peptidase activity Source: RGD
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- extracellular matrix disassembly Source: RGD
- proteolysis Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Protein family/group databases
MEROPSi | S01.143 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptase (EC:3.4.21.59)Alternative name(s): Mast cell protease 7 Short name: rMCP-7 Tryptase alpha/beta-1 Tryptase, skin |
Gene namesi | Name:Tpsab1 Synonyms:Mcp7, Mcpt7, Tpsb1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3066, Tpsab1 |
Subcellular locationi
Extracellular region or secreted
Note: Released from the secretory granules upon mast cell activation.
Extracellular region or secreted
- extracellular space Source: RGD
Lysosome
- mast cell granule Source: RGD
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
PropeptideiPRO_0000027496 | 19 – 28 | Activation peptide2 Publications | 10 | |
ChainiPRO_0000027497 | 29 – 273 | TryptaseAdd BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 49 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 57 ↔ 73 | PROSITE-ProRule annotation | ||
Disulfide bondi | 153 ↔ 228 | PROSITE-ProRule annotation | ||
Disulfide bondi | 186 ↔ 209 | PROSITE-ProRule annotation | ||
Disulfide bondi | 218 ↔ 246 | PROSITE-ProRule annotation |
Post-translational modificationi
Glycosylated.Curated
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P27435 |
PTM databases
GlyGeni | P27435, 1 site |
Expressioni
Tissue specificityi
Mast cells.
Interactioni
Subunit structurei
Homotetramer.
GO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025095 |
Chemistry databases
BindingDBi | P27435 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 270 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 242 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | P27435 |
OMAi | NQLCDAE |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P27435 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P27435-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKLLLLTLP LLSSLVHAAP SLAMPREGIV GGQEASGNKW PWQVSLRVND
60 70 80 90 100
TYWMHFCGGS LIHPQWVLTA AHCVGPNKAD PNKLRVQLRK QYLYYHDHLL
110 120 130 140 150
TVSQIISHPD FYIAQDGADI ALLKLTNPVN ITSNVHTVSL PPASETFPSG
160 170 180 190 200
TLCWVTGWGN INNDVSLPPP FPLEEVQVPI VENRLCDLKY HKGLNTGDNV
210 220 230 240 250
HIVRDDMLCA GNEGHDSCQG DSGGPLVCKV EDTWLQAGVV SWGEGCAQPN
260 270
RPGIYTRVTY YLDWIYRYVP KYF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3V8E5 | G3V8E5_RAT | Tryptase | Tpsab1 rCG_34518 | 310 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | W → V AA sequence (PubMed:1314562).Curated | 1 | |
Sequence conflicti | 49 – 51 | NDT → WLP AA sequence (PubMed:1314562).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67910 mRNA Translation: AAB48263.1 |
PIRi | A23698 S21275 |
RefSeqi | NP_062195.2, NM_019322.2 |
Genome annotation databases
GeneIDi | 54271 |
KEGGi | rno:54271 |
UCSCi | RGD:3066, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U67910 mRNA Translation: AAB48263.1 |
PIRi | A23698 S21275 |
RefSeqi | NP_062195.2, NM_019322.2 |
3D structure databases
SMRi | P27435 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000025095 |
Chemistry databases
BindingDBi | P27435 |
ChEMBLi | CHEMBL3320 |
Protein family/group databases
MEROPSi | S01.143 |
PTM databases
GlyGeni | P27435, 1 site |
Proteomic databases
PaxDbi | P27435 |
Genome annotation databases
GeneIDi | 54271 |
KEGGi | rno:54271 |
UCSCi | RGD:3066, rat |
Organism-specific databases
CTDi | 7177 |
RGDi | 3066, Tpsab1 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | P27435 |
OMAi | NQLCDAE |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P27435 |
Miscellaneous databases
PROi | PR:P27435 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRYB1_RAT | |
Accessioni | P27435Primary (citable) accession number: P27435 Secondary accession number(s): P27436 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families