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Protein

3 beta-hydroxysteroid dehydrogenase type 5

Gene

Hsd3b5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD V can only catalyze the conversion of dihydrotestosterone to 5 alpha-androstanediol in the presence of the cofactor NADPH. Exhibits only 3-ketosteroid reductase activity in the presence of NADPH and does not function as an isomerase.

Catalytic activityi

3-beta-hydroxy-Delta5-steroid + NADP+ = 3-oxo-Delta5-steroid + NADPH.

Pathwayi: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei155Proton acceptorBy similarity1
Binding sitei159NADBy similarity1

GO - Molecular functioni

  • 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: RGD
  • NAD binding Source: RGD
  • steroid binding Source: RGD
  • steroid dehydrogenase activity Source: RGD

GO - Biological processi

  • progesterone metabolic process Source: RGD
  • regulation of steroid biosynthetic process Source: RGD
  • steroid biosynthetic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
Biological processSteroidogenesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00062

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase type 5
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase type V
Short name:
3-beta-HSD V
3-beta-hydroxy-5-ene steroid dehydrogenase
NADPH-dependent 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.-)
Progesterone reductase
Gene namesi
Name:Hsd3b5
Synonyms:Hsd3b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2838 Hsd3b5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877891 – 3733 beta-hydroxysteroid dehydrogenase type 5Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei350N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP27364
PRIDEiP27364

PTM databases

CarbonylDBiP27364
iPTMnetiP27364
PhosphoSitePlusiP27364

Expressioni

Tissue specificityi

Expressed predominantly in male liver.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026290

Structurei

3D structure databases

ProteinModelPortaliP27364
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1430 Eukaryota
COG0451 LUCA
HOGENOMiHOG000167989
HOVERGENiHBG000014
InParanoidiP27364
KOiK00070
PhylomeDBiP27364
TreeFamiTF343138

Family and domain databases

InterProiView protein in InterPro
IPR002225 3Beta_OHSteriod_DH/Estase
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01073 3Beta_HSD, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits

Sequencei

Sequence statusi: Complete.

P27364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFLGQRIV QMLVQEKELQ EVRVLYRTFS PKHKEELSKL
60 70 80 90 100
QTKAKVTVLR GDIVDAQFLR RACQGMSVII HTAAALDIAG FLPRQTILDV
110 120 130 140 150
NVKGTQLLLD ACVEASVPAF IYSSSTGVAG PNSYKETILN DREEEHREST
160 170 180 190 200
WSNPYPYSKR MAEKAVLAAN GSILKNGGTF HTCALRLPFI YGEESQIIST
210 220 230 240 250
MVNRALKNNS IIKRHATFSI ANPVYVGNAA WAHILAARGL RDPEKSQSIQ
260 270 280 290 300
GQFYYISDDT PHQSYDDLNY TLSKEWGFCL DSSWSLPLPL LYWLAFLLET
310 320 330 340 350
VSFLLRPFYN YRPPFNRFMV TILNSVFTIS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):42,206
Last modified:January 23, 2007 - v3
Checksum:iB96538F2EF056935
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180F → L in AAH92571 (PubMed:15489334).Curated1
Sequence conflicti204R → T in AAH92571 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67465 mRNA Translation: AAA41352.1
BC092571 mRNA Translation: AAH92571.1
PIRiA37404
RefSeqiNP_036716.1, NM_012584.1
UniGeneiRn.34902

Genome annotation databases

GeneIDi24470
KEGGirno:24470
UCSCiRGD:2838 rat

Similar proteinsi

Entry informationi

Entry namei3BHS5_RAT
AccessioniPrimary (citable) accession number: P27364
Secondary accession number(s): Q569C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 139 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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