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Protein

DNA polymerase epsilon subunit C

Gene

DPB3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.1 Publication

Miscellaneous

In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 784 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • error-prone translesion synthesis Source: SGD
  • heterochromatin organization involved in chromatin silencing Source: SGD
  • leading strand elongation Source: SGD
  • nucleic acid phosphodiester bond hydrolysis Source: GOC
  • replicative senescence Source: SGD

Keywordsi

Molecular functionDNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29198-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit C (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit C
Gene namesi
Name:DPB3
Ordered Locus Names:YBR278W
ORF Names:YBR2015
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR278W
SGDiS000000482 DPB3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083501 – 201DNA polymerase epsilon subunit CAdd BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186PhosphoserineCombined sources1
Modified residuei188PhosphoserineCombined sources1
Modified residuei189PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27344
PaxDbiP27344
PRIDEiP27344

PTM databases

iPTMnetiP27344

Interactioni

Subunit structurei

DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32972, 298 interactors
ComplexPortaliCPX-2110 DNA polymerase epsilon complex
DIPiDIP-4803N
IntActiP27344, 24 interactors
MINTiP27344
STRINGi4932.YBR278W

Structurei

3D structure databases

ProteinModelPortaliP27344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi120 – 154Asp/Glu-rich (acidic)Add BLAST35

Phylogenomic databases

InParanoidiP27344
KOiK03506
OMAiDYIFLED
OrthoDBiEOG092C51B1

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

P27344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLVKEKAP VFPISKVKKI AKCDPEYVIT SNVAISATAF AAELFVQNLV
60 70 80 90 100
EESLVLAQLN SKGKTSLRLS LNSIEECVEK RDNFRFLEDA IKQLKKNSAL
110 120 130 140 150
DKKRELNMQP GRSDQEVVIE EPELHEDDGV EEEEEEDEVS EEEEPVHNEE
160 170 180 190 200
LLDDSKDQQN DKSTRSVASL LSRFQYKSAL DVGEHSDSSD IEVDHTKSTD

P
Length:201
Mass (Da):22,665
Last modified:October 1, 1994 - v2
Checksum:i288913917D49C7F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196T → M in CAA41403 (PubMed:1923754).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58500 Genomic DNA Translation: CAA41403.1
X76053 Genomic DNA Translation: CAA53641.1
Z36146 Genomic DNA Translation: CAA85242.1
BK006936 Genomic DNA Translation: DAA07393.1
PIRiS44540
RefSeqiNP_009837.1, NM_001178626.1

Genome annotation databases

EnsemblFungiiYBR278W; YBR278W; YBR278W
GeneIDi852580
KEGGisce:YBR278W

Similar proteinsi

Entry informationi

Entry nameiDPB3_YEAST
AccessioniPrimary (citable) accession number: P27344
Secondary accession number(s): D6VQS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1994
Last modified: July 18, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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