UniProtKB - P27306 (STHA_ECOLI)
Protein
Soluble pyridine nucleotide transhydrogenase
Gene
sthA
Organism
Escherichia coli (strain K12)
Status
Functioni
Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation.
Catalytic activityi
- EC:1.6.1.1
Cofactori
FADNote: Binds 1 FAD per subunit.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 45 | FADBy similarity | 10 |
GO - Molecular functioni
- dihydrolipoyl dehydrogenase activity Source: GO_Central
- flavin adenine dinucleotide binding Source: EcoCyc
- identical protein binding Source: EcoCyc
- NAD(P)+ transhydrogenase (B-specific) activity Source: UniProtKB-UniRule
- NAD(P)+ transhydrogenase activity Source: EcoCyc
GO - Biological processi
- cell redox homeostasis Source: EcoCyc
- NADP metabolic process Source: UniProtKB-UniRule
- oxidation-reduction process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, NAD, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:UDHA-MONOMER MetaCyc:UDHA-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Soluble pyridine nucleotide transhydrogenase (EC:1.6.1.1)Short name: STH Alternative name(s): NAD(P)(+) transhydrogenase [B-specific] |
Gene namesi | Name:sthA Synonyms:sth, udhA Ordered Locus Names:b3962, JW5551 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000068061 | 2 – 466 | Soluble pyridine nucleotide transhydrogenaseAdd BLAST | 465 |
Proteomic databases
jPOSTi | P27306 |
PaxDbi | P27306 |
PRIDEi | P27306 |
Interactioni
Subunit structurei
Homooligomer; probable homooctamer.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263449, 8 interactors 852757, 1 interactor |
IntActi | P27306, 1 interactor |
STRINGi | 511145.b3962 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.Curated
Phylogenomic databases
eggNOGi | COG1249, Bacteria |
HOGENOMi | CLU_016755_0_0_6 |
InParanoidi | P27306 |
PhylomeDBi | P27306 |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
HAMAPi | MF_00247, SthA, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR001100, Pyr_nuc-diS_OxRdtase IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR022962, STH |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
PIRSFi | PIRSF000350, Mercury_reductase_MerA, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P27306-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT
60 70 80 90 100
IPSKALRHAV SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM
110 120 130 140 150
RQGFYERNHC EILQGNARFV DEHTLALDCP DGSVETLTAE KFVIACGSRP
160 170 180 190 200
YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL IYGAGVIGCE YASIFRGMDV
210 220 230 240 250
KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY EKIEGCDDGV
260 270 280 290 300
IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ
310 320 330 340 350
TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI
360 370 380 390 400
YTIPEISSVG KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF
410 420 430 440 450
HRETKEILGI HCFGERAAEI IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM
460
AEAYRVAALN GLNRLF
Sequence cautioni
The sequence AAC43068 differs from that shown. Reason: Frameshift.Curated
The sequence CAA46822 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 | I → L in CAA46822 (PubMed:1447162).Curated | 1 | |
Sequence conflicti | 118 | R → L in CAA46822 (PubMed:1447162).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43068.1 Frameshift. U00096 Genomic DNA Translation: AAC76944.2 AP009048 Genomic DNA Translation: BAE77349.1 X66026 Genomic DNA Translation: CAA46822.1 Frameshift. X16531 Genomic DNA No translation available. |
PIRi | E65203 |
RefSeqi | NP_418397.2, NC_000913.3 WP_001120810.1, NZ_STEB01000037.1 |
Genome annotation databases
EnsemblBacteriai | AAC76944; AAC76944; b3962 BAE77349; BAE77349; BAE77349 |
GeneIDi | 49586753 948461 |
KEGGi | ecj:JW5551 eco:b3962 |
PATRICi | fig|1411691.4.peg.2743 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43068.1 Frameshift. U00096 Genomic DNA Translation: AAC76944.2 AP009048 Genomic DNA Translation: BAE77349.1 X66026 Genomic DNA Translation: CAA46822.1 Frameshift. X16531 Genomic DNA No translation available. |
PIRi | E65203 |
RefSeqi | NP_418397.2, NC_000913.3 WP_001120810.1, NZ_STEB01000037.1 |
3D structure databases
SMRi | P27306 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263449, 8 interactors 852757, 1 interactor |
IntActi | P27306, 1 interactor |
STRINGi | 511145.b3962 |
Proteomic databases
jPOSTi | P27306 |
PaxDbi | P27306 |
PRIDEi | P27306 |
Genome annotation databases
EnsemblBacteriai | AAC76944; AAC76944; b3962 BAE77349; BAE77349; BAE77349 |
GeneIDi | 49586753 948461 |
KEGGi | ecj:JW5551 eco:b3962 |
PATRICi | fig|1411691.4.peg.2743 |
Organism-specific databases
EchoBASEi | EB1398 |
Phylogenomic databases
eggNOGi | COG1249, Bacteria |
HOGENOMi | CLU_016755_0_0_6 |
InParanoidi | P27306 |
PhylomeDBi | P27306 |
Enzyme and pathway databases
BioCyci | EcoCyc:UDHA-MONOMER MetaCyc:UDHA-MONOMER |
Miscellaneous databases
PROi | PR:P27306 |
Family and domain databases
Gene3Di | 3.30.390.30, 1 hit 3.50.50.60, 2 hits |
HAMAPi | MF_00247, SthA, 1 hit |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR023753, FAD/NAD-binding_dom IPR016156, FAD/NAD-linked_Rdtase_dimer_sf IPR001100, Pyr_nuc-diS_OxRdtase IPR004099, Pyr_nucl-diS_OxRdtase_dimer IPR022962, STH |
Pfami | View protein in Pfam PF07992, Pyr_redox_2, 1 hit PF02852, Pyr_redox_dim, 1 hit |
PIRSFi | PIRSF000350, Mercury_reductase_MerA, 1 hit |
SUPFAMi | SSF51905, SSF51905, 1 hit SSF55424, SSF55424, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STHA_ECOLI | |
Accessioni | P27306Primary (citable) accession number: P27306 Secondary accession number(s): Q2M8Q7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families