UniProtKB - P27303 (EMRA_ECOLI)
Protein
Multidrug export protein EmrA
Gene
emrA
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics such as CCCP, FCCP, 2,4-dinitrophenol and nalidixic acid. EmrA is a drug-binding protein that provides a physical link between EmrB and TolC.2 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
- xenobiotic transmembrane transporter activity Source: InterPro
GO - Biological processi
- response to antibiotic Source: EcoCyc
- response to toxic substance Source: EcoCyc
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11354-MONOMER MetaCyc:EG11354-MONOMER |
Protein family/group databases
TCDBi | 8.A.1.1.1, the membrane fusion protein (mfp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug export protein EmrA |
Gene namesi | Name:emrA Ordered Locus Names:b2685, JW2660 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Single-pass membrane protein 2 Publications; Periplasmic side 2 Publications
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- intrinsic component of plasma membrane Source: EcoliWiki
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 24 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 25 – 45 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 46 – 390 | PeriplasmicSequence analysisAdd BLAST | 345 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201869 | 1 – 390 | Multidrug export protein EmrAAdd BLAST | 390 |
Proteomic databases
jPOSTi | P27303 |
PaxDbi | P27303 |
PRIDEi | P27303 |
Interactioni
Subunit structurei
Homodimer and homotrimer. Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.
Interacts with EmrB. EmrAB complex forms a dimer in vitro.
3 PublicationsBinary interactionsi
Hide detailsP27303
With | #Exp. | IntAct |
---|---|---|
itself | 3 | EBI-1119547,EBI-1119547 |
emrB [P0AEJ0] | 2 | EBI-1119547,EBI-21407519 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262946, 219 interactors 851498, 1 interactor |
ComplexPortali | CPX-4268, EmrAB-TolC multidrug efflux transport system |
DIPi | DIP-9502N |
IntActi | P27303, 4 interactors |
STRINGi | 511145.b2685 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 120 – 180 | Sequence analysisAdd BLAST | 61 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1566, Bacteria |
HOGENOMi | CLU_018816_15_0_6 |
InParanoidi | P27303 |
PhylomeDBi | P27303 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR005694, Efflux_pump_mem IPR032317, HlyD_D23 |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR00998, 8a0101, 1 hit |
i Sequence
Sequence statusi: Complete.
P27303-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSANAETQTP QQPVKKSGKR KRLLLLLTLL FIIIAVAIGI YWFLVLRHFE
60 70 80 90 100
ETDDAYVAGN QIQIMSQVSG SVTKVWADNT DFVKEGDVLV TLDPTDARQA
110 120 130 140 150
FEKAKTALAS SVRQTHQLMI NSKQLQANIE VQKIALAKAQ SDYNRRVPLG
160 170 180 190 200
NANLIGREEL QHARDAVTSA QAQLDVAIQQ YNANQAMILG TKLEDQPAVQ
210 220 230 240 250
QAATEVRNAW LALERTRIIS PMTGYVSRRA VQPGAQISPT TPLMAVVPAT
260 270 280 290 300
NMWVDANFKE TQIANMRIGQ PVTITTDIYG DDVKYTGKVV GLDMGTGSAF
310 320 330 340 350
SLLPAQNATG NWIKVVQRLP VRIELDQKQL EQYPLRIGLS TLVSVNTTNR
360 370 380 390
DGQVLANKVR STPVAVSTAR EISLAPVNKL IDDIVKANAG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | I → M in AAA23724 (PubMed:1409590).Curated | 1 | |
Sequence conflicti | 138 | K → Q in AAA23724 (PubMed:1409590).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86657 Genomic DNA Translation: AAA23724.1 U00096 Genomic DNA Translation: AAC75732.1 AP009048 Genomic DNA Translation: BAA16547.1 |
PIRi | F65048 |
RefSeqi | NP_417170.1, NC_000913.3 WP_001326681.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75732; AAC75732; b2685 BAA16547; BAA16547; BAA16547 |
GeneIDi | 947166 |
KEGGi | ecj:JW2660 eco:b2685 |
PATRICi | fig|1411691.4.peg.4054 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86657 Genomic DNA Translation: AAA23724.1 U00096 Genomic DNA Translation: AAC75732.1 AP009048 Genomic DNA Translation: BAA16547.1 |
PIRi | F65048 |
RefSeqi | NP_417170.1, NC_000913.3 WP_001326681.1, NZ_LN832404.1 |
3D structure databases
SMRi | P27303 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262946, 219 interactors 851498, 1 interactor |
ComplexPortali | CPX-4268, EmrAB-TolC multidrug efflux transport system |
DIPi | DIP-9502N |
IntActi | P27303, 4 interactors |
STRINGi | 511145.b2685 |
Protein family/group databases
TCDBi | 8.A.1.1.1, the membrane fusion protein (mfp) family |
Proteomic databases
jPOSTi | P27303 |
PaxDbi | P27303 |
PRIDEi | P27303 |
Genome annotation databases
EnsemblBacteriai | AAC75732; AAC75732; b2685 BAA16547; BAA16547; BAA16547 |
GeneIDi | 947166 |
KEGGi | ecj:JW2660 eco:b2685 |
PATRICi | fig|1411691.4.peg.4054 |
Organism-specific databases
EchoBASEi | EB1329 |
Phylogenomic databases
eggNOGi | COG1566, Bacteria |
HOGENOMi | CLU_018816_15_0_6 |
InParanoidi | P27303 |
PhylomeDBi | P27303 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11354-MONOMER MetaCyc:EG11354-MONOMER |
Miscellaneous databases
PROi | PR:P27303 |
Family and domain databases
InterProi | View protein in InterPro IPR043602, CusB_dom_1 IPR005694, Efflux_pump_mem IPR032317, HlyD_D23 |
Pfami | View protein in Pfam PF00529, HlyD, 1 hit PF16576, HlyD_D23, 1 hit |
TIGRFAMsi | TIGR00998, 8a0101, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EMRA_ECOLI | |
Accessioni | P27303Primary (citable) accession number: P27303 Secondary accession number(s): P77356 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families