UniProtKB - P27302 (TKT1_ECOLI)
Protein
Transketolase 1
Gene
tktA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Thus, catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate.2 Publications
Catalytic activityi
- D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate2 PublicationsEC:2.2.1.12 Publications
Cofactori
Protein has several cofactor binding sites:- Mg2+1 Publication, Ca2+1 Publication, Mn2+1 Publication, Co2+1 PublicationNote: Binds 1 Mg2+ ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+.1 Publication
- thiamine diphosphate1 PublicationNote: Binds 1 thiamine pyrophosphate per subunit. During the reaction, the substrate forms a covalent intermediate with the cofactor.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 26 | Substrate1 Publication | 1 | |
Sitei | 26 | Important for catalytic activity | 1 | |
Binding sitei | 66 | Thiamine pyrophosphateCombined sources2 Publications | 1 | |
Metal bindingi | 155 | Magnesium2 Publications | 1 | |
Binding sitei | 156 | Thiamine pyrophosphate; via amide nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 185 | Magnesium2 Publications | 1 | |
Binding sitei | 185 | Thiamine pyrophosphateCombined sources2 Publications | 1 | |
Metal bindingi | 187 | Magnesium; via carbonyl oxygen2 Publications | 1 | |
Binding sitei | 261 | Substrate1 Publication | 1 | |
Binding sitei | 261 | Thiamine pyrophosphateCombined sources2 Publications | 1 | |
Sitei | 261 | Important for catalytic activity | 1 | |
Binding sitei | 358 | Substrate1 Publication | 1 | |
Binding sitei | 385 | Substrate1 Publication | 1 | |
Active sitei | 411 | Proton donorCurated | 1 | |
Binding sitei | 437 | Thiamine pyrophosphate2 Publications | 1 | |
Binding sitei | 461 | Substrate1 Publication | 1 | |
Binding sitei | 469 | Substrate1 Publication | 1 | |
Binding sitei | 473 | Substrate1 Publication | 1 | |
Binding sitei | 520 | Substrate1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 114 – 116 | Thiamine pyrophosphateCombined sources2 Publications | 3 |
GO - Molecular functioni
- manganese ion binding Source: EcoCyc
- thiamine pyrophosphate binding Source: EcoCyc
- transketolase activity Source: EcoCyc
GO - Biological processi
- pentose-phosphate shunt Source: GO_Central
- pentose-phosphate shunt, non-oxidative branch Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Ligand | Calcium, Magnesium, Metal-binding, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:TRANSKETOI-MONOMER MetaCyc:TRANSKETOI-MONOMER |
BRENDAi | 2.2.1.1, 2026 |
SABIO-RKi | P27302 |
Protein family/group databases
MoonProti | P27302 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:tktA Synonyms:tkt Ordered Locus Names:b2935, JW5478 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191856 | 1 – 663 | Transketolase 1Add BLAST | 663 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P27302 |
PaxDbi | P27302 |
PRIDEi | P27302 |
PTM databases
iPTMneti | P27302 |
Interactioni
Subunit structurei
Homodimer.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 4260954, 9 interactors |
DIPi | DIP-10998N |
IntActi | P27302, 1 interactor |
STRINGi | 511145.b2935 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27302 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27302 |
Family & Domainsi
Sequence similaritiesi
Belongs to the transketolase family.Curated
Phylogenomic databases
eggNOGi | COG0021, Bacteria |
HOGENOMi | CLU_009227_0_0_6 |
InParanoidi | P27302 |
PhylomeDBi | P27302 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P27302-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSRKELANA IRALSMDAVQ KAKSGHPGAP MGMADIAEVL WRDFLKHNPQ
60 70 80 90 100
NPSWADRDRF VLSNGHGSML IYSLLHLTGY DLPMEELKNF RQLHSKTPGH
110 120 130 140 150
PEVGYTAGVE TTTGPLGQGI ANAVGMAIAE KTLAAQFNRP GHDIVDHYTY
160 170 180 190 200
AFMGDGCMME GISHEVCSLA GTLKLGKLIA FYDDNGISID GHVEGWFTDD
210 220 230 240 250
TAMRFEAYGW HVIRDIDGHD AASIKRAVEE ARAVTDKPSL LMCKTIIGFG
260 270 280 290 300
SPNKAGTHDS HGAPLGDAEI ALTREQLGWK YAPFEIPSEI YAQWDAKEAG
310 320 330 340 350
QAKESAWNEK FAAYAKAYPQ EAAEFTRRMK GEMPSDFDAK AKEFIAKLQA
360 370 380 390 400
NPAKIASRKA SQNAIEAFGP LLPEFLGGSA DLAPSNLTLW SGSKAINEDA
410 420 430 440 450
AGNYIHYGVR EFGMTAIANG ISLHGGFLPY TSTFLMFVEY ARNAVRMAAL
460 470 480 490 500
MKQRQVMVYT HDSIGLGEDG PTHQPVEQVA SLRVTPNMST WRPCDQVESA
510 520 530 540 550
VAWKYGVERQ DGPTALILSR QNLAQQERTE EQLANIARGG YVLKDCAGQP
560 570 580 590 600
ELIFIATGSE VELAVAAYEK LTAEGVKARV VSMPSTDAFD KQDAAYRESV
610 620 630 640 650
LPKAVTARVA VEAGIADYWY KYVGLNGAIV GMTTFGESAP AELLFEEFGF
660
TVDNVVAKAK ELL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 103 – 107 | VGYTA → SGVTPL in CAA48166 (PubMed:8241274).Curated | 5 | |
Sequence conflicti | 584 | P → S in CAA48166 (PubMed:8241274).Curated | 1 | |
Sequence conflicti | 584 | P → S in AAA69102 (PubMed:9278503).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68025 Genomic DNA Translation: CAA48166.1 U28377 Genomic DNA Translation: AAA69102.1 U00096 Genomic DNA Translation: AAT48155.1 AP009048 Genomic DNA Translation: BAE76998.1 M32363 Genomic DNA No translation available. |
PIRi | F65078, XJECTK |
RefSeqi | WP_000098614.1, NZ_STEB01000001.1 YP_026188.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAT48155; AAT48155; b2935 BAE76998; BAE76998; BAE76998 |
GeneIDi | 58460601 947420 |
KEGGi | ecj:JW5478 eco:b2935 |
PATRICi | fig|1411691.4.peg.3798 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68025 Genomic DNA Translation: CAA48166.1 U28377 Genomic DNA Translation: AAA69102.1 U00096 Genomic DNA Translation: AAT48155.1 AP009048 Genomic DNA Translation: BAE76998.1 M32363 Genomic DNA No translation available. |
PIRi | F65078, XJECTK |
RefSeqi | WP_000098614.1, NZ_STEB01000001.1 YP_026188.1, NC_000913.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QGD | X-ray | 1.90 | A/B | 2-663 | [»] | |
2R5N | X-ray | 1.60 | A/B | 1-663 | [»] | |
2R8O | X-ray | 1.47 | A/B | 1-663 | [»] | |
2R8P | X-ray | 1.65 | A/B | 1-663 | [»] | |
5HHT | X-ray | 1.50 | A/B | 1-663 | [»] | |
6RJC | X-ray | 1.05 | A/B | 1-663 | [»] | |
6TJ8 | X-ray | 0.92 | A/B | 1-663 | [»] | |
6TJ9 | X-ray | 0.95 | A/B | 1-663 | [»] | |
SMRi | P27302 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260954, 9 interactors |
DIPi | DIP-10998N |
IntActi | P27302, 1 interactor |
STRINGi | 511145.b2935 |
Chemistry databases
DrugBanki | DB01987, Cocarboxylase |
Protein family/group databases
MoonProti | P27302 |
PTM databases
iPTMneti | P27302 |
Proteomic databases
jPOSTi | P27302 |
PaxDbi | P27302 |
PRIDEi | P27302 |
Genome annotation databases
EnsemblBacteriai | AAT48155; AAT48155; b2935 BAE76998; BAE76998; BAE76998 |
GeneIDi | 58460601 947420 |
KEGGi | ecj:JW5478 eco:b2935 |
PATRICi | fig|1411691.4.peg.3798 |
Organism-specific databases
EchoBASEi | EB1397 |
Phylogenomic databases
eggNOGi | COG0021, Bacteria |
HOGENOMi | CLU_009227_0_0_6 |
InParanoidi | P27302 |
PhylomeDBi | P27302 |
Enzyme and pathway databases
BioCyci | EcoCyc:TRANSKETOI-MONOMER MetaCyc:TRANSKETOI-MONOMER |
BRENDAi | 2.2.1.1, 2026 |
SABIO-RKi | P27302 |
Miscellaneous databases
EvolutionaryTracei | P27302 |
PROi | PR:P27302 |
Family and domain databases
CDDi | cd02012, TPP_TK, 1 hit |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005475, Transketolase-like_Pyr-bd IPR005478, Transketolase_bac-like IPR020826, Transketolase_BS IPR033248, Transketolase_C IPR033247, Transketolase_fam IPR005474, Transketolase_N |
PANTHERi | PTHR43522, PTHR43522, 1 hit |
Pfami | View protein in Pfam PF02779, Transket_pyr, 1 hit PF02780, Transketolase_C, 1 hit PF00456, Transketolase_N, 1 hit |
SMARTi | View protein in SMART SM00861, Transket_pyr, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00232, tktlase_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00801, TRANSKETOLASE_1, 1 hit PS00802, TRANSKETOLASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TKT1_ECOLI | |
Accessioni | P27302Primary (citable) accession number: P27302 Secondary accession number(s): Q2M9Q8, Q6BF59 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 179 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families