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UniProtKB - P27300 (LPXK_ECOLI)
Protein
Tetraacyldisaccharide 4'-kinase
Gene
lpxK
Organism
Escherichia coli (strain K12)
Status
Functioni
Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).
2 PublicationsCatalytic activityi
- EC:2.7.1.1301 Publication
- ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).1 Publication EC:2.7.1.130
: lipid IV(A) biosynthesis Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine. This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 55 – 62 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- kinase activity Source: EcoCyc
- tetraacyldisaccharide 4'-kinase activity Source: EcoliWiki
GO - Biological processi
- lipid A biosynthetic process Source: EcoliWiki
- lipopolysaccharide core region biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:TETRAACYLDISACC4KIN-MONOMER |
UniPathwayi | UPA00359;UER00482 |
Names & Taxonomyi
Protein namesi | Recommended name: Tetraacyldisaccharide 4'-kinase (EC:2.7.1.1301 Publication)Alternative name(s): Lipid A 4'-kinase |
Gene namesi | Name:lpxK Synonyms:ycaH Ordered Locus Names:b0915, JW0898 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- membrane Source: EcoliWiki
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190924 | 1 – 328 | Tetraacyldisaccharide 4'-kinaseAdd BLAST | 328 |
Proteomic databases
PaxDbi | P27300 |
PRIDEi | P27300 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260014, 329 interactors |
DIPi | DIP-10126N |
IntActi | P27300, 1 interactor |
STRINGi | 511145.b0915 |
Family & Domainsi
Sequence similaritiesi
Belongs to the LpxK family.Curated
Phylogenomic databases
eggNOGi | COG1663, Bacteria |
HOGENOMi | CLU_038816_2_0_6 |
InParanoidi | P27300 |
PhylomeDBi | P27300 |
Family and domain databases
HAMAPi | MF_00409, LpxK, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003758, Tetraacyldisaccharide_4-kinase |
PANTHERi | PTHR42724, PTHR42724, 1 hit |
Pfami | View protein in Pfam PF02606, LpxK, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00682, lpxK, 1 hit |
i Sequence
Sequence statusi: Complete.
P27300-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEKIWSGES PLWRLLLPLS WLYGLVSGAI RLCYKLKLKR AWRAPVPVVV
60 70 80 90 100
VGNLTAGGNG KTPVVVWLVE QLQQRGIRVG VVSRGYGGKA ESYPLLLSAD
110 120 130 140 150
TTTAQAGDEP VLIYQRTDAP VAVSPVRSDA VKAILAQHPD VQIIVTDDGL
160 170 180 190 200
QHYRLARDVE IVVIDGVRRF GNGWWLPAGP MRERAGRLKS VDAVIVNGGV
210 220 230 240 250
PRSGEIPMHL LPGQAVNLRT GTRCDVAQLE HVVAMAGIGH PPRFFATLKM
260 270 280 290 300
CGVQPEKCVP LADHQSLNHA DVSALVSAGQ TLVMTEKDAV KCRAFAEENW
310 320
WYLPVDAQLS GDEPAKLLTQ LTLLASGN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 158 | D → N in CAA77840 (PubMed:8094880).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11796 Genomic DNA Translation: CAA77840.1 U00096 Genomic DNA Translation: AAC74001.1 AP009048 Genomic DNA Translation: BAA35661.1 |
PIRi | B64831 |
RefSeqi | NP_415435.1, NC_000913.3 WP_000570539.1, NZ_SSZK01000002.1 WP_000570544.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74001; AAC74001; b0915 BAA35661; BAA35661; BAA35661 |
GeneIDi | 945526 |
KEGGi | ecj:JW0898 eco:b0915 |
PATRICi | fig|511145.12.peg.946 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11796 Genomic DNA Translation: CAA77840.1 U00096 Genomic DNA Translation: AAC74001.1 AP009048 Genomic DNA Translation: BAA35661.1 |
PIRi | B64831 |
RefSeqi | NP_415435.1, NC_000913.3 WP_000570539.1, NZ_SSZK01000002.1 WP_000570544.1, NZ_LN832404.1 |
3D structure databases
SMRi | P27300 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260014, 329 interactors |
DIPi | DIP-10126N |
IntActi | P27300, 1 interactor |
STRINGi | 511145.b0915 |
Proteomic databases
PaxDbi | P27300 |
PRIDEi | P27300 |
Genome annotation databases
EnsemblBacteriai | AAC74001; AAC74001; b0915 BAA35661; BAA35661; BAA35661 |
GeneIDi | 945526 |
KEGGi | ecj:JW0898 eco:b0915 |
PATRICi | fig|511145.12.peg.946 |
Organism-specific databases
EchoBASEi | EB1381 |
Phylogenomic databases
eggNOGi | COG1663, Bacteria |
HOGENOMi | CLU_038816_2_0_6 |
InParanoidi | P27300 |
PhylomeDBi | P27300 |
Enzyme and pathway databases
UniPathwayi | UPA00359;UER00482 |
BioCyci | EcoCyc:TETRAACYLDISACC4KIN-MONOMER |
Miscellaneous databases
PROi | PR:P27300 |
Family and domain databases
HAMAPi | MF_00409, LpxK, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003758, Tetraacyldisaccharide_4-kinase |
PANTHERi | PTHR42724, PTHR42724, 1 hit |
Pfami | View protein in Pfam PF02606, LpxK, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00682, lpxK, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LPXK_ECOLI | |
Accessioni | P27300Primary (citable) accession number: P27300 Secondary accession number(s): P75842, Q9R7Q6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 23, 2022 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families