Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GTPase ArgK

Gene

argK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and hydrolyzes GTP (PubMed:18950999). Likely functions as a G-protein chaperone that assists AdoCbl cofactor delivery to the methylmalonyl-CoA mutase (MCM) ScpA and reactivation of the enzyme during catalysis.Curated1 Publication

Miscellaneous

Part of an operon that encodes enzymes converting succinate to propionate.1 Publication

Caution

Was originally thought to be a membrane protein kinase involved in phosphorylation of the AO and LAO periplasmic-binding proteins (PubMed:9733684). However, its role needs to be reexamined.1 Publication

Catalytic activityi

GTP + H2O = GDP + phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi61 – 68ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • GTPase activity Source: EcoCyc
  • GTP binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11445-MONOMER
MetaCyc:EG11445-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase ArgK1 Publication (EC:3.6.5.-1 Publication)
Alternative name(s):
G-protein chaperoneCurated
Gene namesi
Name:argK
Synonyms:ygfD
Ordered Locus Names:b2918, JW2885
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11445 argK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001578161 – 331GTPase ArgKAdd BLAST331

Proteomic databases

PaxDbiP27254
PRIDEiP27254

Interactioni

Subunit structurei

Monomer. Interacts with the methylmalonyl-CoA mutase ScpA.1 Publication

Protein-protein interaction databases

BioGridi4259325, 18 interactors
IntActiP27254, 13 interactors
STRINGi316385.ECDH10B_3093

Structurei

Secondary structure

1331
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP27254
SMRiP27254
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27254

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DJT Bacteria
COG1703 LUCA
HOGENOMiHOG000003902
InParanoidiP27254
KOiK07588
PhylomeDBiP27254

Family and domain databases

InterProiView protein in InterPro
IPR005129 GTPase_ArgK
IPR027417 P-loop_NTPase
PANTHERiPTHR23408:SF3 PTHR23408:SF3, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00750 lao, 1 hit

Sequencei

Sequence statusi: Complete.

P27254-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MINEATLAES IRRLRQGERA TLAQAMTLVE SRHPRHQALS TQLLDAIMPY
60 70 80 90 100
CGNTLRLGVT GTPGAGKSTF LEAFGMLLIR EGLKVAVIAV DPSSPVTGGS
110 120 130 140 150
ILGDKTRMND LARAEAAFIR PVPSSGHLGG ASQRARELML LCEAAGYDVV
160 170 180 190 200
IVETVGVGQS ETEVARMVDC FISLQIAGGG DDLQGIKKGL MEVADLIVIN
210 220 230 240 250
KDDGDNHTNV AIARHMYESA LHILRRKYDE WQPRVLTCSA LEKRGIDEIW
260 270 280 290 300
HAIIDFKTAL TASGRLQQVR QQQSVEWLRK QTEEEVLNHL FANEDFDRYY
310 320 330
RQTLLAVKNN TLSPRTGLRQ LSEFIQTQYF D
Length:331
Mass (Da):36,704
Last modified:October 1, 1996 - v2
Checksum:i02C27AD2000C6137
GO

Sequence cautioni

The sequence CAA47312 differs from that shown. Reason: Frameshift at several positions.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti68S → R in strain: RC101. 1
Natural varianti154T → A in strain: RC101. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA Translation: CAA47312.1 Frameshift.
U28377 Genomic DNA Translation: AAA69085.1
U00096 Genomic DNA Translation: AAC75955.1
AP009048 Genomic DNA Translation: BAE76982.1
PIRiE65076
RefSeqiNP_417393.1, NC_000913.3
WP_000606525.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75955; AAC75955; b2918
BAE76982; BAE76982; BAE76982
GeneIDi947412
KEGGiecj:JW2885
eco:b2918
PATRICifig|1411691.4.peg.3814

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66836 Genomic DNA Translation: CAA47312.1 Frameshift.
U28377 Genomic DNA Translation: AAA69085.1
U00096 Genomic DNA Translation: AAC75955.1
AP009048 Genomic DNA Translation: BAE76982.1
PIRiE65076
RefSeqiNP_417393.1, NC_000913.3
WP_000606525.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P67X-ray1.80A2-331[»]
ProteinModelPortaliP27254
SMRiP27254
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259325, 18 interactors
IntActiP27254, 13 interactors
STRINGi316385.ECDH10B_3093

Proteomic databases

PaxDbiP27254
PRIDEiP27254

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75955; AAC75955; b2918
BAE76982; BAE76982; BAE76982
GeneIDi947412
KEGGiecj:JW2885
eco:b2918
PATRICifig|1411691.4.peg.3814

Organism-specific databases

EchoBASEiEB1415
EcoGeneiEG11445 argK

Phylogenomic databases

eggNOGiENOG4105DJT Bacteria
COG1703 LUCA
HOGENOMiHOG000003902
InParanoidiP27254
KOiK07588
PhylomeDBiP27254

Enzyme and pathway databases

BioCyciEcoCyc:EG11445-MONOMER
MetaCyc:EG11445-MONOMER

Miscellaneous databases

EvolutionaryTraceiP27254
PROiPR:P27254

Family and domain databases

InterProiView protein in InterPro
IPR005129 GTPase_ArgK
IPR027417 P-loop_NTPase
PANTHERiPTHR23408:SF3 PTHR23408:SF3, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00750 lao, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiARGK_ECOLI
AccessioniPrimary (citable) accession number: P27254
Secondary accession number(s): Q2M9S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again