UniProtKB - P27253 (SCPA_ECOLI)
Protein
Methylmalonyl-CoA mutase
Gene
scpA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA. Could be part of a pathway that converts succinate to propionate.2 Publications
Catalytic activityi
- EC:5.4.99.22 Publications
Cofactori
adenosylcob(III)alamin2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 597 | Cobalt (cobalamin axial ligand)By similarity | 1 |
GO - Molecular functioni
- cobalamin binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- methylmalonyl-CoA mutase activity Source: EcoCyc
Keywordsi
Molecular function | Isomerase |
Ligand | Cobalamin, Cobalt, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:METHYLMALONYL-COA-MUT-MONOMER MetaCyc:METHYLMALONYL-COA-MUT-MONOMER |
Chemistry databases
SwissLipidsi | SLP:000001257 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:scpA Synonyms:sbm, yliK Ordered Locus Names:b2917, JW2884 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194277 | 1 – 714 | Methylmalonyl-CoA mutaseAdd BLAST | 714 |
Proteomic databases
PaxDbi | P27253 |
PRIDEi | P27253 |
Interactioni
Subunit structurei
Homodimer.
Interacts with ArgK.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262330, 27 interactors |
IntActi | P27253, 6 interactors |
STRINGi | 511145.b2917 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 584 – 714 | B12-bindingPROSITE-ProRule annotationAdd BLAST | 131 |
Sequence similaritiesi
Belongs to the methylmalonyl-CoA mutase family.Curated
Phylogenomic databases
eggNOGi | COG1884, Bacteria COG2185, Bacteria |
HOGENOMi | CLU_009523_3_1_6 |
InParanoidi | P27253 |
PhylomeDBi | P27253 |
Family and domain databases
InterProi | View protein in InterPro IPR006159, Acid_CoA_mut_C IPR016176, Cbl-dep_enz_cat IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006099, MeMalonylCoA_mutase_a/b_cat IPR006098, MMCoA_mutase_a_cat |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit PF01642, MM_CoA_mutase, 1 hit |
SUPFAMi | SSF51703, SSF51703, 1 hit SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR00640, acid_CoA_mut_C, 1 hit TIGR00641, acid_CoA_mut_N, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit PS00544, METMALONYL_COA_MUTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P27253-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNVQEWQQL ANKELSRREK TVDSLVHQTA EGIAIKPLYT EADLDNLEVT
60 70 80 90 100
GTLPGLPPYV RGPRATMYTA QPWTIRQYAG FSTAKESNAF YRRNLAAGQK
110 120 130 140 150
GLSVAFDLAT HRGYDSDNPR VAGDVGKAGV AIDTVEDMKV LFDQIPLDKM
160 170 180 190 200
SVSMTMNGAV LPVLAFYIVA AEEQGVTPDK LTGTIQNDIL KEYLCRNTYI
210 220 230 240 250
YPPKPSMRII ADIIAWCSGN MPRFNTISIS GYHMGEAGAN CVQQVAFTLA
260 270 280 290 300
DGIEYIKAAI SAGLKIDDFA PRLSFFFGIG MDLFMNVAML RAARYLWSEA
310 320 330 340 350
VSGFGAQDPK SLALRTHCQT SGWSLTEQDP YNNVIRTTIE ALAATLGGTQ
360 370 380 390 400
SLHTNAFDEA LGLPTDFSAR IARNTQIIIQ EESELCRTVD PLAGSYYIES
410 420 430 440 450
LTDQIVKQAR AIIQQIDEAG GMAKAIEAGL PKRMIEEASA REQSLIDQGK
460 470 480 490 500
RVIVGVNKYK LDHEDETDVL EIDNVMVRNE QIASLERIRA TRDDAAVTAA
510 520 530 540 550
LNALTHAAQH NENLLAAAVN AARVRATLGE ISDALEVAFD RYLVPSQCVT
560 570 580 590 600
GVIAQSYHQS EKSASEFDAI VAQTEQFLAD NGRRPRILIA KMGQDGHDRG
610 620 630 640 650
AKVIASAYSD LGFDVDLSPM FSTPEEIARL AVENDVHVVG ASSLAAGHKT
660 670 680 690 700
LIPELVEALK KWGREDICVV AGGVIPPQDY AFLQERGVAA IYGPGTPMLD
710
SVRDVLNLIS QHHD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 356 | A → V in CAA47311 (PubMed:1355087).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66836 Genomic DNA Translation: CAA47311.1 U28377 Genomic DNA Translation: AAA69084.1 U00096 Genomic DNA Translation: AAC75954.1 AP009048 Genomic DNA Translation: BAE76981.1 |
PIRi | D65076 |
RefSeqi | NP_417392.1, NC_000913.3 WP_000073223.1, NZ_SSZK01000003.1 WP_000073228.1, NZ_CP047127.1 |
Genome annotation databases
EnsemblBacteriai | AAC75954; AAC75954; b2917 BAE76981; BAE76981; BAE76981 |
GeneIDi | 945576 |
KEGGi | ecj:JW2884 eco:b2917 |
PATRICi | fig|1411691.4.peg.3815 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66836 Genomic DNA Translation: CAA47311.1 U28377 Genomic DNA Translation: AAA69084.1 U00096 Genomic DNA Translation: AAC75954.1 AP009048 Genomic DNA Translation: BAE76981.1 |
PIRi | D65076 |
RefSeqi | NP_417392.1, NC_000913.3 WP_000073223.1, NZ_SSZK01000003.1 WP_000073228.1, NZ_CP047127.1 |
3D structure databases
SMRi | P27253 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262330, 27 interactors |
IntActi | P27253, 6 interactors |
STRINGi | 511145.b2917 |
Chemistry databases
SwissLipidsi | SLP:000001257 |
Proteomic databases
PaxDbi | P27253 |
PRIDEi | P27253 |
Genome annotation databases
EnsemblBacteriai | AAC75954; AAC75954; b2917 BAE76981; BAE76981; BAE76981 |
GeneIDi | 945576 |
KEGGi | ecj:JW2884 eco:b2917 |
PATRICi | fig|1411691.4.peg.3815 |
Organism-specific databases
EchoBASEi | EB1414 |
Phylogenomic databases
eggNOGi | COG1884, Bacteria COG2185, Bacteria |
HOGENOMi | CLU_009523_3_1_6 |
InParanoidi | P27253 |
PhylomeDBi | P27253 |
Enzyme and pathway databases
BioCyci | EcoCyc:METHYLMALONYL-COA-MUT-MONOMER MetaCyc:METHYLMALONYL-COA-MUT-MONOMER |
Miscellaneous databases
PROi | PR:P27253 |
Family and domain databases
InterProi | View protein in InterPro IPR006159, Acid_CoA_mut_C IPR016176, Cbl-dep_enz_cat IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006099, MeMalonylCoA_mutase_a/b_cat IPR006098, MMCoA_mutase_a_cat |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit PF01642, MM_CoA_mutase, 1 hit |
SUPFAMi | SSF51703, SSF51703, 1 hit SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR00640, acid_CoA_mut_C, 1 hit TIGR00641, acid_CoA_mut_N, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit PS00544, METMALONYL_COA_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SCPA_ECOLI | |
Accessioni | P27253Primary (citable) accession number: P27253 Secondary accession number(s): Q2M9S5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 29, 2003 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families