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Entry version 154 (02 Dec 2020)
Sequence version 2 (01 Feb 1995)
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Protein

Aldehyde reductase Ahr

Gene

ahr

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reduction of a wide range of aldehydes including aliphatic fatty aldehydes (C4-C16), into their corresponding alcohols. Has a strong preference for NADPH over NADH as the electron donor. Cannot use glyceraldehyde or a ketone as substrate. Is a relevant source of NADPH-dependent aldehyde reductase activity in E.coli. The in vivo functions of Ahr has yet to be determined.3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 333 sec(-1) for acetaldehyde reduction. kcat is 207 sec(-1) for propionaldehyde reduction. kcat is 464 sec(-1) for butyraldehyde reduction. kcat is 51 sec(-1) for isobutyraldehyde reduction. kcat is 78 sec(-1) for crotonaldehyde reduction. kcat is 312 sec(-1) for glutaraldehyde reduction. kcat is 2.6 sec(-1) for 5-hydroxyvalerate reduction. kcat is 419 sec(-1) for hexanaldehyde reduction. kcat is 313 sec(-1) for benzaldehyde reduction. kcat is 224 sec(-1) for furfural reduction. kcat is 13.8 sec(-1) for butanol oxidation. kcat is 12.9 sec(-1) for 1,4-butanediol oxidation.
  1. KM=73.4 mM for acetaldehyde1 Publication
  2. KM=19.6 mM for propionaldehyde1 Publication
  3. KM=2.1 mM for butyraldehyde1 Publication
  4. KM=193.7 mM for isobutyraldehyde1 Publication
  5. KM=2.4 mM for crotonaldehyde1 Publication
  6. KM=5.8 mM for glutaraldehyde1 Publication
  7. KM=59.7 mM for 5-hydroxyvalerate1 Publication
  8. KM=0.34 mM for hexanaldehyde1 Publication
  9. KM=0.24 mM for benzaldehyde1 Publication
  10. KM=0.22 mM for furfural1 Publication
  11. KM=3.5 mM for butanol1 Publication
  12. KM=24.1 mM for 1,4-butanediol1 Publication
  13. KM=0.06 mM for NADPH1 Publication
  14. KM=0.076 mM for NADP+1 Publication

    Temperature dependencei

    Optimum temperature is 37-50 degrees Celsius using butyraldehyde as substrate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi38Zinc 1; catalyticBy similarity1
    Metal bindingi63Zinc 1; catalyticBy similarity1
    Metal bindingi96Zinc 2By similarity1
    Metal bindingi99Zinc 2By similarity1
    Metal bindingi102Zinc 2By similarity1
    Metal bindingi110Zinc 2By similarity1
    Metal bindingi152Zinc 1; catalyticBy similarity1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    Biological processFatty acid metabolism, Lipid metabolism
    LigandMetal-binding, NADP, Zinc

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:EG11436-MONOMER
    MetaCyc:EG11436-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Aldehyde reductase Ahr (EC:1.1.1.2)
    Alternative name(s):
    Zinc-dependent alcohol dehydrogenase Ahr
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:ahr
    Synonyms:yjgB
    Ordered Locus Names:b4269, JW5761
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

    This enzyme can be applied to the microbial production of fatty alcohols that have numerous applications as fuels, fragrances, emollients, food additives, and detergents. Thus, together with complementing enzymes, the broad substrate specificity of Ahr opens the road for direct and tailored enzyme-catalyzed conversion of lipids into user-ready chemical commodities.1 Publication

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001608941 – 339Aldehyde reductase AhrAdd BLAST339

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    P27250

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P27250

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P27250

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...
    BioGRIDi
    4263049, 10 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    511145.b4269

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1339
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P27250

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG1064, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_026673_20_2_6

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P27250

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P27250

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR013149, ADH_C
    IPR013154, ADH_N
    IPR002328, ADH_Zn_CS
    IPR029752, D-isomer_DH_CS1
    IPR011032, GroES-like_sf
    IPR036291, NAD(P)-bd_dom_sf
    IPR020843, PKS_ER

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF08240, ADH_N, 1 hit
    PF00107, ADH_zinc_N, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00829, PKS_ER, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF50129, SSF50129, 1 hit
    SSF51735, SSF51735, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00059, ADH_ZINC, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P27250-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSMIKSYAAK EAGGELEVYE YDPGELRPQD VEVQVDYCGI CHSDLSMIDN
    60 70 80 90 100
    EWGFSQYPLV AGHEVIGRVV ALGSAAQDKG LQVGQRVGIG WTARSCGHCD
    110 120 130 140 150
    ACISGNQINC EQGAVPTIMN RGGFAEKLRA DWQWVIPLPE NIDIESAGPL
    160 170 180 190 200
    LCGGITVFKP LLMHHITATS RVGVIGIGGL GHIAIKLLHA MGCEVTAFSS
    210 220 230 240 250
    NPAKEQEVLA MGADKVVNSR DPQALKALAG QFDLIINTVN VSLDWQPYFE
    260 270 280 290 300
    ALTYGGNFHT VGAVLTPLSV PAFTLIAGDR SVSGSATGTP YELRKLMRFA
    310 320 330
    ARSKVAPTTE LFPMSKINDA IQHVRDGKAR YRVVLKADF
    Length:339
    Mass (Da):36,502
    Last modified:February 1, 1995 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0854DDEFA16B9EEE
    GO

    <p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence AAA97166 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti331 – 339YRVVLKADF → TAWC in AAA72122 (Ref. 1) Curated9

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    M96355 Genomic DNA Translation: AAA72122.1
    U14003 Genomic DNA Translation: AAA97166.1 Different initiation.
    U00096 Genomic DNA Translation: AAC77226.2
    AP009048 Genomic DNA Translation: BAE78266.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S56495

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_418690.4, NC_000913.3
    WP_001309160.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC77226; AAC77226; b4269
    BAE78266; BAE78266; BAE78266

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    948802

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW5761
    eco:b4269

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|511145.12.peg.4400

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M96355 Genomic DNA Translation: AAA72122.1
    U14003 Genomic DNA Translation: AAA97166.1 Different initiation.
    U00096 Genomic DNA Translation: AAC77226.2
    AP009048 Genomic DNA Translation: BAE78266.1
    PIRiS56495
    RefSeqiNP_418690.4, NC_000913.3
    WP_001309160.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    7BU2X-ray1.55A/B1-339[»]
    SMRiP27250
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    BioGRIDi4263049, 10 interactors
    STRINGi511145.b4269

    Proteomic databases

    jPOSTiP27250
    PaxDbiP27250
    PRIDEiP27250

    Genome annotation databases

    EnsemblBacteriaiAAC77226; AAC77226; b4269
    BAE78266; BAE78266; BAE78266
    GeneIDi948802
    KEGGiecj:JW5761
    eco:b4269
    PATRICifig|511145.12.peg.4400

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB1406

    Phylogenomic databases

    eggNOGiCOG1064, Bacteria
    HOGENOMiCLU_026673_20_2_6
    InParanoidiP27250
    PhylomeDBiP27250

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11436-MONOMER
    MetaCyc:EG11436-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P27250

    Family and domain databases

    InterProiView protein in InterPro
    IPR013149, ADH_C
    IPR013154, ADH_N
    IPR002328, ADH_Zn_CS
    IPR029752, D-isomer_DH_CS1
    IPR011032, GroES-like_sf
    IPR036291, NAD(P)-bd_dom_sf
    IPR020843, PKS_ER
    PfamiView protein in Pfam
    PF08240, ADH_N, 1 hit
    PF00107, ADH_zinc_N, 1 hit
    SMARTiView protein in SMART
    SM00829, PKS_ER, 1 hit
    SUPFAMiSSF50129, SSF50129, 1 hit
    SSF51735, SSF51735, 1 hit
    PROSITEiView protein in PROSITE
    PS00059, ADH_ZINC, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAHR_ECOLI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P27250
    Secondary accession number(s): P76812, Q2M640
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: February 1, 1995
    Last modified: December 2, 2020
    This is version 154 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
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