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UniProtKB - P27243 (RFAL_ECOLI)
Protein
O-antigen ligase
Gene
rfaL
Organism
Escherichia coli (strain K12)
Status
Functioni
Adds the O-antigen on the glucose group of LPS.
: LPS core biosynthesis Pathwayi
This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- glycosyltransferase activity Source: EcoCyc
- ligase activity Source: UniProtKB-KW
GO - Biological processi
- lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Biological process | Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11424-MONOMER |
UniPathwayi | UPA00958 |
Protein family/group databases
TCDBi | 9.B.67.5.1, the o-antigen polymerase (oap) family |
Names & Taxonomyi
Protein namesi | Recommended name: O-antigen ligase |
Gene namesi | Name:rfaL Synonyms:waaL Ordered Locus Names:b3622, JW3597 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 17 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 18 – 38 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 39 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 40 – 60 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 61 – 71 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 72 – 92 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 93 – 105 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 106 – 126 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 127 – 135 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 136 – 156 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 157 – 168 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 169 – 189 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 190 – 193 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 194 – 214 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 215 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 216 – 236 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 237 – 241 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 242 – 262 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 263 – 346 | PeriplasmicSequence analysisAdd BLAST | 84 | |
Transmembranei | 347 – 367 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 368 – 369 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 370 – 390 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 391 – 411 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 412 – 419 | Cytoplasmic1 Publication | 8 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097284 | 1 – 419 | O-antigen ligaseAdd BLAST | 419 |
Proteomic databases
PaxDbi | P27243 |
PRIDEi | P27243 |
Expressioni
Inductioni
Expressed in both exponetial and stationary phase in rich medium (at protein level).1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263298, 155 interactors |
DIPi | DIP-10673N |
IntActi | P27243, 1 interactor |
STRINGi | 511145.b3622 |
Structurei
Family & Domainsi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3307, Bacteria |
HOGENOMi | CLU_054167_0_0_6 |
Family and domain databases
InterProi | View protein in InterPro IPR007016, O-antigen_ligase-related |
Pfami | View protein in Pfam PF04932, Wzy_C, 1 hit |
i Sequence
Sequence statusi: Complete.
P27243-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLTSFKLHSL KPYTLKSSMI LEIITYILCF FSMIIAFVDN TFSIKIYNIT
60 70 80 90 100
AIVCLLSLIL RGRQENYNIK NLILPLSIFL IGLLDLIWYS AFKVDNSPFR
110 120 130 140 150
ATYHSYLNTA KIFIFGSFIV FLTLTSQLKS KKESVLYTLY SLSFLIAGYA
160 170 180 190 200
MYINSIHEND RISFGVGTAT GAAYSTMLIG IVSGVAILYT KKNHPFLFLL
210 220 230 240 250
NSCAVLYVLA LTQTRATLLL FPIICVAALI AYYNKSPKKF TSSIVLLIAI
260 270 280 290 300
LASIVIIFNK PIQNRYNEAL NDLNSYTNAN SVTSLGARLA MYEIGLNIFI
310 320 330 340 350
KSPFSFRSAE SRAESMNLLV AEHNRLRGAL EFSNVHLHNE IIEAGSLKGL
360 370 380 390 400
MGIFSTLFLY FSLFYIAYKK RALGLLILTL GIVGIGLSDV IIWARSIPII
410
IISAIVLLLV INNRNNTIN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 205 – 224 | VLYVL…LFPII → GTLCSGANTNQSNPTPVPYN in AAA24524 (PubMed:1624462).CuratedAdd BLAST | 20 | |
Sequence conflicti | 307 | R → T in AAA24524 (PubMed:1624462).Curated | 1 | |
Sequence conflicti | 344 | A → R in AAA24524 (PubMed:1624462).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95398 Genomic DNA Translation: AAA24524.1 U00039 Genomic DNA Translation: AAB18599.1 U00096 Genomic DNA Translation: AAC76646.1 AP009048 Genomic DNA Translation: BAE77670.1 |
PIRi | S47843 |
RefSeqi | NP_418079.1, NC_000913.3 WP_001395405.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76646; AAC76646; b3622 BAE77670; BAE77670; BAE77670 |
GeneIDi | 948148 |
KEGGi | ecj:JW3597 eco:b3622 |
PATRICi | fig|1411691.4.peg.3084 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M95398 Genomic DNA Translation: AAA24524.1 U00039 Genomic DNA Translation: AAB18599.1 U00096 Genomic DNA Translation: AAC76646.1 AP009048 Genomic DNA Translation: BAE77670.1 |
PIRi | S47843 |
RefSeqi | NP_418079.1, NC_000913.3 WP_001395405.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4263298, 155 interactors |
DIPi | DIP-10673N |
IntActi | P27243, 1 interactor |
STRINGi | 511145.b3622 |
Protein family/group databases
TCDBi | 9.B.67.5.1, the o-antigen polymerase (oap) family |
Proteomic databases
PaxDbi | P27243 |
PRIDEi | P27243 |
Genome annotation databases
EnsemblBacteriai | AAC76646; AAC76646; b3622 BAE77670; BAE77670; BAE77670 |
GeneIDi | 948148 |
KEGGi | ecj:JW3597 eco:b3622 |
PATRICi | fig|1411691.4.peg.3084 |
Organism-specific databases
EchoBASEi | EB1394 |
Phylogenomic databases
eggNOGi | COG3307, Bacteria |
HOGENOMi | CLU_054167_0_0_6 |
Enzyme and pathway databases
UniPathwayi | UPA00958 |
BioCyci | EcoCyc:EG11424-MONOMER |
Miscellaneous databases
PHI-basei | PHI:3106 |
PROi | PR:P27243 |
Family and domain databases
InterProi | View protein in InterPro IPR007016, O-antigen_ligase-related |
Pfami | View protein in Pfam PF04932, Wzy_C, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RFAL_ECOLI | |
Accessioni | P27243Primary (citable) accession number: P27243 Secondary accession number(s): Q2M7T6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 29, 2003 | |
Last modified: | February 23, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways