UniProtKB - P27232 (VL1_HPV35)
Protein
Major capsid protein L1
Gene
L1
Organism
Human papillomavirus type 35
Status
Functioni
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.UniRule annotation
GO - Molecular functioni
- structural molecule activity Source: UniProtKB-UniRule
GO - Biological processi
- endocytosis involved in viral entry into host cell Source: UniProtKB-KW
- virion attachment to host cell Source: UniProtKB-UniRule
Keywordsi
Biological process | Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: Major capsid protein L1UniRule annotation |
Gene namesi | Name:L1UniRule annotation |
Organismi | Human papillomavirus type 35 |
Taxonomic identifieri | 10587 [NCBI] |
Taxonomic lineagei | Viruses › Monodnaviria › Shotokuvirae › Cossaviricota › Papovaviricetes › Zurhausenvirales › Papillomaviridae › Firstpapillomavirinae › Alphapapillomavirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host nucleus UniRule annotation
GO - Cellular componenti
- host cell nucleus Source: UniProtKB-SubCell
- T=7 icosahedral viral capsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Capsid protein, Host nucleus, T=7 icosahedral capsid protein, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000133519 | 1 – 502 | Major capsid protein L1Add BLAST | 502 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 175 | Interchain (with C-426)UniRule annotation | ||
Disulfide bondi | 426 | Interchain (with C-175)UniRule annotation |
Keywords - PTMi
Disulfide bondInteractioni
Subunit structurei
Self-assembles into homopentamers. The capsid has an icosahedral symmetry and consists of 72 capsomers, with each capsomer being a pentamer of L1.
Interacts with the minor capsid protein L2; this interaction is necessary for viral genome encapsidation.
Interacts with protein E2; this interaction enhances E2-dependent replication and transcription activation.
UniRule annotationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27232 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27232 |
Family & Domainsi
Sequence similaritiesi
Belongs to the papillomaviridae L1 protein family.UniRule annotation
Family and domain databases
Gene3Di | 2.60.175.20, 2 hits |
HAMAPi | MF_04002, PPV_L1, 1 hit |
InterProi | View protein in InterPro IPR002210, Capsid_L1_Papillomavir IPR036973, Capsid_L1_sf_Papillomavir IPR011222, dsDNA_vir_gr_I_capsid |
Pfami | View protein in Pfam PF00500, Late_protein_L1, 1 hit |
PRINTSi | PR00865, HPVCAPSIDL1 |
SUPFAMi | SSF88648, SSF88648, 1 hit |
i Sequence
Sequence statusi: Complete.
P27232-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLWRSNEAT VYLPPVSVSK VVSTDEYVTR TNIYYHAGSS RLLAVGHPYY
60 70 80 90 100
AIKKQDSNKI AVPKVSGLQY RVFRVKLPDP NKFGFPDTSF YDPASQRLVW
110 120 130 140 150
ACTGVEVGRG QPLGVGISGH PLLNKLDDTE NSNKYVGNSG TDNRECISMD
160 170 180 190 200
YKQTQLCLIG CRPPIGEHWG KGTPCNANQV KAGECPPLEL LNTVLQDGDM
210 220 230 240 250
VDTGFGAMDF TTLQANKSDV PLDICSSICK YPDYLKMVSE PYGDMLFFYL
260 270 280 290 300
RREQMFVRHL FNRAGTVGET VPADLYIKGT TGTLPSTSYF PTPSGSMVTS
310 320 330 340 350
DAQIFNKPYW LQRAQGHNNG ICWSNQLFVT VVDTTRSTNM SVCSAVSSSD
360 370 380 390 400
STYKNDNFKE YLRHGEEYDL QFIFQLCKIT LTADVMTYIH SMNPSILEDW
410 420 430 440 450
NFGLTPPPSG TLEDTYRYVT SQAVTCQKPS APKPKDDPLK NYTFWEVDLK
460 470 480 490 500
EKFSADLDQF PLGRKFLLQA GLKARPNFRL GKRAAPASTS KKSSTKRRKV
KS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 – 95 | AS → CL in AAA46972 (PubMed:1310198).Curated | 2 | |
Sequence conflicti | 132 | S → L in AAA46972 (PubMed:1310198).Curated | 1 | |
Sequence conflicti | 140 | G → GNSG (PubMed:1310198).Curated | 1 | |
Sequence conflicti | 482 | K → R in AAA46972 (PubMed:1310198).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74477 Genomic DNA Translation: CAA52566.1 M74117 Genomic DNA Translation: AAA46972.1 S40240 Genomic DNA Translation: AAB22564.1 |
PIRi | G40824, P1WL35 S36526 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X74477 Genomic DNA Translation: CAA52566.1 M74117 Genomic DNA Translation: AAA46972.1 S40240 Genomic DNA Translation: AAB22564.1 |
PIRi | G40824, P1WL35 S36526 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2R5J | X-ray | 3.30 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O | 21-401 | [»] | |
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O | 437-472 | [»] | ||||
SMRi | P27232 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27232 |
Family and domain databases
Gene3Di | 2.60.175.20, 2 hits |
HAMAPi | MF_04002, PPV_L1, 1 hit |
InterProi | View protein in InterPro IPR002210, Capsid_L1_Papillomavir IPR036973, Capsid_L1_sf_Papillomavir IPR011222, dsDNA_vir_gr_I_capsid |
Pfami | View protein in Pfam PF00500, Late_protein_L1, 1 hit |
PRINTSi | PR00865, HPVCAPSIDL1 |
SUPFAMi | SSF88648, SSF88648, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VL1_HPV35 | |
Accessioni | P27232Primary (citable) accession number: P27232 Secondary accession number(s): Q90077 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families