UniProtKB - P27216 (ANX13_HUMAN)
Annexin A13
ANXA13
Functioni
Binds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner (PubMed:27676605, PubMed:30610115).
Half-maximal membrane binding requires about 60 µM calcium. Does not bind to membranes that lack phospholipids with an acidic headgroup (PubMed:27676605).
2 PublicationsBinds to membranes enriched in phosphatidylserine or phosphatidylglycerol in a calcium-dependent manner, but requires higher calcium levels for membrane binding than isoform A. Half-maximal membrane binding requires about 320 µM calcium.
1 PublicationGO - Molecular functioni
- calcium-dependent phospholipid binding Source: UniProtKB
- calcium ion binding Source: UniProtKB
- phosphatidylglycerol binding Source: UniProtKB
- phosphatidylserine binding Source: UniProtKB
GO - Biological processi
- cell differentiation Source: UniProtKB
- negative regulation of Golgi to plasma membrane protein transport Source: CAFA
- positive regulation of Golgi to plasma membrane protein transport Source: CAFA
Keywordsi
Ligand | Calcium, Calcium/phospholipid-binding |
Enzyme and pathway databases
PathwayCommonsi | P27216 |
SignaLinki | P27216 |
Names & Taxonomyi
Protein namesi | Recommended name: Annexin A13Alternative name(s): Annexin XIII Annexin-13 Intestine-specific annexin1 Publication Short name: ISA1 Publication |
Gene namesi | Name:ANXA13 Synonyms:ANX13 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:536, ANXA13 |
MIMi | 602573, gene |
neXtProti | NX_P27216 |
VEuPathDBi | HostDB:ENSG00000104537 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 1 Publication
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Other locations
- Cytoplasmic vesicle By similarity
Note: Myristoylation anchors the protein to the membrane, but the protein displays also calcium-dependent, reversible binding to lipid membranes (PubMed:30610115). Associated with the plasma membrane of undifferentiated, proliferating crypt epithelial cells as well as differentiated villus enterocytes (PubMed:1530946).2 Publications
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular space Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- apical plasma membrane Source: CAFA
- basolateral plasma membrane Source: CAFA
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic vesicle Source: UniProtKB-KW
- exocytic vesicle Source: CAFA
- extrinsic component of membrane Source: UniProtKB
- membrane raft Source: CAFA
Keywords - Cellular componenti
Cell membrane, Cytoplasmic vesicle, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Loss of myristoylation. Loss of location at the cell membrane. 1 Publication | 1 | |
Mutagenesisi | 32 – 34 | TNE → PGP: Loss of dimerization. No effect on calcium-dependent membrane binding and location at the cell membrane. 1 Publication | 3 | |
Mutagenesisi | 73 | S → C: Slightly increased dimerization. Decreases calcium-dependent membrane binding and location at the cell membrane. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 312 |
OpenTargetsi | ENSG00000104537 |
PharmGKBi | PA24826 |
Miscellaneous databases
Pharosi | P27216, Tbio |
Genetic variation databases
BioMutai | ANXA13 |
DMDMi | 281185504 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000067514 | 2 – 316 | Annexin A13Add BLAST | 315 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine2 Publications | 1 |
Keywords - PTMi
Lipoprotein, MyristateProteomic databases
EPDi | P27216 |
jPOSTi | P27216 |
MassIVEi | P27216 |
MaxQBi | P27216 |
PaxDbi | P27216 |
PeptideAtlasi | P27216 |
PRIDEi | P27216 |
ProteomicsDBi | 54377 [P27216-1] 54378 [P27216-2] |
PTM databases
GlyGeni | P27216, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P27216 |
PhosphoSitePlusi | P27216 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000104537, Expressed in intestine and 106 other tissues |
ExpressionAtlasi | P27216, baseline and differential |
Genevisiblei | P27216, HS |
Organism-specific databases
HPAi | ENSG00000104537, Tissue enhanced (gallbladder, intestine) |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 106809, 19 interactors |
IntActi | P27216, 6 interactors |
STRINGi | 9606.ENSP00000262219 |
Miscellaneous databases
RNActi | P27216, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P27216 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 14 – 85 | Annexin 1PROSITE-ProRule annotationAdd BLAST | 72 | |
Repeati | 86 – 157 | Annexin 2PROSITE-ProRule annotationAdd BLAST | 72 | |
Repeati | 169 – 241 | Annexin 3PROSITE-ProRule annotationAdd BLAST | 73 | |
Repeati | 245 – 316 | Annexin 4PROSITE-ProRule annotationAdd BLAST | 72 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Annexin, RepeatPhylogenomic databases
eggNOGi | KOG0819, Eukaryota |
GeneTreei | ENSGT00940000159797 |
HOGENOMi | CLU_025300_0_2_1 |
InParanoidi | P27216 |
OMAi | MGNHHAK |
PhylomeDBi | P27216 |
TreeFami | TF105452 |
Family and domain databases
Gene3Di | 1.10.220.10, 4 hits |
InterProi | View protein in InterPro IPR001464, Annexin IPR018502, Annexin_repeat IPR018252, Annexin_repeat_CS IPR037104, Annexin_sf IPR009166, ANX13 |
Pfami | View protein in Pfam PF00191, Annexin, 4 hits |
PRINTSi | PR00196, ANNEXIN PR01811, ANNEXINXIII |
SMARTi | View protein in SMART SM00335, ANX, 4 hits |
SUPFAMi | SSF47874, SSF47874, 1 hit |
PROSITEi | View protein in PROSITE PS00223, ANNEXIN_1, 3 hits PS51897, ANNEXIN_2, 4 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNRHAKASS PQGFDVDRDA KKLNKACKGM GTNEAAIIEI LSGRTSDERQ
60 70 80 90 100
QIKQKYKATY GKELEEVLKS ELSGNFEKTA LALLDRPSEY AARQLQKAMK
110 120 130 140 150
GLGTDESVLI EVLCTRTNKE IIAIKEAYQR LFDRSLESDV KGDTSGNLKK
160 170 180 190 200
ILVSLLQANR NEGDDVDKDL AGQDAKDLYD AGEGRWGTDE LAFNEVLAKR
210 220 230 240 250
SYKQLRATFQ AYQILIGKDI EEAIEEETSG DLQKAYLTLV RCAQDCEDYF
260 270 280 290 300
AERLYKSMKG AGTDEETLIR IVVTRAEVDL QGIKAKFQEK YQKSLSDMVR
310
SDTSGDFRKL LVALLH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE5RIN3 | E5RIN3_HUMAN | Annexin A13 | ANXA13 | 113 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | V → F in CAA77578 (PubMed:1530946).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_055501 | 86 | R → H. Corresponds to variant dbSNP:rs2294013Ensembl. | 1 | |
Natural variantiVAR_055502 | 108 | V → I. Corresponds to variant dbSNP:rs6995099Ensembl. | 1 | |
Natural variantiVAR_055503 | 272 | V → I. Corresponds to variant dbSNP:rs2294015Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000291 | 5 | H → HSQSYTLSEGSQQLPKGDSQ PSTVVQPLSHPSRNGEPEAP QP in isoform B. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11502 mRNA Translation: CAA77578.1 AJ306450 mRNA Translation: CAC34622.1 AC135166 Genomic DNA No translation available. |
CCDSi | CCDS34939.1 [P27216-2] CCDS47917.1 [P27216-1] |
PIRi | A41733, LUHUIS |
RefSeqi | NP_001003954.1, NM_001003954.2 [P27216-2] NP_004297.2, NM_004306.3 [P27216-1] |
Genome annotation databases
Ensembli | ENST00000262219; ENSP00000262219; ENSG00000104537 [P27216-2] ENST00000419625; ENSP00000390809; ENSG00000104537 |
GeneIDi | 312 |
KEGGi | hsa:312 |
MANE-Selecti | ENST00000419625.6; ENSP00000390809.1; NM_004306.4; NP_004297.2 |
UCSCi | uc003yqt.4, human [P27216-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11502 mRNA Translation: CAA77578.1 AJ306450 mRNA Translation: CAC34622.1 AC135166 Genomic DNA No translation available. |
CCDSi | CCDS34939.1 [P27216-2] CCDS47917.1 [P27216-1] |
PIRi | A41733, LUHUIS |
RefSeqi | NP_001003954.1, NM_001003954.2 [P27216-2] NP_004297.2, NM_004306.3 [P27216-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6B3I | X-ray | 2.60 | A/B/C | 1-316 | [»] | |
SMRi | P27216 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106809, 19 interactors |
IntActi | P27216, 6 interactors |
STRINGi | 9606.ENSP00000262219 |
PTM databases
GlyGeni | P27216, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P27216 |
PhosphoSitePlusi | P27216 |
Genetic variation databases
BioMutai | ANXA13 |
DMDMi | 281185504 |
Proteomic databases
EPDi | P27216 |
jPOSTi | P27216 |
MassIVEi | P27216 |
MaxQBi | P27216 |
PaxDbi | P27216 |
PeptideAtlasi | P27216 |
PRIDEi | P27216 |
ProteomicsDBi | 54377 [P27216-1] 54378 [P27216-2] |
Protocols and materials databases
Antibodypediai | 13866, 222 antibodies from 29 providers |
DNASUi | 312 |
Genome annotation databases
Ensembli | ENST00000262219; ENSP00000262219; ENSG00000104537 [P27216-2] ENST00000419625; ENSP00000390809; ENSG00000104537 |
GeneIDi | 312 |
KEGGi | hsa:312 |
MANE-Selecti | ENST00000419625.6; ENSP00000390809.1; NM_004306.4; NP_004297.2 |
UCSCi | uc003yqt.4, human [P27216-1] |
Organism-specific databases
CTDi | 312 |
DisGeNETi | 312 |
GeneCardsi | ANXA13 |
HGNCi | HGNC:536, ANXA13 |
HPAi | ENSG00000104537, Tissue enhanced (gallbladder, intestine) |
MIMi | 602573, gene |
neXtProti | NX_P27216 |
OpenTargetsi | ENSG00000104537 |
PharmGKBi | PA24826 |
VEuPathDBi | HostDB:ENSG00000104537 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0819, Eukaryota |
GeneTreei | ENSGT00940000159797 |
HOGENOMi | CLU_025300_0_2_1 |
InParanoidi | P27216 |
OMAi | MGNHHAK |
PhylomeDBi | P27216 |
TreeFami | TF105452 |
Enzyme and pathway databases
PathwayCommonsi | P27216 |
SignaLinki | P27216 |
Miscellaneous databases
BioGRID-ORCSi | 312, 10 hits in 1031 CRISPR screens |
GeneWikii | ANXA13 |
GenomeRNAii | 312 |
Pharosi | P27216, Tbio |
PROi | PR:P27216 |
RNActi | P27216, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000104537, Expressed in intestine and 106 other tissues |
ExpressionAtlasi | P27216, baseline and differential |
Genevisiblei | P27216, HS |
Family and domain databases
Gene3Di | 1.10.220.10, 4 hits |
InterProi | View protein in InterPro IPR001464, Annexin IPR018502, Annexin_repeat IPR018252, Annexin_repeat_CS IPR037104, Annexin_sf IPR009166, ANX13 |
Pfami | View protein in Pfam PF00191, Annexin, 4 hits |
PRINTSi | PR00196, ANNEXIN PR01811, ANNEXINXIII |
SMARTi | View protein in SMART SM00335, ANX, 4 hits |
SUPFAMi | SSF47874, SSF47874, 1 hit |
PROSITEi | View protein in PROSITE PS00223, ANNEXIN_1, 3 hits PS51897, ANNEXIN_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ANX13_HUMAN | |
Accessioni | P27216Primary (citable) accession number: P27216 Secondary accession number(s): Q9BQR5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 15, 2009 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families