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UniProtKB - P27099 (TCBD_PSESQ)
Protein
Chloromuconate cycloisomerase
Gene
tcbD
Organism
Pseudomonas sp. (strain P51)
Status
Functioni
Miscellaneous
Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.
Catalytic activityi
- EC:5.5.1.7
Cofactori
Mn2+1 Publication
: 3-chlorocatechol degradation Pathwayi
This protein is involved in the pathway 3-chlorocatechol degradation, which is part of Aromatic compound metabolism.View all proteins of this organism that are known to be involved in the pathway 3-chlorocatechol degradation and in Aromatic compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 165 | Proton acceptor | 1 | |
Metal bindingi | 194 | Manganese | 1 | |
Metal bindingi | 220 | Manganese | 1 | |
Metal bindingi | 245 | Manganese | 1 | |
Active sitei | 323 | Proton donor | 1 |
GO - Molecular functioni
- chloromuconate cycloisomerase activity Source: UniProtKB
- manganese ion binding Source: InterPro
- muconate cycloisomerase activity Source: InterPro
GO - Biological processi
- 2,4-dichlorophenoxyacetic acid catabolic process Source: UniProtKB
- cellular amino acid catabolic process Source: InterPro
Keywordsi
Molecular function | Isomerase |
Biological process | Aromatic hydrocarbons catabolism |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00083 |
Names & Taxonomyi
Protein namesi | Recommended name: Chloromuconate cycloisomerase (EC:5.5.1.7)Alternative name(s): Muconate cycloisomerase II |
Gene namesi | Name:tcbD |
Encoded oni | Plasmid pP51 |
Organismi | Pseudomonas sp. (strain P51) |
Taxonomic identifieri | 65067 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171254 | 1 – 370 | Chloromuconate cycloisomeraseAdd BLAST | 370 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27099 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27099 |
Family & Domainsi
Sequence similaritiesi
Belongs to the mandelate racemase/muconate lactonizing enzyme family.Curated
Family and domain databases
CDDi | cd03318, MLE, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR013370, Chloromuconate_cycloisomerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDG01258, (chloro)muconate_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR02534, mucon_cyclo, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P27099-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIEAISTTI VDVPTRRPLQ MSFTTVHKQS YVIVQVKAGG LVGIGEGGSV
60 70 80 90 100
GGPTWGSESA ETIKVIIDNY LAPLLVGKDA SNLSQARVLM DRAVTGNLSA
110 120 130 140 150
KAAIDIALHD LKARALNLSI ADLIGGTMRT SIPIAWTLAS GDTARDIDSA
160 170 180 190 200
LEMIETRRHN RFKVKLGART PAQDLEHIRS IVKAVGDRAS VRVDVNQGWD
210 220 230 240 250
EQTASIWIPR LEEAGVELVE QPVPRANFGA LRRLTEQNGV AILADESLSS
260 270 280 290 300
LSSAFELARD HAVDAFSLKL CNMGGIANTL KVAAVAEAAG ISSYGGTMLD
310 320 330 340 350
STVGTAAALH VYATLPSLPY GCELIGPWVL GDRLTQQDLE IKDFEVHLPL
360 370
GSGLGVDLDH DKVRHYTRAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57629 Genomic DNA Translation: AAD13626.1 |
PIRi | B43673 |
Genome annotation databases
KEGGi | ag:AAD13626 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M57629 Genomic DNA Translation: AAD13626.1 |
PIRi | B43673 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NU5 | X-ray | 1.95 | A | 1-370 | [»] | |
SMRi | P27099 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
KEGGi | ag:AAD13626 |
Enzyme and pathway databases
UniPathwayi | UPA00083 |
Miscellaneous databases
EvolutionaryTracei | P27099 |
Family and domain databases
CDDi | cd03318, MLE, 1 hit |
Gene3Di | 3.20.20.120, 1 hit 3.30.390.10, 1 hit |
InterProi | View protein in InterPro IPR013370, Chloromuconate_cycloisomerase IPR036849, Enolase-like_C_sf IPR029017, Enolase-like_N IPR029065, Enolase_C-like IPR018110, Mandel_Rmase/mucon_lact_enz_CS IPR013342, Mandelate_racemase_C IPR013341, Mandelate_racemase_N_dom |
Pfami | View protein in Pfam PF13378, MR_MLE_C, 1 hit PF02746, MR_MLE_N, 1 hit |
SFLDi | SFLDG01258, (chloro)muconate_cycloisomeras, 1 hit |
SMARTi | View protein in SMART SM00922, MR_MLE, 1 hit |
SUPFAMi | SSF51604, SSF51604, 1 hit SSF54826, SSF54826, 1 hit |
TIGRFAMsi | TIGR02534, mucon_cyclo, 1 hit |
PROSITEi | View protein in PROSITE PS00908, MR_MLE_1, 1 hit PS00909, MR_MLE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TCBD_PSESQ | |
Accessioni | P27099Primary (citable) accession number: P27099 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | February 23, 2022 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, PlasmidDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families