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Protein

Transforming growth factor beta-2 proprotein

Gene

Tgfb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.By similarity
Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.By similarity
Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development (By similarity). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGrowth factor, Mitogen

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-2129379 Molecules associated with elastic fibres

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming growth factor beta-2 proprotein
Cleaved into the following 2 chains:
Gene namesi
Name:Tgfb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:98726 Tgfb2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003378621 – 302Latency-associated peptideBy similarityAdd BLAST282
ChainiPRO_0000033787303 – 414Transforming growth factor beta-2By similarityAdd BLAST112

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi140N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi309 ↔ 318By similarity
Disulfide bondi317 ↔ 380By similarity
Disulfide bondi346 ↔ 411By similarity
Disulfide bondi350 ↔ 413By similarity
Disulfide bondi379InterchainBy similarity

Post-translational modificationi

Transforming growth factor beta-2 proprotein: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP27090
PaxDbiP27090
PeptideAtlasiP27090
PRIDEiP27090

PTM databases

iPTMnetiP27090
PhosphoSitePlusiP27090

Expressioni

Gene expression databases

BgeeiENSMUSG00000039239 Expressed in 378 organ(s), highest expression level in uterus
CleanExiMM_TGFB2
ExpressionAtlasiP27090 baseline and differential
GenevisibleiP27090 MM

Interactioni

Subunit structurei

Interacts with the serine proteases, HTRA1 and HTRA3 (PubMed:15206957, PubMed:14973287). Interacts with ASPN (By similarity). Interacts with MFAP5 (PubMed:23963447). Latency-associated peptide: Interacts with Transforming growth factor beta-2 (TGF-beta-2) chain; interaction is non-covalent and maintains (TGF-beta-2) in a latent state (By similarity). Latency-associated peptide: Interacts with LRRC32/GARP; leading to regulate activation of TGF-beta-2 (By similarity). Latency-associated peptide: Interacts with NREP; the interaction results in a decrease in TGFB2 autoinduction (PubMed:14985127). Transforming growth factor beta-2: Homodimer; disulfide-linked (By similarity). Transforming growth factor beta-2: Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction (By similarity).By similarity4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204160, 2 interactors
ComplexPortaliCPX-827 TGF-beta-2 complex
CPX-836 TGF-beta-2-TGFR complex
IntActiP27090, 1 interactor
MINTiP27090
STRINGi10090.ENSMUSP00000043849

Structurei

Secondary structure

1414
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP27090
SMRiP27090
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900 Eukaryota
ENOG410XT8Z LUCA
GeneTreeiENSGT00910000143982
HOGENOMiHOG000290198
HOVERGENiHBG074115
InParanoidiP27090
KOiK13376
OMAiWSADTQH
TreeFamiTF318514

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR001839 TGF-b_C
IPR001111 TGF-b_propeptide
IPR016319 TGF-beta
IPR015615 TGF-beta-rel
IPR003940 TGFb2
IPR017948 TGFb_CS
PANTHERiPTHR11848 PTHR11848, 1 hit
PTHR11848:SF141 PTHR11848:SF141, 1 hit
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PF00688 TGFb_propeptide, 1 hit
PIRSFiPIRSF001787 TGF-beta, 1 hit
PRINTSiPR01423 TGFBETA
PR01425 TGFBETA2
SMARTiView protein in SMART
SM00204 TGFB, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00250 TGF_BETA_1, 1 hit
PS51362 TGF_BETA_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P27090-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHYCVLSTFL LLHLVPVALS LSTCSTLDMD QFMRKRIEAI RGQILSKLKL
60 70 80 90 100
TSPPEDYPEP DEVPPEVISI YNSTRDLLQE KASRRAAACE RERSDEEYYA
110 120 130 140 150
KEVYKIDMPS HLPSENAIPP TFYRPYFRIV RFDVSTMEKN ASNLVKAEFR
160 170 180 190 200
VFRLQNPKAR VAEQRIELYQ ILKSKDLTSP TQRYIDSKVV KTRAEGEWLS
210 220 230 240 250
FDVTDAVQEW LHHKDRNLGF KISLHCPCCT FVPSNNYIIP NKSEELEARF
260 270 280 290 300
AGIDGTSTYA SGDQKTIKST RKKTSGKTPH LLLMLLPSYR LESQQSSRRK
310 320 330 340 350
KRALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC
360 370 380 390 400
PYLWSSDTQH TKVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGNTPKI
410
EQLSNMIVKS CKCS
Length:414
Mass (Da):47,589
Last modified:July 27, 2011 - v2
Checksum:iDB37A7C38881F286
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YXU9A0A0A6YXU9_MOUSE
Transforming growth factor beta
Tgfb2
442Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95 – 96DE → EQ in CAA40672 (PubMed:2797004).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57413 mRNA Translation: CAA40672.1
BC011170 mRNA Translation: AAH11170.1
CCDSiCCDS15601.1
PIRiA40148 WFMSB2
RefSeqiNP_033393.2, NM_009367.4
UniGeneiMm.18213

Genome annotation databases

EnsembliENSMUST00000045288; ENSMUSP00000043849; ENSMUSG00000039239
GeneIDi21808
KEGGimmu:21808
UCSCiuc007dzo.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57413 mRNA Translation: CAA40672.1
BC011170 mRNA Translation: AAH11170.1
CCDSiCCDS15601.1
PIRiA40148 WFMSB2
RefSeqiNP_033393.2, NM_009367.4
UniGeneiMm.18213

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TX2X-ray1.82A/B303-414[»]
5TX6X-ray2.75A/B/C303-414[»]
ProteinModelPortaliP27090
SMRiP27090
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204160, 2 interactors
ComplexPortaliCPX-827 TGF-beta-2 complex
CPX-836 TGF-beta-2-TGFR complex
IntActiP27090, 1 interactor
MINTiP27090
STRINGi10090.ENSMUSP00000043849

PTM databases

iPTMnetiP27090
PhosphoSitePlusiP27090

Proteomic databases

MaxQBiP27090
PaxDbiP27090
PeptideAtlasiP27090
PRIDEiP27090

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045288; ENSMUSP00000043849; ENSMUSG00000039239
GeneIDi21808
KEGGimmu:21808
UCSCiuc007dzo.2 mouse

Organism-specific databases

CTDi7042
MGIiMGI:98726 Tgfb2

Phylogenomic databases

eggNOGiKOG3900 Eukaryota
ENOG410XT8Z LUCA
GeneTreeiENSGT00910000143982
HOGENOMiHOG000290198
HOVERGENiHBG074115
InParanoidiP27090
KOiK13376
OMAiWSADTQH
TreeFamiTF318514

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-2129379 Molecules associated with elastic fibres

Miscellaneous databases

ChiTaRSiTgfb2 mouse
PROiPR:P27090
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039239 Expressed in 378 organ(s), highest expression level in uterus
CleanExiMM_TGFB2
ExpressionAtlasiP27090 baseline and differential
GenevisibleiP27090 MM

Family and domain databases

Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR001839 TGF-b_C
IPR001111 TGF-b_propeptide
IPR016319 TGF-beta
IPR015615 TGF-beta-rel
IPR003940 TGFb2
IPR017948 TGFb_CS
PANTHERiPTHR11848 PTHR11848, 1 hit
PTHR11848:SF141 PTHR11848:SF141, 1 hit
PfamiView protein in Pfam
PF00019 TGF_beta, 1 hit
PF00688 TGFb_propeptide, 1 hit
PIRSFiPIRSF001787 TGF-beta, 1 hit
PRINTSiPR01423 TGFBETA
PR01425 TGFBETA2
SMARTiView protein in SMART
SM00204 TGFB, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00250 TGF_BETA_1, 1 hit
PS51362 TGF_BETA_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTGFB2_MOUSE
AccessioniPrimary (citable) accession number: P27090
Secondary accession number(s): Q91VP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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