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Protein

Beta-glucanase

Gene

bg1

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134Nucleophile1
Active sitei138Proton donor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.73 669
SABIO-RKiP27051

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bg1
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51D → N: 30% of wild-type activity. 1
Mutagenesisi89D → N: 85% of wild-type activity. 1
Mutagenesisi92E → Q: 3% of wild-type activity. 1
Mutagenesisi105E → Q: 50% of wild-type activity. 1
Mutagenesisi133D → N: 15% of wild-type activity. 1
Mutagenesisi134E → Q: 0.2% of wild-type activity. 1
Mutagenesisi136D → N: 0.5% of wild-type activity. 1
Mutagenesisi138E → Q: Complete loss of activity. 1
Mutagenesisi143D → N: 65% of wild-type activity. 1
Mutagenesisi160E → Q: 15% of wild-type activity. 1
Mutagenesisi168D → N: 60% of wild-type activity. 1
Mutagenesisi179D → N: 80% of wild-type activity. 1
Mutagenesisi190D → N: 70% of wild-type activity. 1
Mutagenesisi219D → N: No change in activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000001178828 – 243Beta-glucanaseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi61 ↔ 901 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1243
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP27051
SMRiP27051
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27051

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 243GH16PROSITE-ProRule annotationAdd BLAST215

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR008264 Beta_glucanase
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PRINTSiPR00737 GLHYDRLASE16
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27051-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSYRVKRMLM LLVTGLFLSL STFAASASAQ TGGSFYEPFN NYNTGLWQKA
60 70 80 90 100
DGYSNGNMFN CTWRANNVSM TSLGEMRLSL TSPSYNKFDC GENRSVQTYG
110 120 130 140 150
YGLYEVNMKP AKNVGIVSSF FTYTGPTDGT PWDEIDIEFL GKDTTKVQFN
160 170 180 190 200
YYTNGVGNHE KIVNLGFDAA NSYHTYAFDW QPNSIKWYVD GQLKHTATTQ
210 220 230 240
IPQTPGKIMM NLWNGAGVDE WLGSYNGVTP LSRSLHWVRY TKR
Length:243
Mass (Da):27,435
Last modified:August 1, 1992 - v1
Checksum:i651188D9AAD609A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232S → Y (PubMed:7589539).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57279 Genomic DNA Translation: CAA40547.1
PIRiS15388

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57279 Genomic DNA Translation: CAA40547.1
PIRiS15388

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GBGX-ray1.80A30-243[»]
3D6EX-ray2.40A/B30-243[»]
ProteinModelPortaliP27051
SMRiP27051
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.1.73 669
SABIO-RKiP27051

Miscellaneous databases

EvolutionaryTraceiP27051

Family and domain databases

InterProiView protein in InterPro
IPR008264 Beta_glucanase
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PRINTSiPR00737 GLHYDRLASE16
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGUB_BACLI
AccessioniPrimary (citable) accession number: P27051
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 22, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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