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UniProtKB - P27035 (GUNA_STRLI)
Protein
Endoglucanase CelA
Gene
celA
Organism
Streptomyces lividans
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. EC:3.2.1.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 286 | Proton donorBy similarity | 1 | |
Active sitei | 378 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- cellulase activity Source: UniProtKB-EC
- polysaccharide binding Source: InterPro
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 GH5, Glycoside Hydrolase Family 5 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase CelA (EC:3.2.1.4)Alternative name(s): Cellulase Endo-1,4-beta-glucanase |
Gene namesi | Name:celA |
Organismi | Streptomyces lividans |
Taxonomic identifieri | 1916 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Streptomycetales › Streptomycetaceae › Streptomyces |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000007873 | 28 – 459 | Endoglucanase CelAAdd BLAST | 432 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 31 ↔ 131 | By similarity |
Post-translational modificationi
The linker region (also termed 'hinge') may be a potential site for proteolysis.
Keywords - PTMi
Disulfide bondFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 134 | CBM2PROSITE-ProRule annotationAdd BLAST | 107 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 129 – 151 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 136 – 147 | Linker ('hinge') (Pro-Thr box)Add BLAST | 12 | |
Regioni | 148 – 357 | CatalyticAdd BLAST | 210 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 2.60.40.290, 1 hit |
InterProi | View protein in InterPro IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF00150, Cellulase, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit |
SUPFAMi | SSF49384, SSF49384, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P27035-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLLALLAT GVSIVGLTAL AGPPAQAATG CKAEYTITSQ WEGGFQAGVK
60 70 80 90 100
ITNLGDPVSG WTLGFTMPDA GQRLVQGWNA TWSQSGSAVT AGGVDWNRTL
110 120 130 140 150
ATGASADLGF VGSFTGANPA PTSFTLNGAT CSGSVTDPPT DPPTDPPATG
160 170 180 190 200
TPAAVNGQLH VCGVHLCNQY DRPIQLRGMS THGIQWFGPC YGDASLDRLA
210 220 230 240 250
QDWKSDLLRV AMYVQEDGYE TDPAGFTSRV NGLVDMAEDR GMYAVIDFHT
260 270 280 290 300
LTPGDPNYNL DRARTFFSSV AARNDKKNVI YEIANEPNGV SWTAVKSYAE
310 320 330 340 350
QVIPVIRAAD PDAVVIVGTR GWSSLGVSDG ANESEVVNNP VNATNIMYAF
360 370 380 390 400
HFYAASHKDD YRAAVRPAAT RLPLFVSEFG TVSATAWSVD RSSSVAWLDL
410 420 430 440 450
LDQLKISYAN WTYSDADEGS AAFRPGTCEG TDYSSSGVLT ESGALVKSRI
STTDDFPTS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M82807 Genomic DNA No translation available. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M82807 Genomic DNA No translation available. |
3D structure databases
SMRi | P27035 |
ModBasei | Search... |
Protein family/group databases
CAZyi | CBM2, Carbohydrate-Binding Module Family 2 GH5, Glycoside Hydrolase Family 5 |
Family and domain databases
Gene3Di | 2.60.40.290, 1 hit |
InterProi | View protein in InterPro IPR001919, CBD2 IPR008965, CBM2/CBM3_carb-bd_dom_sf IPR012291, CBM2_carb-bd_dom_sf IPR018366, CBM2_CS IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00553, CBM_2, 1 hit PF00150, Cellulase, 1 hit |
SMARTi | View protein in SMART SM00637, CBD_II, 1 hit |
SUPFAMi | SSF49384, SSF49384, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS51173, CBM2, 1 hit PS00561, CBM2_A, 1 hit PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GUNA_STRLI | |
Accessioni | P27035Primary (citable) accession number: P27035 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | June 2, 2021 | |
This is version 105 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - SIMILARITY comments
Index of protein domains and families