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UniProtKB - P27017 (AMIC_PSEAE)
Protein
Aliphatic amidase expression-regulating protein
Gene
amiC
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Functioni
Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity.
GO - Molecular functioni
- amide binding Source: PseudoCAP
- kinase activity Source: UniProtKB-KW
GO - Biological processi
- amino acid transport Source: InterPro
- negative regulation of hydrolase activity Source: PseudoCAP
- regulation of cellular amide catabolic process Source: PseudoCAP
Keywordsi
Molecular function | Kinase, Repressor, Transferase |
Names & Taxonomyi
Protein namesi | Recommended name: Aliphatic amidase expression-regulating protein |
Gene namesi | Name:amiC Ordered Locus Names:PA3364 |
Organismi | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) |
Taxonomic identifieri | 208964 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
Organism-specific databases
PseudoCAPi | PA3364 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000064581 | 2 – 385 | Aliphatic amidase expression-regulating proteinAdd BLAST | 384 |
Proteomic databases
PaxDbi | P27017 |
Interactioni
Subunit structurei
Homodimer.
Forms a complex with AmiR.
Protein-protein interaction databases
IntActi | P27017, 1 interactor |
STRINGi | 287.DR97_4563 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P27017 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P27017 |
Family & Domainsi
Domaini
Consists of two beta-alpha-beta domains with a central cleft in which the amide binds.
Phylogenomic databases
HOGENOMi | CLU_027128_1_1_6 |
InParanoidi | P27017 |
OMAi | YPYESNR |
PhylomeDBi | P27017 |
Family and domain databases
CDDi | cd06357, PBP1_AmiC, 1 hit |
InterProi | View protein in InterPro IPR039570, AmiC_PBP1 IPR000709, Leu_Ile_Val-bd IPR028082, Peripla_BP_I |
PRINTSi | PR00337, LEUILEVALBP |
SUPFAMi | SSF53822, SSF53822, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P27017-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSHQERPLI GLLFSETGVT ADIERSQRYG ALLAVEQLNR EGGVGGRPIE
60 70 80 90 100
TLSQDPGGDP DRYRLCAEDF IRNRGVRFLV GCYMSHTRKA VMPVVERADA
110 120 130 140 150
LLCYPTPYEG FEYSPNIVYG GPAPNQNSAP LAAYLIRHYG ERVVFIGSDY
160 170 180 190 200
IYPRESNHVM RHLYRQHGGT VLEEIYIPLY PSDDDVQRAV ERIYQARADV
210 220 230 240 250
VFSTVVGTGT AELYRAIARR YGDGRRPPIA SLTTSEAEVA KMESDVAEGQ
260 270 280 290 300
VVVAPYFSSI DTAASRAFVQ ACHGFFPENA TITAWAEAAY WQTLLLGRAA
310 320 330 340 350
QAAGSWRVED VQRHLYDICI DAPQGPVRVE RQNNHSRLSS RIAEIDARGV
360 370 380
FQVRWQSPEP IRPDPYVVVH NLDDWSASMG GGALP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 – 28 | QR → HA in CAA32024 (PubMed:1907262).Curated | 2 | |
Sequence conflicti | 186 | V → L in CAA32024 (PubMed:1907262).Curated | 1 | |
Sequence conflicti | 263 | A → P in CAA32024 (PubMed:1907262).Curated | 1 | |
Sequence conflicti | 305 | S → N in CAA32024 (PubMed:1907262).Curated | 1 | |
Sequence conflicti | 319 | C → D in CAA32024 (PubMed:1907262).Curated | 1 | |
Sequence conflicti | 383 | A → P in CAA32024 (PubMed:1907262).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 106 | T → N in strain: PAC181; butyramide inducible phenotype. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13776 Genomic DNA Translation: CAA32024.1 AE004091 Genomic DNA Translation: AAG06752.1 |
PIRi | A40359 C83226 |
RefSeqi | NP_252054.1, NC_002516.2 WP_003091751.1, NZ_QZGE01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAG06752; AAG06752; PA3364 |
GeneIDi | 877798 |
KEGGi | pae:PA3364 |
PATRICi | fig|208964.12.peg.3523 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13776 Genomic DNA Translation: CAA32024.1 AE004091 Genomic DNA Translation: AAG06752.1 |
PIRi | A40359 C83226 |
RefSeqi | NP_252054.1, NC_002516.2 WP_003091751.1, NZ_QZGE01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1PEA | X-ray | 2.10 | A | 1-385 | [»] | |
1QNL | X-ray | 2.70 | A | 1-375 | [»] | |
1QO0 | X-ray | 2.25 | A/B | 1-380 | [»] | |
SMRi | P27017 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P27017, 1 interactor |
STRINGi | 287.DR97_4563 |
Chemistry databases
DrugBanki | DB02736, Acetamide DB02121, Butyramide |
Proteomic databases
PaxDbi | P27017 |
Genome annotation databases
EnsemblBacteriai | AAG06752; AAG06752; PA3364 |
GeneIDi | 877798 |
KEGGi | pae:PA3364 |
PATRICi | fig|208964.12.peg.3523 |
Organism-specific databases
PseudoCAPi | PA3364 |
Phylogenomic databases
HOGENOMi | CLU_027128_1_1_6 |
InParanoidi | P27017 |
OMAi | YPYESNR |
PhylomeDBi | P27017 |
Miscellaneous databases
EvolutionaryTracei | P27017 |
Family and domain databases
CDDi | cd06357, PBP1_AmiC, 1 hit |
InterProi | View protein in InterPro IPR039570, AmiC_PBP1 IPR000709, Leu_Ile_Val-bd IPR028082, Peripla_BP_I |
PRINTSi | PR00337, LEUILEVALBP |
SUPFAMi | SSF53822, SSF53822, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMIC_PSEAE | |
Accessioni | P27017Primary (citable) accession number: P27017 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references