UniProtKB - P27005 (S10A8_MOUSE)
Protein S100-A8
S100a8
Functioni
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 17 | ZincBy similarity | 1 | |
Metal bindingi | 27 | ZincBy similarity | 1 | |
Metal bindingi | 83 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 20 – 33 | 1; low affinityPROSITE-ProRule annotationAdd BLAST | 14 | |
Calcium bindingi | 59 – 70 | 2; high affinityPROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- antioxidant activity Source: UniProtKB-KW
- arachidonic acid binding Source: InterPro
- calcium-dependent protein binding Source: GO_Central
- calcium ion binding Source: GO_Central
- microtubule binding Source: InterPro
- RAGE receptor binding Source: InterPro
- Toll-like receptor 4 binding Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
- apoptotic process Source: UniProtKB-KW
- astrocyte development Source: MGI
- autocrine signaling Source: MGI
- autophagy Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- leukocyte migration involved in inflammatory response Source: UniProtKB
- neutrophil aggregation Source: UniProtKB
- neutrophil chemotaxis Source: UniProtKB
- peptidyl-cysteine S-nitrosylation Source: MGI
- positive regulation of inflammatory response Source: UniProtKB
- positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
- positive regulation of peptide secretion Source: MGI
- response to lipopolysaccharide Source: GO_Central
Keywordsi
Molecular function | Antimicrobial, Antioxidant |
Biological process | Apoptosis, Autophagy, Chemotaxis, Immunity, Inflammatory response, Innate immunity |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-5668599, RHO GTPases Activate NADPH Oxidases R-MMU-5686938, Regulation of TLR by endogenous ligand R-MMU-6798695, Neutrophil degranulation R-MMU-6799990, Metal sequestration by antimicrobial proteins |
Names & Taxonomyi
Protein namesi | Recommended name: Protein S100-A8Alternative name(s): Calgranulin-A Chemotactic cytokine CP-10 Leukocyte L1 complex light chain Migration inhibitory factor-related protein 8 Short name: MRP-8 Short name: p8 Pro-inflammatory S100 cytokine S100 calcium-binding protein A8 |
Gene namesi | Name:S100a8 Synonyms:Caga, Mrp8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88244, S100a8 |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Cytoskeleton
- cytoskeleton By similarity
Other locations
- Cytoplasm By similarity
Note: Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway.
Cytoskeleton
- intermediate filament cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000143994 | 2 – 89 | Protein S100-A8Add BLAST | 88 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 42 | S-nitrosocysteine1 Publication | 1 |
Keywords - PTMi
S-nitrosylationProteomic databases
PaxDbi | P27005 |
PeptideAtlasi | P27005 |
PRIDEi | P27005 |
PTM databases
iPTMneti | P27005 |
PhosphoSitePlusi | P27005 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000056054, Expressed in granulocyte and 178 other tissues |
Genevisiblei | P27005, MM |
Interactioni
Subunit structurei
Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A9 known as calprotectin (S100A8/A9). Calprotectin (S100A8/9) interacts with CEACAM3 and tubulin filaments in a calcium-dependent manner. Heterotetrameric calprotectin (S100A8/A9) interacts with ANXA6 and associates with tubulin filaments in activated monocytes. S100A8 and calprotectin (S100A8/9) interact with NCF2/P67PHOX, RAC1 and RAC2. Calprotectin (S100A8/9) interacts with CYBA and CYBB (By similarity). S100A8 interacts with AGER, ATP2A2 and with the heterodimeric complex formed by TLR4 and LY96. Calprotectin (S100A8/9) interacts with NOS2 to form the iNOS-S100A8/A9 transnitrosylase complex (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- calcium-dependent protein binding Source: GO_Central
- microtubule binding Source: InterPro
- RAGE receptor binding Source: InterPro
- Toll-like receptor 4 binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 203056, 1 interactor |
ComplexPortali | CPX-40, Calprotectin heterotetramer CPX-41, Calprotectin heterodimer CPX-47, S100A8 complex CPX-53, iNOS-S100A8/A9 complex |
IntActi | P27005, 3 interactors |
MINTi | P27005 |
STRINGi | 10090.ENSMUSP00000064385 |
Miscellaneous databases
RNActi | P27005, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 13 – 48 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 46 – 81 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502SA01, Eukaryota |
GeneTreei | ENSGT00910000144329 |
HOGENOMi | CLU_138624_6_0_1 |
InParanoidi | P27005 |
OMAi | NYHAIYR |
OrthoDBi | 1613717at2759 |
PhylomeDBi | P27005 |
TreeFami | TF332727 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR001751, S100/CaBP-9k_CS IPR013787, S100_Ca-bd_sub IPR028474, S100A8 |
PANTHERi | PTHR11639:SF5, PTHR11639:SF5, 1 hit |
Pfami | View protein in Pfam PF01023, S_100, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 1 hit SM01394, S_100, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS00303, S100_CABP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN
60 70 80
IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 57 | E → D in CAA61204 (PubMed:8611640).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83218 mRNA Translation: AAB07229.1 S57123 mRNA Translation: AAB25840.1 X87966 Genomic DNA Translation: CAA61204.1 BC078629 mRNA Translation: AAH78629.1 |
CCDSi | CCDS38507.1 |
PIRi | I56163 |
RefSeqi | NP_038678.1, NM_013650.2 |
Genome annotation databases
Ensembli | ENSMUST00000069927; ENSMUSP00000064385; ENSMUSG00000056054 |
GeneIDi | 20201 |
KEGGi | mmu:20201 |
UCSCi | uc008qdd.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M83218 mRNA Translation: AAB07229.1 S57123 mRNA Translation: AAB25840.1 X87966 Genomic DNA Translation: CAA61204.1 BC078629 mRNA Translation: AAH78629.1 |
CCDSi | CCDS38507.1 |
PIRi | I56163 |
RefSeqi | NP_038678.1, NM_013650.2 |
3D structure databases
SMRi | P27005 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203056, 1 interactor |
ComplexPortali | CPX-40, Calprotectin heterotetramer CPX-41, Calprotectin heterodimer CPX-47, S100A8 complex CPX-53, iNOS-S100A8/A9 complex |
IntActi | P27005, 3 interactors |
MINTi | P27005 |
STRINGi | 10090.ENSMUSP00000064385 |
PTM databases
iPTMneti | P27005 |
PhosphoSitePlusi | P27005 |
Proteomic databases
PaxDbi | P27005 |
PeptideAtlasi | P27005 |
PRIDEi | P27005 |
Protocols and materials databases
Antibodypediai | 3463, 1286 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000069927; ENSMUSP00000064385; ENSMUSG00000056054 |
GeneIDi | 20201 |
KEGGi | mmu:20201 |
UCSCi | uc008qdd.1, mouse |
Organism-specific databases
CTDi | 6279 |
MGIi | MGI:88244, S100a8 |
Phylogenomic databases
eggNOGi | ENOG502SA01, Eukaryota |
GeneTreei | ENSGT00910000144329 |
HOGENOMi | CLU_138624_6_0_1 |
InParanoidi | P27005 |
OMAi | NYHAIYR |
OrthoDBi | 1613717at2759 |
PhylomeDBi | P27005 |
TreeFami | TF332727 |
Enzyme and pathway databases
Reactomei | R-MMU-5668599, RHO GTPases Activate NADPH Oxidases R-MMU-5686938, Regulation of TLR by endogenous ligand R-MMU-6798695, Neutrophil degranulation R-MMU-6799990, Metal sequestration by antimicrobial proteins |
Miscellaneous databases
BioGRID-ORCSi | 20201, 1 hit in 15 CRISPR screens |
PROi | PR:P27005 |
RNActi | P27005, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000056054, Expressed in granulocyte and 178 other tissues |
Genevisiblei | P27005, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR001751, S100/CaBP-9k_CS IPR013787, S100_Ca-bd_sub IPR028474, S100A8 |
PANTHERi | PTHR11639:SF5, PTHR11639:SF5, 1 hit |
Pfami | View protein in Pfam PF01023, S_100, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 1 hit SM01394, S_100, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS00303, S100_CABP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S10A8_MOUSE | |
Accessioni | P27005Primary (citable) accession number: P27005 Secondary accession number(s): P31724 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families