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Protein

Phosphoribosylamine--glycine ligase

Gene

purD

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi286Magnesium or manganeseUniRule annotation1
Metal bindingi288Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 196ATPUniRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSENT99287:G1FZD-4215-MONOMER
UniPathwayi
UPA00074;UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligaseUniRule annotation (EC:6.3.4.13UniRule annotation)
Alternative name(s):
GARSUniRule annotation
Glycinamide ribonucleotide synthetaseUniRule annotation
Phosphoribosylglycinamide synthetaseUniRule annotation
Gene namesi
Name:purDUniRule annotation
Ordered Locus Names:STM4175
ORF Names:STMF1.31
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001514771 – 429Phosphoribosylamine--glycine ligaseAdd BLAST429

Proteomic databases

PaxDbiP26977
PRIDEiP26977

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi99287.STM4175

Structurei

3D structure databases

ProteinModelPortaliP26977
SMRiP26977
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 316ATP-graspUniRule annotationAdd BLAST208

Sequence similaritiesi

Belongs to the GARS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C12 Bacteria
COG0151 LUCA
HOGENOMiHOG000033463
KOiK01945
OMAiKATVCKY
PhylomeDBiP26977

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

P26977-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVLVIGNGG REHALAWKAA QSPLVDTVFV APGNAGTALE PALQNVAIGV
60 70 80 90 100
TDIPALLSFA QHEKIDLTIV GPEAPLVIGV VDAFRAAGLK IFGPTEGAAQ
110 120 130 140 150
LEGSKAFTKD FLARHQIPTA EYQNFTEIEP ALAYLREKGA PIVIKADGLA
160 170 180 190 200
AGKGVIVAMT LEEAEAAVHD MLAGNAFGDA GHRIVIEEFL DGEEASFIVM
210 220 230 240 250
VDGEHVLPMA TSQDHKRVGN GDTGPNTGGM GAYSPAPVVT DEVHQRTMER
260 270 280 290 300
IIWPTVKGMA AEGNTYTGFL YAGLMIDKQG NPKVIEFNCR FGDPETQPIM
310 320 330 340 350
LRMKSDLVDL CLAACDGKLD EKTSEWDERA SLGVVIAAGG YPGSYSTGDE
360 370 380 390 400
IHGLPLEEVA DGKVFHAGTK LADDDRVLTS GGRVLCATAL GHTVAEAQKR
410 420
AYALMTDIRW DGSFSRNDIG WRAIEREQN
Length:429
Mass (Da):45,693
Last modified:August 1, 1992 - v1
Checksum:iA871C125417B5D41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66160 Genomic DNA Translation: AAA27198.1
AF170176 Genomic DNA Translation: AAF33521.1
AE006468 Genomic DNA Translation: AAL23003.1
PIRiS18489
RefSeqiNP_463044.1, NC_003197.2
WP_000866767.1, NC_003197.2

Genome annotation databases

EnsemblBacteriaiAAL23003; AAL23003; STM4175
GeneIDi1255701
KEGGistm:STM4175
PATRICifig|99287.12.peg.4389

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M66160 Genomic DNA Translation: AAA27198.1
AF170176 Genomic DNA Translation: AAF33521.1
AE006468 Genomic DNA Translation: AAL23003.1
PIRiS18489
RefSeqiNP_463044.1, NC_003197.2
WP_000866767.1, NC_003197.2

3D structure databases

ProteinModelPortaliP26977
SMRiP26977
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM4175

Proteomic databases

PaxDbiP26977
PRIDEiP26977

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL23003; AAL23003; STM4175
GeneIDi1255701
KEGGistm:STM4175
PATRICifig|99287.12.peg.4389

Phylogenomic databases

eggNOGiENOG4105C12 Bacteria
COG0151 LUCA
HOGENOMiHOG000033463
KOiK01945
OMAiKATVCKY
PhylomeDBiP26977

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00125

BioCyciSENT99287:G1FZD-4215-MONOMER

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPUR2_SALTY
AccessioniPrimary (citable) accession number: P26977
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 10, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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