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UniProtKB - P26927 (HGFL_HUMAN)
Protein
Hepatocyte growth factor-like protein
Gene
MST1
Organism
Homo sapiens (Human)
Status
Functioni
Caution
Although related to peptidase S1 family, the active site residues characteristic of serine proteases appear to be absent from this protein, which may therefore lack protease activity.Curated
GO - Molecular functioni
- receptor tyrosine kinase binding Source: UniProtKB
- signaling receptor binding Source: GO_Central
GO - Biological processi
- histone H2A-S139 phosphorylation Source: CACAO
- negative regulation of gluconeogenesis Source: UniProtKB
- proteolysis Source: InterPro
- regulation of cAMP-dependent protein kinase activity Source: UniProtKB
- regulation of receptor signaling pathway via JAK-STAT Source: GO_Central
Keywordsi
Molecular function | Serine protease homolog |
Enzyme and pathway databases
PathwayCommonsi | P26927 |
Reactomei | R-HSA-8852405, Signaling by MST1 |
SignaLinki | P26927 |
SIGNORi | P26927 |
Protein family/group databases
MEROPSi | S01.975 |
Names & Taxonomyi
Protein namesi | Recommended name: Hepatocyte growth factor-like proteinAlternative name(s): Macrophage stimulatory protein Macrophage-stimulating protein Short name: MSP Cleaved into the following 2 chains: |
Gene namesi | Name:MST1 Synonyms:D3F15S2, DNF15S2, HGFL |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7380, MST1 |
MIMi | 142408, gene |
neXtProti | NX_P26927 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Other locations
- collagen-containing extracellular matrix Source: BHF-UCL
Keywords - Cellular componenti
SecretedPathology & Biotechi
Involvement in diseasei
MST1 variant Cys-689 may be associated with inflammatory bowel disease (IBD), a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is unsure whether Cys-689 itself or a variation in linkage disequilibrium with Cys-689 is responsible for the association with IBD.2 Publications
Organism-specific databases
DisGeNETi | 4485 |
MalaCardsi | MST1 |
Orphaneti | 171, Primary sclerosing cholangitis |
PharmGKBi | PA31185 |
Miscellaneous databases
Pharosi | P26927, Tchem |
Chemistry databases
ChEMBLi | CHEMBL6042 |
Genetic variation databases
BioMutai | MST1 |
DMDMi | 147744563 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000028085 | 19 – 711 | Hepatocyte growth factor-like proteinAdd BLAST | 693 | |
ChainiPRO_0000028086 | 19 – 483 | Hepatocyte growth factor-like protein alpha chainCuratedAdd BLAST | 465 | |
ChainiPRO_0000028087 | 484 – 711 | Hepatocyte growth factor-like protein beta chainCuratedAdd BLAST | 228 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 56 ↔ 78 | By similarity | ||
Disulfide bondi | 60 ↔ 66 | By similarity | ||
Glycosylationi | 72 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 110 ↔ 186 | By similarity | ||
Disulfide bondi | 131 ↔ 169 | By similarity | ||
Disulfide bondi | 157 ↔ 181 | By similarity | ||
Disulfide bondi | 191 ↔ 268 | By similarity | ||
Disulfide bondi | 194 ↔ 324 | By similarity | ||
Disulfide bondi | 212 ↔ 251 | By similarity | ||
Disulfide bondi | 240 ↔ 263 | By similarity | ||
Disulfide bondi | 283 ↔ 361 | By similarity | ||
Glycosylationi | 296 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 304 ↔ 343 | By similarity | ||
Disulfide bondi | 332 ↔ 355 | By similarity | ||
Disulfide bondi | 370 ↔ 448 | By similarity | ||
Disulfide bondi | 391 ↔ 431 | By similarity | ||
Disulfide bondi | 419 ↔ 443 | By similarity | ||
Disulfide bondi | 468 ↔ 588 | Interchain (between alpha and beta chains) | ||
Disulfide bondi | 507 ↔ 523 | By similarity | ||
Disulfide bondi | 602 ↔ 667 | By similarity | ||
Glycosylationi | 615 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 632 ↔ 646 | By similarity | ||
Disulfide bondi | 657 ↔ 685 | By similarity |
Post-translational modificationi
Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains.
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
CPTACi | non-CPTAC-2672 |
jPOSTi | P26927 |
MassIVEi | P26927 |
MaxQBi | P26927 |
PaxDbi | P26927 |
PeptideAtlasi | P26927 |
PRIDEi | P26927 |
ProteomicsDBi | 54368 |
PTM databases
GlyConnecti | 1310, 5 N-Linked glycans (5 sites) |
GlyGeni | P26927, 6 sites, 6 N-linked glycans (5 sites), 1 O-linked glycan (1 site) |
iPTMneti | P26927 |
PhosphoSitePlusi | P26927 |
Interactioni
Subunit structurei
Dimer of an alpha chain and a beta chain linked by a disulfide bond.
Interacts (via beta chain) with MST1R (via SEMA domain).
3 PublicationsBinary interactionsi
P26927
With | #Exp. | IntAct |
---|---|---|
MST1R [Q04912] | 5 | EBI-6929133,EBI-2637518 |
SFT2D1 [Q8WV19] | 3 | EBI-6929133,EBI-2854842 |
STK11 [Q15831] | 2 | EBI-6929133,EBI-306838 |
GO - Molecular functioni
- receptor tyrosine kinase binding Source: UniProtKB
- signaling receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 110591, 15 interactors |
DIPi | DIP-6028N |
IntActi | P26927, 9 interactors |
MINTi | P26927 |
STRINGi | 9606.ENSP00000414287 |
Chemistry databases
BindingDBi | P26927 |
Miscellaneous databases
RNActi | P26927, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P26927 |
SMRi | P26927 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26927 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 105 | PANPROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 110 – 186 | Kringle 1PROSITE-ProRule annotationAdd BLAST | 77 | |
Domaini | 191 – 268 | Kringle 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 283 – 361 | Kringle 3PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 370 – 448 | Kringle 4PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 484 – 709 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 226 |
Sequence similaritiesi
Keywords - Domaini
Kringle, Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QRJ0, Eukaryota |
InParanoidi | P26927 |
OrthoDBi | 164039at2759 |
PhylomeDBi | P26927 |
Family and domain databases
CDDi | cd00108, KR, 4 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 4 hits |
InterProi | View protein in InterPro IPR024174, HGF/MST1 IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR043575, MSP_HGFL IPR003609, Pan_app IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00051, Kringle, 4 hits PF00024, PAN_1, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001152, HGF_MST1, 1 hit PIRSF500185, MSP_HGFL, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 4 hits SM00473, PAN_AP, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 4 hits |
PROSITEi | View protein in PROSITE PS00021, KRINGLE_1, 4 hits PS50070, KRINGLE_2, 4 hits PS50948, PAN, 1 hit PS50240, TRYPSIN_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P26927-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGWLPLLLLL TQCLGVPGQR SPLNDFQVLR GTELQHLLHA VVPGPWQEDV
60 70 80 90 100
ADAEECAGRC GPLMDCRAFH YNVSSHGCQL LPWTQHSPHT RLRRSGRCDL
110 120 130 140 150
FQKKDYVRTC IMNNGVGYRG TMATTVGGLP CQAWSHKFPN DHKYTPTLRN
160 170 180 190 200
GLEENFCRNP DGDPGGPWCY TTDPAVRFQS CGIKSCREAA CVWCNGEEYR
210 220 230 240 250
GAVDRTESGR ECQRWDLQHP HQHPFEPGKF LDQGLDDNYC RNPDGSERPW
260 270 280 290 300
CYTTDPQIER EFCDLPRCGS EAQPRQEATT VSCFRGKGEG YRGTANTTTA
310 320 330 340 350
GVPCQRWDAQ IPHQHRFTPE KYACKDLREN FCRNPDGSEA PWCFTLRPGM
360 370 380 390 400
RAAFCYQIRR CTDDVRPQDC YHGAGEQYRG TVSKTRKGVQ CQRWSAETPH
410 420 430 440 450
KPQFTFTSEP HAQLEENFCR NPDGDSHGPW CYTMDPRTPF DYCALRRCAD
460 470 480 490 500
DQPPSILDPP DQVQFEKCGK RVDRLDQRRS KLRVVGGHPG NSPWTVSLRN
510 520 530 540 550
RQGQHFCGGS LVKEQWILTA RQCFSSCHMP LTGYEVWLGT LFQNPQHGEP
560 570 580 590 600
SLQRVPVAKM VCGPSGSQLV LLKLERSVTL NQRVALICLP PEWYVVPPGT
610 620 630 640 650
KCEIAGWGET KGTGNDTVLN VALLNVISNQ ECNIKHRGRV RESEMCTEGL
660 670 680 690 700
LAPVGACEGD YGGPLACFTH NCWVLEGIII PNRVCARSRW PAVFTRVSVF
710
VDWIHKVMRL G
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3XAK1 | G3XAK1_HUMAN | Hepatocyte growth factor-like prote... | MST1 hCG_2001992 | 725 | Annotation score: | ||
H7C0F8 | H7C0F8_HUMAN | Hepatocyte growth factor-like prote... | MST1 | 195 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 292 | R → G AA sequence (PubMed:1827141).Curated | 1 | |
Sequence conflicti | 304 | C → E AA sequence (PubMed:1827141).Curated | 1 | |
Sequence conflicti | 306 | R → E AA sequence (PubMed:1827141).Curated | 1 | |
Sequence conflicti | 550 | Missing AA sequence (PubMed:1827141).Curated | 1 | |
Sequence conflicti | 593 | W → E AA sequence (PubMed:1827141).Curated | 1 | |
Sequence conflicti | 623 | L → F in AAA59872 (PubMed:8393443).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_006631 | 13 | C → Y1 Publication | 1 | |
Natural variantiVAR_006632 | 212 | C → F. | 1 | |
Natural variantiVAR_059787 | 551 | S → G. Corresponds to variant dbSNP:rs6791037Ensembl. | 1 | |
Natural variantiVAR_070224 | 689 | R → C May be associated with inflammatory bowel disease; results in reduced binding affinity to MST1R. 3 PublicationsCorresponds to variant dbSNP:rs3197999Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74178 mRNA Translation: AAA50165.1 U37055 Genomic DNA Translation: AAC50471.1 L11924 mRNA Translation: AAA59872.1 AC099668 Genomic DNA No translation available. BC048330 mRNA Translation: AAH48330.1 |
PIRi | A40331, A47136 |
RefSeqi | NP_066278.3, NM_020998.3 |
Genome annotation databases
GeneIDi | 4485 |
KEGGi | hsa:4485 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74178 mRNA Translation: AAA50165.1 U37055 Genomic DNA Translation: AAC50471.1 L11924 mRNA Translation: AAA59872.1 AC099668 Genomic DNA No translation available. BC048330 mRNA Translation: AAH48330.1 |
PIRi | A40331, A47136 |
RefSeqi | NP_066278.3, NM_020998.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ASU | X-ray | 1.85 | A | 465-483 | [»] | |
B | 484-711 | [»] | ||||
4QT8 | X-ray | 3.00 | C/D | 465-711 | [»] | |
AlphaFoldDBi | P26927 | |||||
SMRi | P26927 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110591, 15 interactors |
DIPi | DIP-6028N |
IntActi | P26927, 9 interactors |
MINTi | P26927 |
STRINGi | 9606.ENSP00000414287 |
Chemistry databases
BindingDBi | P26927 |
ChEMBLi | CHEMBL6042 |
Protein family/group databases
MEROPSi | S01.975 |
PTM databases
GlyConnecti | 1310, 5 N-Linked glycans (5 sites) |
GlyGeni | P26927, 6 sites, 6 N-linked glycans (5 sites), 1 O-linked glycan (1 site) |
iPTMneti | P26927 |
PhosphoSitePlusi | P26927 |
Genetic variation databases
BioMutai | MST1 |
DMDMi | 147744563 |
Proteomic databases
CPTACi | non-CPTAC-2672 |
jPOSTi | P26927 |
MassIVEi | P26927 |
MaxQBi | P26927 |
PaxDbi | P26927 |
PeptideAtlasi | P26927 |
PRIDEi | P26927 |
ProteomicsDBi | 54368 |
Protocols and materials databases
CPTCi | P26927, 1 antibody |
DNASUi | 4485 |
Genome annotation databases
GeneIDi | 4485 |
KEGGi | hsa:4485 |
Organism-specific databases
CTDi | 4485 |
DisGeNETi | 4485 |
GeneCardsi | MST1 |
HGNCi | HGNC:7380, MST1 |
MalaCardsi | MST1 |
MIMi | 142408, gene |
neXtProti | NX_P26927 |
Orphaneti | 171, Primary sclerosing cholangitis |
PharmGKBi | PA31185 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QRJ0, Eukaryota |
InParanoidi | P26927 |
OrthoDBi | 164039at2759 |
PhylomeDBi | P26927 |
Enzyme and pathway databases
PathwayCommonsi | P26927 |
Reactomei | R-HSA-8852405, Signaling by MST1 |
SignaLinki | P26927 |
SIGNORi | P26927 |
Miscellaneous databases
BioGRID-ORCSi | 4485, 301 hits in 1034 CRISPR screens |
ChiTaRSi | MST1, human |
EvolutionaryTracei | P26927 |
GeneWikii | MST1 |
GenomeRNAii | 4485 |
Pharosi | P26927, Tchem |
PROi | PR:P26927 |
RNActi | P26927, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00108, KR, 4 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 2.40.20.10, 4 hits |
InterProi | View protein in InterPro IPR024174, HGF/MST1 IPR000001, Kringle IPR013806, Kringle-like IPR018056, Kringle_CS IPR038178, Kringle_sf IPR043575, MSP_HGFL IPR003609, Pan_app IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00051, Kringle, 4 hits PF00024, PAN_1, 1 hit PF00089, Trypsin, 1 hit |
PIRSFi | PIRSF001152, HGF_MST1, 1 hit PIRSF500185, MSP_HGFL, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00130, KR, 4 hits SM00473, PAN_AP, 1 hit SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF57440, SSF57440, 4 hits |
PROSITEi | View protein in PROSITE PS00021, KRINGLE_1, 4 hits PS50070, KRINGLE_2, 4 hits PS50948, PAN, 1 hit PS50240, TRYPSIN_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HGFL_HUMAN | |
Accessioni | P26927Primary (citable) accession number: P26927 Secondary accession number(s): A6NLA3 Q6GTN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | May 15, 2007 | |
Last modified: | May 25, 2022 | |
This is version 203 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families