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UniProtKB - P26811 (DPOL1_SACS2)
Protein
DNA polymerase 1
Gene
dpo1
Organism
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus)
Status
Functioni
This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. DNA polymerase I, DNA ligase and the flap endonuclease may be constitutively associated with the PCNA heterotrimer forming a scanning complex able to couple DNA synthesis and Okazaki fragment maturation.
1 PublicationCatalytic activityi
- EC:2.7.7.7
Activity regulationi
DNA synthesis is stimulated by PCNA heterotrimers.1 Publication
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: GO_Central
- exonuclease activity Source: UniProtKB-KW
- nucleotide binding Source: InterPro
GO - Biological processi
- DNA-dependent DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 3.1.11.1, 6163 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dpo1 Synonyms:polS Ordered Locus Names:SSO0552 |
Organismi | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) |
Taxonomic identifieri | 273057 [NCBI] |
Taxonomic lineagei | Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Saccharolobus › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 9 | Missing : No interaction with PCNA subunit PCNA2, DNA polymerase no longer stimulated by PCNA heterotrimers. 1 Publication | 9 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046483 | 1 – 882 | DNA polymerase 1Add BLAST | 882 |
Proteomic databases
PRIDEi | P26811 |
Interactioni
Subunit structurei
Interacts with PCNA subunit PCNA2 and weakly with PCNA3.
1 PublicationBinary interactionsi
P26811
With | #Exp. | IntAct |
---|---|---|
cdc6-1 [Q980N4] | 4 | EBI-15778666,EBI-9026921 |
cdc6-2 [Q9UXF8] | 5 | EBI-15778666,EBI-15778726 |
cdc6-3 [Q97WM8] | 3 | EBI-15778666,EBI-9026919 |
Protein-protein interaction databases
DIPi | DIP-48850N |
IntActi | P26811, 5 interactors |
STRINGi | 273057.SSO0552 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P26811 |
SMRi | P26811 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P26811 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | DisorderedSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the DNA polymerase type-B family.Curated
Phylogenomic databases
eggNOGi | arCOG15272, Archaea |
HOGENOMi | CLU_000203_6_0_2 |
InParanoidi | P26811 |
OMAi | WIRNMLY |
PhylomeDBi | P26811 |
Family and domain databases
Gene3Di | 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
i Sequence
Sequence statusi: Complete.
P26811-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKQLTLFDI PSSKPAKSEQ NTQQSQQSAP VEEKKVVRRE WLEEAQENKI
60 70 80 90 100
YFLLQVDYDG KKGKAVCKLF DKETQKIYAL YDNTGHKPYF LVDLEPDKVG
110 120 130 140 150
KIPKIVRDPS FDHIETVSKI DPYTWNKFKL TKIVVRDPLA VRRLRNDVPK
160 170 180 190 200
AYEAHIKYFN NYMYDIGLIP GMPYVVKNGK LESVYLSLDE KDVEEIKKAF
210 220 230 240 250
ADSDEMTRQM AVDWLPIFET EIPKIKRVAI DIEVYTPVKG RIPDSQKAEF
260 270 280 290 300
PIISIALAGS DGLKKVLVLN RNDVNEGSVK LDGISVERFN TEYELLGRFF
310 320 330 340 350
DILLEYPIVL TFNGDDFDLP YIYFRALKLG YFPEEIPIDV AGKDEAKYLA
360 370 380 390 400
GLHIDLYKFF FNKAVRNYAF EGKYNEYNLD AVAKALLGTS KVKVDTLISF
410 420 430 440 450
LDVEKLIEYN FRDAEITLQL TTFNNDLTMK LIVLFSRISR LGIEELTRTE
460 470 480 490 500
ISTWVKNLYY WEHRKRNWLI PLKEEILAKS SNIRTSALIK GKGYKGAVVI
510 520 530 540 550
DPPAGIFFNI TVLDFASLYP SIIRTWNLSY ETVDIQQCKK PYEVKDETGE
560 570 580 590 600
VLHIVCMDRP GITAVITGLL RDFRVKIYKK KAKNPNNSEE QKLLYDVVQR
610 620 630 640 650
AMKVFINATY GVFGAETFPL YAPAVAESVT ALGRYVITST VKKAREEGLT
660 670 680 690 700
VLYGDTDSLF LLNPPKNSLE NIIKWVKTTF NLDLEVDKTY KFVAFSGLKK
710 720 730 740 750
NYFGVYQDGK VDIKGMLVKK RNTPEFVKKV FNEVKELMIS INSPNDVKEI
760 770 780 790 800
KRKIVDVVKG SYEKLKNKGY NLDELAFKVM LSKPLDAYKK NTPQHVKAAL
810 820 830 840 850
QLRPFGVNVL PRDIIYYVKV RSKDGVKPVQ LAKVTEIDAE KYLEALRSTF
860 870 880
EQILRAFGVS WDEIAATMSI DSFFSYPSKG NS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 139 | L → H in CAA45795 (PubMed:1614858).Curated | 1 | |
Sequence conflicti | 624 | A → R in CAA45795 (PubMed:1614858).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64466 Genomic DNA Translation: CAA45795.1 U92875 Genomic DNA Translation: AAB53090.1 Y18930 Genomic DNA Translation: CAB57747.1 AE006641 Genomic DNA Translation: AAK40869.1 |
PIRi | F90201 S23019 |
RefSeqi | WP_009991059.1, NC_002754.1 |
Genome annotation databases
EnsemblBacteriai | AAK40869; AAK40869; SSO0552 |
GeneIDi | 44129558 |
KEGGi | sso:SSO0552 |
PATRICi | fig|273057.12.peg.562 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64466 Genomic DNA Translation: CAA45795.1 U92875 Genomic DNA Translation: AAB53090.1 Y18930 Genomic DNA Translation: CAB57747.1 AE006641 Genomic DNA Translation: AAK40869.1 |
PIRi | F90201 S23019 |
RefSeqi | WP_009991059.1, NC_002754.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1S5J | X-ray | 2.40 | A | 36-882 | [»] | |
AlphaFoldDBi | P26811 | |||||
SMRi | P26811 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48850N |
IntActi | P26811, 5 interactors |
STRINGi | 273057.SSO0552 |
Proteomic databases
PRIDEi | P26811 |
Genome annotation databases
EnsemblBacteriai | AAK40869; AAK40869; SSO0552 |
GeneIDi | 44129558 |
KEGGi | sso:SSO0552 |
PATRICi | fig|273057.12.peg.562 |
Phylogenomic databases
eggNOGi | arCOG15272, Archaea |
HOGENOMi | CLU_000203_6_0_2 |
InParanoidi | P26811 |
OMAi | WIRNMLY |
PhylomeDBi | P26811 |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6163 3.1.11.1, 6163 |
Miscellaneous databases
EvolutionaryTracei | P26811 |
Family and domain databases
Gene3Di | 3.30.420.10, 1 hit 3.90.1600.10, 1 hit |
InterProi | View protein in InterPro IPR006172, DNA-dir_DNA_pol_B IPR017964, DNA-dir_DNA_pol_B_CS IPR006133, DNA-dir_DNA_pol_B_exonuc IPR006134, DNA-dir_DNA_pol_B_multi_dom IPR043502, DNA/RNA_pol_sf IPR023211, DNA_pol_palm_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF00136, DNA_pol_B, 1 hit PF03104, DNA_pol_B_exo1, 1 hit |
PRINTSi | PR00106, DNAPOLB |
SMARTi | View protein in SMART SM00486, POLBc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00116, DNA_POLYMERASE_B, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOL1_SACS2 | |
Accessioni | P26811Primary (citable) accession number: P26811 Secondary accession number(s): O05707 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families