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Protein

Gag polyprotein

Gene

gag

Organism
Friend murine leukemia virus (isolate FB29) (FrMLV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Gag polyprotein: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.By similarity
Matrix protein p15: Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.By similarity
RNA-binding phosphoprotein p12: Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.By similarity
Capsid protein p30: Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10-Gag: Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Gene namesi
Name:gag
OrganismiFriend murine leukemia virus (isolate FB29) (FrMLV)
Taxonomic identifieri11797 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000008877 Componenti: Genome

Subcellular locationi

Gag polyprotein :
Matrix protein p15 :
Capsid protein p30 :
Nucleocapsid protein p10-Gag :
RNA-binding phosphoprotein p12 :

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host cytoplasm, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis
ChainiPRO_00003908122 – 538Gag polyproteinAdd BLAST537
ChainiPRO_00000409002 – 131Matrix protein p15Add BLAST130
ChainiPRO_0000040901132 – 215RNA-binding phosphoprotein p12Add BLAST84
ChainiPRO_0000040902216 – 478Capsid protein p30Add BLAST263
ChainiPRO_0000040903479 – 538Nucleocapsid protein p10-GagAdd BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostSequence analysis1
Modified residuei192Phosphoserine; by hostBy similarity1

Post-translational modificationi

Gag polyprotein: Ubiquitinated by ITCH. Gag can recruit the ubiquitin ligase Itch in an L domain-independent manner to facilitate virus release via a mechanism that involves Gag ubiquitination.By similarity
Gag polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation.By similarity
Capsid protein p30: Sumoylated; required for virus replication.By similarity
RNA-binding phosphoprotein p12 is phosphorylated on serine residues.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei131 – 132Cleavage; by viral proteaseBy similarity2
Sitei215 – 216Cleavage; by viral proteaseBy similarity2
Sitei478 – 479Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Capsid protein p30: Homohexamer; further associates as homomultimer (By similarity). Capsid protein p30: The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers. Capsid protein p30: Interacts with mouse UBE2I and mouse PIAS4. Gag polyprotein: Interacts (via PPXY motif) with host NEDD4. Gag polyprotein: Interacts (via PSAP motif) with host TSG101. Gag polyprotein: Interacts (via LYPX(n)L motif) with host PDCD6IP (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-61620N
ELMiP26806
IntActiP26806, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP26806
SMRiP26806
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 393Interaction with host PIAS4By similarityAdd BLAST49
Regioni430 – 435Interaction with host UBE2IBy similarity6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili438 – 478Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 114PTAP/PSAP motifBy similarity4
Motifi130 – 134LYPX(n)L motifBy similarity5
Motifi162 – 165PPXY motifBy similarity4

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP. Both PSAP and LYPX(n)L domains might play little to no role in budding and possibly drive residual virus release.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

OrthoDBiVOG090000NB

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Gag polyprotein (identifier: P26806-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGQAVTTPLS LTLDHWKDVE RTAHNLSVEV RKRRWVTFCS AEWPTFNVGW
60 70 80 90 100
PRDGTFNPDI ITQVKIKVFS PGPHGHPDQV PYIVTWEAIA VDPPPWVRPF
110 120 130 140 150
VHPKPPLSLP PSAPSLPPEP PLSTPPQSSL YPALTSPLNT KPRPQVLPDS
160 170 180 190 200
GGPLIDLLTE DPPPYRDPGP PSPDGNGDSG EVAPTEGAPD PSPMVSRLRG
210 220 230 240 250
RKEPPVADST TSQAFPLRLG GNGQYQYWPF SSSDLYNWKN NNPSFSEDPA
260 270 280 290 300
KLTALIESVL LTHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGEDGR
310 320 330 340 350
PTQLPNDIND AFPLERPDWD YNTQRGRNHL VHYRQLLLAG LQNAGRSPTN
360 370 380 390 400
LAKVKGITQG PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVAMSFIWQ
410 420 430 440 450
SAPDIGRKLE RLEDLKSKTL GDLVREAEKI FNKRETPEER EERIRRETEE
460 470 480 490 500
KEERRRAEDV QREKERDRRR HREMSKLLAT VVSGQRQDRQ GGERRRPQLD
510 520 530
HDQCAYCKEK GHWARDCPKK PRGPRGPRPQ ASLLTLDD
Length:538
Mass (Da):60,929
Last modified:January 23, 2007 - v3
Checksum:i2E652DDB9E0D4A3C
GO
Isoform Glyco-Gag protein (identifier: P0DOH5-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOH5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:626
Mass (Da):70,523
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501H → R AA sequence (PubMed:6267042).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11128 Genomic DNA Translation: CAA77478.1
PIRiS70394
RefSeqiNP_040332.1, NC_001362.1

Genome annotation databases

GeneIDi1491876
KEGGivg:1491876

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGAG_MLVFF
AccessioniPrimary (citable) accession number: P26806
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 122 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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