UniProtKB - P26785 (RL16B_YEAST)
60S ribosomal protein L16-B
RPL16B
Functioni
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.
1 PublicationMiscellaneous
GO - Molecular functioni
- mRNA binding Source: GO_Central
- RNA binding Source: SGD
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- cytoplasmic translation Source: SGD
- negative regulation of translation Source: GO_Central
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L16-B1 PublicationAlternative name(s): L21 Large ribosomal subunit protein uL13-B1 Publication RP23 YL15 |
Gene namesi | Ordered Locus Names:YNL069C ORF Names:N2377 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005013, RPL16B |
VEuPathDBi | FungiDB:YNL069C |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: Reactome
- cytosolic large ribosomal subunit Source: SGD
Other locations
- ribosome Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000133792 | 2 – 198 | 60S ribosomal protein L16-BAdd BLAST | 197 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine; partial2 Publications | 1 | |
Modified residuei | 43 | PhosphoserineCombined sources | 1 | |
Cross-linki | 176 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 185 | PhosphoserineCombined sources | 1 | |
Modified residuei | 187 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P26785 |
PaxDbi | P26785 |
PRIDEi | P26785 |
PTM databases
iPTMneti | P26785 |
Interactioni
Subunit structurei
Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).
1 Publication1 PublicationProtein-protein interaction databases
BioGRIDi | 35754, 151 interactors |
IntActi | P26785, 26 interactors |
MINTi | P26785 |
STRINGi | 4932.YNL069C |
Miscellaneous databases
RNActi | P26785, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
HOGENOMi | CLU_076922_0_0_1 |
InParanoidi | P26785 |
OMAi | WRTMRGM |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit PTHR11545:SF3, PTHR11545:SF3, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSQPVVVIDA KDHLLGRLAS TIAKQVLNGQ KIVVVRAEAL NISGEFFRNK
60 70 80 90 100
LKYHDFLRKA TAFNKTRGPF HFRAPSRILY KAIRGMVSHK TARGKAALER
110 120 130 140 150
LKIFEGIPPP YDKKKRVVVP QALRVLRLKP GRKYTTLGKL STSVGWKYED
160 170 180 190
VVAKLEDKRK VRSAEYYAKK RAFTKKVSSA SAAASESDVA KQLASFGY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | L → H AA sequence (PubMed:1544921).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86470 Genomic DNA Translation: CAA60191.1 X86470 Genomic DNA Translation: CAA60192.1 Z71345 Genomic DNA Translation: CAA95943.1 BK006947 Genomic DNA Translation: DAA10476.1 |
PIRi | S53911 |
RefSeqi | NP_014330.1, NM_001182907.1 |
Genome annotation databases
EnsemblFungii | YNL069C_mRNA; YNL069C; YNL069C |
GeneIDi | 855655 |
KEGGi | sce:YNL069C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X86470 Genomic DNA Translation: CAA60191.1 X86470 Genomic DNA Translation: CAA60192.1 Z71345 Genomic DNA Translation: CAA95943.1 BK006947 Genomic DNA Translation: DAA10476.1 |
PIRi | S53911 |
RefSeqi | NP_014330.1, NM_001182907.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4V88 | X-ray | 3.00 | BO/DO | 4-162 | [»] | |
BO/DO | 144-182 | [»] | ||||
6QIK | electron microscopy | 3.10 | J | 1-198 | [»] | |
6QT0 | electron microscopy | 3.40 | J | 1-198 | [»] | |
6QTZ | electron microscopy | 3.50 | J | 1-198 | [»] | |
6RI5 | electron microscopy | 3.30 | J | 1-198 | [»] | |
6RZZ | electron microscopy | 3.20 | J | 1-198 | [»] | |
6S05 | electron microscopy | 3.90 | J | 1-198 | [»] | |
AlphaFoldDBi | P26785 | |||||
SMRi | P26785 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35754, 151 interactors |
IntActi | P26785, 26 interactors |
MINTi | P26785 |
STRINGi | 4932.YNL069C |
PTM databases
iPTMneti | P26785 |
Proteomic databases
MaxQBi | P26785 |
PaxDbi | P26785 |
PRIDEi | P26785 |
Genome annotation databases
EnsemblFungii | YNL069C_mRNA; YNL069C; YNL069C |
GeneIDi | 855655 |
KEGGi | sce:YNL069C |
Organism-specific databases
SGDi | S000005013, RPL16B |
VEuPathDBi | FungiDB:YNL069C |
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
HOGENOMi | CLU_076922_0_0_1 |
InParanoidi | P26785 |
OMAi | WRTMRGM |
Miscellaneous databases
PROi | PR:P26785 |
RNActi | P26785, protein |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit PTHR11545:SF3, PTHR11545:SF3, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RL16B_YEAST | |
Accessioni | P26785Primary (citable) accession number: P26785 Secondary accession number(s): D6W1B0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 189 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - Ribosomal proteins
Ribosomal proteins families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families