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UniProtKB - P26760 (RTX1B_ACTPL)
Protein
Toxin RTX-I translocation ATP-binding protein
Gene
apxIB
Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Functioni
Involved in the transport of the toxin RTX-I as well as that of RTX-II.
Miscellaneous
The sequence shown is that of serotype 9.
In strain CM15 (serotype 1), disruption of this gene prevents production of both RTX-I and RTX-II toxin, and no hemolytic activity in mice or pigs.
Caution
Tyr-9 is present instead of the conserved Cys which is expected to be the active site residue of peptidase C39. Thus they are presumed to be without peptidase activity.Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 83 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 502 – 509 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type transporter activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- peptidase activity Source: InterPro
GO - Biological processi
- hemolysis in another organism Source: UniProtKB-KW
- protein secretion by the type I secretion system Source: InterPro
Keywordsi
Biological process | Cytolysis, Hemolysis, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Toxin RTX-I translocation ATP-binding proteinAlternative name(s): APX-IB Cytolysin IB Short name: CLY-IB HLY-IB RTX-I toxin determinant B |
Gene namesi | Name:apxIB Synonyms:appB, clyIB, hlyIB |
Organismi | Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae) |
Taxonomic identifieri | 715 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus |
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- type I protein secretion system complex Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 158 – 178 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 188 – 208 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 295 – 315 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 387 – 407 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 410 – 430 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000092390 | 1 – 707 | Toxin RTX-I translocation ATP-binding proteinAdd BLAST | 707 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
STRINGi | 228399.appser1_15230 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 125 | Peptidase C39PROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 154 – 436 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 468 – 703 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 236 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Protein-1 exporter (TC 3.A.1.109) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
i Sequence
Sequence statusi: Complete.
P26760-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFYREEDYG LYALTILAQY HNIAVNPEEL KHKFDLEGKG LDLTAWLLAA
60 70 80 90 100
KSLELKAKQV KKAIDRLAFI ALPALVWRED GKHFILTKID NEAKKYLIFD
110 120 130 140 150
LETHNPRILE QAEFESLYQG KLILVASRAS IVGKLAKFDF TWFIPAVIKY
160 170 180 190 200
RKIFIETLIV SIFLQIFALI TPLFFQVVMD KVLVHRGFST LNVITVALAI
210 220 230 240 250
VVLFEIVLNG LRTYIFAHST SRIDVELGAR LFRHLLALPI SYFENRRVGD
260 270 280 290 300
TVARVRELDQ IRNFLTGQAL TSVLDLMFSF IFFAVMWYYS PKLTLVILGS
310 320 330 340 350
LPFYMGWSIF ISPILRRRLD EKFARGADNQ SFLVESVTAI NTIKALAVTP
360 370 380 390 400
QMTNTWDKQL ASYVSAGFRV TTLATIGQQG VQFIQKVVMV ITLWLGAHLV
410 420 430 440 450
ISGDLSIGQL IAFNMLSGQV IAPVIRLAQL WQDFQQVGIS VTRLGDVLNS
460 470 480 490 500
PTESYQGKLA LPEIKGDITF RNIRFRYKPD APVILNDVNL SIQQGEVIGI
510 520 530 540 550
VGRSGSGKST LTKLIQRFYI PENGQVLIDG HDLALADPNW LRRQVGVVLQ
560 570 580 590 600
DNVLLNRSIR DNIALADPGM PMEKIVHAAK LAGAHEFISE LREGYNTIVG
610 620 630 640 650
EQGAGLSGGQ RQRIAIARAL VNNPKILIFD EATSALDYES EHIIMRNMHQ
660 670 680 690 700
ICKGRTVIII AHRLSTVKNA DRIIVMEKGQ IVEQGKHKEL LADPNGLYHY
LHQLQSE
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 110 – 112 | EQA → KQT in strain: Serotype 5. | 3 | |
Natural varianti | 328 | D → H in strain: Serotype 5. | 1 | |
Natural varianti | 517 | R → V in strain: Serotype 5. | 1 | |
Natural varianti | 556 | N → G in strain: Serotype 5. | 1 | |
Natural varianti | 609 – 611 | GQR → RAT in strain: S 4074 / Serotype 1. | 3 | |
Natural varianti | 611 – 612 | RQ → PN in strain: Serotype 5. | 2 | |
Natural varianti | 671 – 672 | DR → AS in strain: Serotype 5. | 2 | |
Natural varianti | 692 | A → R in strain: Serotype 5. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65808 Genomic DNA Translation: AAB00966.1 X61112 Genomic DNA Translation: CAA43425.1 X68595 Genomic DNA Translation: CAA48587.1 |
PIRi | D43599 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M65808 Genomic DNA Translation: AAB00966.1 X61112 Genomic DNA Translation: CAA43425.1 X68595 Genomic DNA Translation: CAA48587.1 |
PIRi | D43599 |
3D structure databases
AlphaFoldDBi | P26760 |
SMRi | P26760 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 228399.appser1_15230 |
Family and domain databases
CDDi | cd02417, Peptidase_C39_likeA, 1 hit |
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR010132, ATPase_T1SS_HlyB IPR027417, P-loop_NTPase IPR005074, Peptidase_C39 IPR039395, Peptidase_C39-like_A IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF03412, Peptidase_C39, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF90123, SSF90123, 1 hit |
TIGRFAMsi | TIGR01846, type_I_sec_HlyB, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS50990, PEPTIDASE_C39, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RTX1B_ACTPL | |
Accessioni | P26760Primary (citable) accession number: P26760 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | November 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |