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Protein

Terminase, large subunit

Gene

2

Organism
Salmonella phage P22 (Bacteriophage P22)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by neck proteins.By similarity

Cofactori

Mg2+1 PublicationNote: Binds 2 Mg2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei204For ATPase activitySequence analysis1
Metal bindingi321Magnesium 1; catalytic; for nuclease activity1 Publication1
Metal bindingi326Magnesium 2; catalytic; for nuclease activity1 Publication1
Metal bindingi459Magnesium 1; catalytic; for nuclease activity1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 102ATPSequence analysis8

GO - Molecular functioni

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processViral genome packaging, Viral release from host cell
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Terminase, large subunit (EC:3.1.-.-1 Publication)
Alternative name(s):
DNA-packaging protein gp2
Gene product 2
Short name:
gp2
Gene namesi
Name:2
OrganismiSalmonella phage P22 (Bacteriophage P22)
Taxonomic identifieri10754 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeP22virus
Virus hostiSalmonella typhimurium [TaxID: 90371]
Proteomesi
  • UP000001795 Componenti: Genome
  • UP000007960 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • viral terminase, large subunit Source: UniProtKB
  • viral terminase complex Source: UniProtKB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000777491 – 499Terminase, large subunitAdd BLAST499

Proteomic databases

PRIDEiP26745

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with the terminase small subunit gp3; the active complex is probably heterooligomeric (PubMed:22715098). Interacts with the portal protein; this interaction allows the packaging of viral DNA (PubMed:28134243).1 Publication2 Publications

Protein-protein interaction databases

DIPiDIP-59856N
IntActiP26745, 1 interactor

Structurei

Secondary structure

1499
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SMRiP26745
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 286ATPase activity1 PublicationAdd BLAST286
Regioni1 – 58Interaction with the terminase small subunit gp31 PublicationAdd BLAST58
Regioni312 – 499Nuclease activity1 PublicationAdd BLAST188

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 67Walker A motif8
Motifi199 – 204Walker B motif6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 26Poly-GluSequence analysis4

Sequence similaritiesi

Belongs to the T4likevirus large terminase family.Curated

Phylogenomic databases

OrthoDBiVOG0900006F

Family and domain databases

InterProiView protein in InterPro
IPR035421 Terminase_6C
IPR004921 Terminase_lsu_T4like
PfamiView protein in Pfam
PF03237 Terminase_6, 1 hit
PF17289 Terminase_6C, 1 hit

Sequencei

Sequence statusi: Complete.

P26745-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELDAILDNL SDEEQIELLE LLEEEENYRN THLLYEFAPY SKQREFIDAG
60 70 80 90 100
HDYPERCFMA GNQLGKSFTG AAEVAFHLTG RYPGTKGYPA DGKYGGEWKG
110 120 130 140 150
KRFYEPVVFW IGGETNETVT KTTQRILCGR IEENDEPGYG SIPKEDIISW
160 170 180 190 200
KKSPFFPNLV DHLLVKHHTA DGVEDGISIC YFKPYSQGRA RWQGDTIHGV
210 220 230 240 250
WFDEEPPYSI YGEGLTRTNK YGQFSILTFT PLMGMSDVVT KFLKNPSKSQ
260 270 280 290 300
KVVNMTIYDA EHYTDEQKEQ IIASYPEHER EARARGIPTM GSGRIFQIPE
310 320 330 340 350
ETIKCQPFEC PDHFYVIDAQ DFGWNHPQAH IQLWWDKDAD VFYLARVWKK
360 370 380 390 400
SENTAVQAWG AVKSWANKIP VAWPHDGHQH EKGGGEQLKT QYADAGFSML
410 420 430 440 450
PDHATFPDGG NSVESGISEL RDLMLEGRFK VFNTCEPFFE EFRLYHRDEN
460 470 480 490
GKIVKTNDDV LDATRYGYMM RRFARMMRDI RKPKEKKIPA PIRPVRRGR
Length:499
Mass (Da):57,592
Last modified:August 1, 1992 - v1
Checksum:i6E8FE06F43182C39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59749 Genomic DNA Translation: AAA72959.1
AF217253 Genomic DNA Translation: AAF75044.1
BK000583 Genomic DNA Translation: DAA00977.1
PIRiB40474 Z2BP22
RefSeqiYP_063734.1, NC_002371.2

Genome annotation databases

GeneIDi2944239
KEGGivg:2944239

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59749 Genomic DNA Translation: AAA72959.1
AF217253 Genomic DNA Translation: AAF75044.1
BK000583 Genomic DNA Translation: DAA00977.1
PIRiB40474 Z2BP22
RefSeqiYP_063734.1, NC_002371.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DKWX-ray2.02A/B/C/D289-499[»]
SMRiP26745
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59856N
IntActiP26745, 1 interactor

Proteomic databases

PRIDEiP26745

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2944239
KEGGivg:2944239

Phylogenomic databases

OrthoDBiVOG0900006F

Family and domain databases

InterProiView protein in InterPro
IPR035421 Terminase_6C
IPR004921 Terminase_lsu_T4like
PfamiView protein in Pfam
PF03237 Terminase_6, 1 hit
PF17289 Terminase_6C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTERL_BPP22
AccessioniPrimary (citable) accession number: P26745
Secondary accession number(s): Q7PCJ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 7, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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