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Protein

Twist-related protein 1

Gene

Twist1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer.5 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Repressor
Biological processBiological rhythms, Differentiation, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Twist-related protein 1
Alternative name(s):
M-twist
Gene namesi
Name:Twist1
Synonyms:Twist
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:98872 Twist1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi179C → G: High transactivation activity. 1 Publication1
Mutagenesisi185E → D, K or R: High transactivation activity. 1 Publication1
Mutagenesisi186R → A or K: High transactivation activity. 1 Publication1
Mutagenesisi187L → G: Low transactivation activity. 1 Publication1
Mutagenesisi188S → A: High transactivation activity. 1 Publication1
Mutagenesisi191F → A, G or P: Low transactivation activity. 1 Publication1
Mutagenesisi192S → A: High transactivation activity. 1 Publication1
Mutagenesisi192S → P: Intermediate transactivation activity. 1 Publication1
Mutagenesisi195R → E: Intermediate transactivation activity. 1 Publication1
Mutagenesisi195R → G: Low transactivation activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274881 – 206Twist-related protein 1Add BLAST206

Proteomic databases

PaxDbiP26687
PRIDEiP26687

PTM databases

iPTMnetiP26687
PhosphoSitePlusiP26687

Miscellaneous databases

PMAP-CutDBiP26687

Expressioni

Tissue specificityi

Subset of mesodermal cells.1 Publication

Inductioni

By TNF-alpha.1 Publication

Gene expression databases

BgeeiENSMUSG00000035799 Expressed in 269 organ(s), highest expression level in maxillary prominence
CleanExiMM_TWIST1
GenevisibleiP26687 MM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204385, 6 interactors
DIPiDIP-61633N
IntActiP26687, 6 interactors
STRINGi10090.ENSMUSP00000040089

Structurei

3D structure databases

ProteinModelPortaliP26687
SMRiP26687
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini112 – 163bHLHPROSITE-ProRule annotationAdd BLAST52

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 195Sufficient for transactivation activityAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi80 – 102Gly-richAdd BLAST23

Phylogenomic databases

eggNOGiKOG4447 Eukaryota
ENOG4111QFU LUCA
GeneTreeiENSGT00760000119097
HOGENOMiHOG000261629
InParanoidiP26687
KOiK09069
OMAiDRQPKRC
OrthoDBiEOG091G07PB
TreeFamiTF315153

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR015789 Twist-related
PANTHERiPTHR23349:SF64 PTHR23349:SF64, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

P26687-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMQDVSSSPV SPADDSLSNS EEEPDRQQPA SGKRGARKRR SSRRSAGGSA
60 70 80 90 100
GPGGATGGGI GGGDEPGSPA QGKRGKKSAG GGGGGGAGGG GGGGGGSSSG
110 120 130 140 150
GGSPQSYEEL QTQRVMANVR ERQRTQSLNE AFAALRKIIP TLPSDKLSKI
160 170 180 190 200
QTLKLAARYI DFLYQVLQSD ELDSKMASCS YVAHERLSYA FSVWRMEGAW

SMSASH
Length:206
Mass (Da):21,198
Last modified:August 1, 1992 - v1
Checksum:i618AD8E9BE87C555
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36A → R in AAA40515 (PubMed:1840517).Curated1
Sequence conflicti91G → P in AAA40515 (PubMed:1840517).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63649 Genomic DNA Translation: AAA40514.1
M63650 mRNA Translation: AAA40515.1
BC033434 mRNA Translation: AAH33434.1
BC083139 mRNA Translation: AAH83139.1
CCDSiCCDS25879.1
PIRiI53066
RefSeqiNP_035788.1, NM_011658.2
UniGeneiMm.3280

Genome annotation databases

EnsembliENSMUST00000049089; ENSMUSP00000040089; ENSMUSG00000035799
GeneIDi22160
KEGGimmu:22160
UCSCiuc007niw.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63649 Genomic DNA Translation: AAA40514.1
M63650 mRNA Translation: AAA40515.1
BC033434 mRNA Translation: AAH33434.1
BC083139 mRNA Translation: AAH83139.1
CCDSiCCDS25879.1
PIRiI53066
RefSeqiNP_035788.1, NM_011658.2
UniGeneiMm.3280

3D structure databases

ProteinModelPortaliP26687
SMRiP26687
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204385, 6 interactors
DIPiDIP-61633N
IntActiP26687, 6 interactors
STRINGi10090.ENSMUSP00000040089

PTM databases

iPTMnetiP26687
PhosphoSitePlusiP26687

Proteomic databases

PaxDbiP26687
PRIDEiP26687

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049089; ENSMUSP00000040089; ENSMUSG00000035799
GeneIDi22160
KEGGimmu:22160
UCSCiuc007niw.1 mouse

Organism-specific databases

CTDi7291
MGIiMGI:98872 Twist1

Phylogenomic databases

eggNOGiKOG4447 Eukaryota
ENOG4111QFU LUCA
GeneTreeiENSGT00760000119097
HOGENOMiHOG000261629
InParanoidiP26687
KOiK09069
OMAiDRQPKRC
OrthoDBiEOG091G07PB
TreeFamiTF315153

Miscellaneous databases

PMAP-CutDBiP26687
PROiPR:P26687
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035799 Expressed in 269 organ(s), highest expression level in maxillary prominence
CleanExiMM_TWIST1
GenevisibleiP26687 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR015789 Twist-related
PANTHERiPTHR23349:SF64 PTHR23349:SF64, 1 hit
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTWST1_MOUSE
AccessioniPrimary (citable) accession number: P26687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 12, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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