UniProtKB - P26687 (TWST1_MOUSE)
Protein
Twist-related protein 1
Gene
Twist1
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer.5 Publications
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- E-box binding Source: UniProtKB
- identical protein binding Source: MGI
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
- transcription factor binding Source: BHF-UCL
GO - Biological processi
- aortic valve morphogenesis Source: BHF-UCL
- cardiac neural crest cell development involved in outflow tract morphogenesis Source: MGI
- cardiac neural crest cell migration involved in outflow tract morphogenesis Source: MGI
- cell differentiation Source: MGI
- cell proliferation involved in heart valve development Source: BHF-UCL
- cellular response to growth factor stimulus Source: Ensembl
- cellular response to hypoxia Source: BHF-UCL
- cranial suture morphogenesis Source: UniProtKB
- developmental process Source: GO_Central
- embryonic camera-type eye formation Source: MGI
- embryonic cranial skeleton morphogenesis Source: BHF-UCL
- embryonic digit morphogenesis Source: MGI
- embryonic forelimb morphogenesis Source: MGI
- embryonic hindlimb morphogenesis Source: BHF-UCL
- embryonic limb morphogenesis Source: MGI
- embryonic skeletal system morphogenesis Source: MGI
- endocardial cushion morphogenesis Source: MGI
- eyelid development in camera-type eye Source: MGI
- hindlimb morphogenesis Source: MGI
- in utero embryonic development Source: MGI
- mitral valve morphogenesis Source: BHF-UCL
- muscle organ development Source: UniProtKB-KW
- negative regulation of apoptotic process Source: MGI
- negative regulation of cell differentiation Source: UniProtKB
- negative regulation of cellular senescence Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
- negative regulation of DNA damage response, signal transduction by p53 class mediator Source: MGI
- negative regulation of double-strand break repair Source: MGI
- negative regulation of histone acetylation Source: BHF-UCL
- negative regulation of histone phosphorylation Source: MGI
- negative regulation of molecular function Source: MGI
- negative regulation of osteoblast differentiation Source: MGI
- negative regulation of oxidative phosphorylation uncoupler activity Source: BHF-UCL
- negative regulation of peroxisome proliferator activated receptor signaling pathway Source: BHF-UCL
- negative regulation of phosphatidylinositol 3-kinase signaling Source: MGI
- negative regulation of skeletal muscle tissue development Source: MGI
- negative regulation of striated muscle tissue development Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- negative regulation of tumor necrosis factor production Source: MGI
- neural tube closure Source: MGI
- neuron migration Source: MGI
- odontogenesis Source: Ensembl
- ossification Source: MGI
- osteoblast differentiation Source: MGI
- positive regulation of cell motility Source: BHF-UCL
- positive regulation of DNA-templated transcription, initiation Source: MGI
- positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation Source: BHF-UCL
- positive regulation of epithelial cell proliferation Source: MGI
- positive regulation of epithelial to mesenchymal transition Source: BHF-UCL
- positive regulation of fatty acid beta-oxidation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of interleukin-6 production Source: MGI
- positive regulation of monocyte chemotactic protein-1 production Source: MGI
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transcription regulatory region DNA binding Source: MGI
- positive regulation of tumor necrosis factor production Source: MGI
- regulation of bone mineralization Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- rhythmic process Source: UniProtKB-KW
- roof of mouth development Source: Ensembl
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Biological rhythms, Differentiation, Myogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Twist-related protein 1Alternative name(s): M-twist |
Gene namesi | Name:Twist1 Synonyms:Twist |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98872, Twist1 |
Subcellular locationi
Nucleus
Nucleus
- nuclear chromatin Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 179 | C → G: High transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 185 | E → D, K or R: High transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 186 | R → A or K: High transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 187 | L → G: Low transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 188 | S → A: High transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 191 | F → A, G or P: Low transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 192 | S → A: High transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 192 | S → P: Intermediate transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 195 | R → E: Intermediate transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 195 | R → G: Low transactivation activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127488 | 1 – 206 | Twist-related protein 1Add BLAST | 206 |
Proteomic databases
jPOSTi | P26687 |
PaxDbi | P26687 |
PRIDEi | P26687 |
PTM databases
iPTMneti | P26687 |
PhosphoSitePlusi | P26687 |
Expressioni
Tissue specificityi
Subset of mesodermal cells.1 Publication
Inductioni
By TNF-alpha.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000035799, Expressed in maxillary prominence and 286 other tissues |
Genevisiblei | P26687, MM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed.
2 PublicationsBinary interactionsi
Hide detailsP26687
With | #Exp. | IntAct |
---|---|---|
G3BP2 [Q9UN86] from Homo sapiens. | 2 | EBI-6123119,EBI-1044298 |
TP53 [P04637] from Homo sapiens. | 4 | EBI-6123119,EBI-366083 |
GO - Molecular functioni
- bHLH transcription factor binding Source: MGI
- identical protein binding Source: MGI
- protein domain specific binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- transcription factor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 204385, 7 interactors |
DIPi | DIP-61633N |
IntActi | P26687, 6 interactors |
STRINGi | 10090.ENSMUSP00000040089 |
Miscellaneous databases
RNActi | P26687, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 112 – 163 | bHLHPROSITE-ProRule annotationAdd BLAST | 52 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 165 – 195 | Sufficient for transactivation activityAdd BLAST | 31 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 80 – 102 | Gly-richAdd BLAST | 23 |
Phylogenomic databases
eggNOGi | KOG4447, Eukaryota |
GeneTreei | ENSGT00940000162831 |
HOGENOMi | CLU_112073_0_0_1 |
InParanoidi | P26687 |
OMAi | MQQDESN |
OrthoDBi | 1595261at2759 |
TreeFami | TF315153 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR015789, Twist-related |
PANTHERi | PTHR23349:SF64, PTHR23349:SF64, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
P26687-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMQDVSSSPV SPADDSLSNS EEEPDRQQPA SGKRGARKRR SSRRSAGGSA
60 70 80 90 100
GPGGATGGGI GGGDEPGSPA QGKRGKKSAG GGGGGGAGGG GGGGGGSSSG
110 120 130 140 150
GGSPQSYEEL QTQRVMANVR ERQRTQSLNE AFAALRKIIP TLPSDKLSKI
160 170 180 190 200
QTLKLAARYI DFLYQVLQSD ELDSKMASCS YVAHERLSYA FSVWRMEGAW
SMSASH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | A → R in AAA40515 (PubMed:1840517).Curated | 1 | |
Sequence conflicti | 91 | G → P in AAA40515 (PubMed:1840517).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63649 Genomic DNA Translation: AAA40514.1 M63650 mRNA Translation: AAA40515.1 BC033434 mRNA Translation: AAH33434.1 BC083139 mRNA Translation: AAH83139.1 |
CCDSi | CCDS25879.1 |
PIRi | I53066 |
RefSeqi | NP_035788.1, NM_011658.2 |
Genome annotation databases
Ensembli | ENSMUST00000049089; ENSMUSP00000040089; ENSMUSG00000035799 |
GeneIDi | 22160 |
KEGGi | mmu:22160 |
UCSCi | uc007niw.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M63649 Genomic DNA Translation: AAA40514.1 M63650 mRNA Translation: AAA40515.1 BC033434 mRNA Translation: AAH33434.1 BC083139 mRNA Translation: AAH83139.1 |
CCDSi | CCDS25879.1 |
PIRi | I53066 |
RefSeqi | NP_035788.1, NM_011658.2 |
3D structure databases
SMRi | P26687 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204385, 7 interactors |
DIPi | DIP-61633N |
IntActi | P26687, 6 interactors |
STRINGi | 10090.ENSMUSP00000040089 |
PTM databases
iPTMneti | P26687 |
PhosphoSitePlusi | P26687 |
Proteomic databases
jPOSTi | P26687 |
PaxDbi | P26687 |
PRIDEi | P26687 |
Protocols and materials databases
Antibodypediai | 11900, 603 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000049089; ENSMUSP00000040089; ENSMUSG00000035799 |
GeneIDi | 22160 |
KEGGi | mmu:22160 |
UCSCi | uc007niw.1, mouse |
Organism-specific databases
CTDi | 7291 |
MGIi | MGI:98872, Twist1 |
Phylogenomic databases
eggNOGi | KOG4447, Eukaryota |
GeneTreei | ENSGT00940000162831 |
HOGENOMi | CLU_112073_0_0_1 |
InParanoidi | P26687 |
OMAi | MQQDESN |
OrthoDBi | 1595261at2759 |
TreeFami | TF315153 |
Miscellaneous databases
BioGRID-ORCSi | 22160, 0 hits in 17 CRISPR screens |
ChiTaRSi | Twist1, mouse |
PROi | PR:P26687 |
RNActi | P26687, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000035799, Expressed in maxillary prominence and 286 other tissues |
Genevisiblei | P26687, MM |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR015789, Twist-related |
PANTHERi | PTHR23349:SF64, PTHR23349:SF64, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TWST1_MOUSE | |
Accessioni | P26687Primary (citable) accession number: P26687 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | August 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot